產(chǎn)品訂購信息：
英文名稱  Anti-TUB 1 

中文名稱   TUB蛋白抗體費用 

別    名  F10B5.4; rd5; TUB 1; TUB; TUB_HUMAN; Tubby homologue; Tubby protein homolog 1; Tubby protein homolog.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Pig, Cow, Horse 

產(chǎn)品類型   一抗  

研究領域    神經(jīng)生物學 轉錄調節(jié)因子 表觀遺傳學

蛋白分子量  predicted molecular weight: 56kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TUB 1 (241-305aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

TUB蛋白抗體費用 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 In contrast to the rapid early-onset weight gain seen in ob/ob mice (1-3), mutations in the tub gene lead to obesity gradually and strongly resemble late-onset obesity as seen in the human population (4). In addition to excessive deposition of adipose tissue, mice with the tub phenotype also suffer retinal degeneration and neurosensory hearing loss (4-6). The tripartite character of tubby phenotype is strikingly similar to human obesity syndromes such as Alstr鰉 (5) and Bardet-Biedl (6). A candidate for the tub gene has been described (4). A G芓 transversion in this candidate gene eliminates a donor splice site in the 3' coding region resulting in a larger transcript containing an unspliced intron (4). A second prematurely truncated mRNA transcript with the unspliced intron was found to be expressed in the brains of tubby mice at a 2-3 fold higher rate as compared to B6 mice (4). It has been postulated that the phenotypic features of tubby mice can be attributed to cellular apoptosis triggered by the expression of a mutated tub gene (4).

Function : Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.

Subunit : Interacts with GNAQ (By similarity). Interacts with TULP1

Subcellular Location : Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.

Similarity : Belongs to the TUB family.

Database links : UniProtKB/Swiss-Prot: P50607.1

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吸引素抗體 Anti-ATRN 0.1ml

Rhesus antibody Rh Phospho-EGFR (Tyr1172) 磷酸化表皮生長因子受體抗體 規(guī)格 0.1ml

M199培養(yǎng)液 250ml 國產(chǎn)

ZNF323 英文名稱： 鋅指蛋白323抗體 0.1ml

DPPA2 英文名稱： 多能發(fā)育相關基因2抗體 0.1ml

吸引素抗體 Anti-ATRN 0.1ml

ERK(Extracellular signal-regulated kinase) 原活化蛋白激酶抗原Multi-class antibodies規(guī)格： 0.5mg

精氨酸加壓素受體2抗體 Anti-AVPR2 0.1ml

Rhesus antibody Rh Phospho-EGFR (Tyr1101) 磷酸化表皮生長因子受體抗體 規(guī)格 0.1ml

1640培養(yǎng)液 250ml 國產(chǎn)

ZNT8 英文名稱： 鋅轉運體8蛋白抗體 0.2ml

DTNB 英文名稱： 肌Dystrobrevin β蛋白抗體 0.2ml

精氨酸加壓素受體2抗體 Anti-AVPR2 0.1ml

Human Immunoglobulin M,IgM ELISA Kit 人免疫球蛋白MMulti-class antibodies規(guī)格： 48T

Anti-phospho-RUNX1/AML1(Ser249) 磷酸化急性髓細胞1蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GRM2/GLUR2 促代謝型谷氨酸受體2抗體 規(guī)格 0.1ml

CEA/CD66(Human carcinoembryonic antign) ELISA Kit 人癌胚抗原 96T

1-Oct 英文名稱： 陽離子轉運器1抗體 0.1ml

phospho-Band3 (Tyr359) 英文名稱： 磷酸化紅細胞陰離子交換蛋白1抗體 0.1ml

Anti-phospho-RUNX1/AML1(Ser249) 磷酸化急性髓細胞1蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

人層連蛋白/板層素(LN)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

豚鼠基質金屬蛋白酶抑制因子1(TIMP-1)免疫試劑盒 guinea pig tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

胎球蛋白A(Fetuin A)ELISA試劑盒 ，英文名： Fetuin A ELISA Kit

Mousetoxicshocksyndrometoxin,TSST-1ELISA試劑盒小鼠毒性休克綜合征毒素1(TSST-1)ELISA試劑盒規(guī)格：96T/48T

DuckImmunoglobulinE,IgEELISA試劑盒鴨免疫球蛋白E(IgE)ELISA試劑盒規(guī)格：96T/48T

HumanAmebiasisELISAKit人阿米巴(Amebiasis)ELISA試劑盒規(guī)格：96T/48T

大鼠脫氧酚/脫氧啉(DPD)ELISA試劑盒 ，英文名： DPD ELISA Kit

人游離特異性抗原(fPSA)ELISA檢測試劑盒HumanFreeProstateSpecificAigen,fPSAELISAKit 96T/48T

倉鼠基質金屬蛋白酶9/明膠酶B(MMP-9/Gelatinase B) 免疫試劑盒 Hamster maix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

CLIAKitforPY(Humanpyridiniumcrosslink/PyriLinks)ELISAKit人交聯(lián)物規(guī)格：48T/96T

石蠟切片脂質過氧化物反式十八碳四烯酸(cis-parinaricacid)熒光染色試劑盒10次

ELISAKitBMP-4人骨成型蛋白4規(guī)格：48T/96T

TUB蛋白抗體費用 大鼠腺苷酸環(huán)化酶1(AC-1)ELISA試劑盒 ，英文名： AC-1 ELISA Kit

人腺相關病毒(AAV)ELISA檢測試劑盒Humanadeno-associatedvirus,AAVELISAKit 96T/48T

大鼠ADM2(ADM2)免疫試劑盒 Rat ADM2,ADM2 ELISA kit

CLIAKitforsCD38(HumanSolubleClusterofdiffereiation38)ELISAKit人可溶性CD38規(guī)格：48T/96T

石蠟切片馮庫薩(VONKOSSA)鈣染色試劑盒50次

ELISAKitMMP-10人基質金屬蛋白酶10規(guī)格：48T/96T

抗體的生物素化標記實驗要點：

1. TUB蛋白抗體費用 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）TUB蛋白抗體費用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。