抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 20號(hào)染色體開放閱讀框11抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-TWA1/C20orf11 

中文名稱   20號(hào)染色體開放閱讀框11抗體品牌 

別    名  Chromosome 20 open reading frame 11; CT011_HUMAN; Protein C20orf11; Twa1; Two hybrid associated protein 1; Two hybrid-associated protein 1 with Ran BP-M; Two hybrid associated protein 1 with RanBPM; Two hybrid-associated protein 1 with RanBPM.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Horse 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 細(xì)胞分化

蛋白分子量  predicted molecular weight: 27kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TWA1/C20orf11 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

20號(hào)染色體開放閱讀框11抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 TWA1 is a 228 amino acid nuclear protein that is highly conserved throughout evolution. TWA1 interacts with Ran BP-M, a protein that is implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis, and the transport of RNA and proteins through the nuclear pore complex. Together, Ran BP-M and TWA1 form a complex with cytoplasmic Muskelin, a mediator of cell spreading. It is thought that this complex plays a role in the Ran GTPase cycle and therefore a potential role in the cell cycle. TWA1 contains a LisH-CTLH motif which is usually found in proteins that are involved in nucleokinesis, chromosome segregation, cell migration and microtubule dynamics. The gene encoding TWA1 maps to human chromosome 20, which comprises approximately 2% of the human genome and contains nearly 63 million bases that encode over 600 genes.

Subunit : Interacts with RANBP9. Part of a complex consisting of RANBP9, MKLN1 and GID8.

Subcellular Location : Nucleus.

Similarity : Belongs to the GID8 family.

Contains 1 CTLH domain.

Contains 1 LisH domain.

Database links : UniProtKB/Swiss-Prot: Q9NWU2.1

抗體的鑒定：

1）20號(hào)染色體開放閱讀框11抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-TRPM2 /FITC 熒光素標(biāo)記瞬時(shí)受體電位離子通道蛋白抗體(M亞家族)IgGMulti-class antibodies規(guī)格： 0.2ml

CaMK2a(calcium/calmodulin-dependent protein kinase II alpha) 鈣/鈣調(diào)素依賴蛋白激酶2α抗原Multi-class antibodies規(guī)格： 0.5mg

尿酸鹽轉(zhuǎn)運(yùn)子抗體 Anti-UAT 0.1ml

SNAI2 英文名稱： 鋅指轉(zhuǎn)錄因子Slug抗體 0.1ml

EPHX2 英文名稱： 胞漿環(huán)氧化物水解酶抗體 0.1ml

Rhesus antibody Rh phospho-ILK-1(Thr173) 磷酸化整合素連接激酶1抗體 規(guī)格 0.1ml

CaMK2a(calcium/calmodulin-dependent protein kinase II alpha) 鈣/鈣調(diào)素依賴蛋白激酶2α抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-PH-4/FITC 熒光素標(biāo)記脯氨酸水解酶4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Mose Anti-rat IgG/Gold 金標(biāo)記小鼠抗大鼠IgG (10nm/15nm)Multi-class antibodies規(guī)格： 2ml

*衍生因子抗體 Anti-FAM3B/PANDER 0.1ml

Goat Anti-rabbit IgG/RBITC 羅丹明標(biāo)記的羊抗兔IgG 0.3ml

Factor I heavy chain 英文名稱： 補(bǔ)體因子I重鏈抗體 0.2ml

Rhesus antibody Rh phospho-SYT1(Ser309) 磷酸化突觸結(jié)合蛋白1抗體 規(guī)格 0.1ml

Mose Anti-rat IgG/Gold 金標(biāo)記小鼠抗大鼠IgG (10nm/15nm)Multi-class antibodies規(guī)格： 2ml

NRP-2(neuropilin-2) 神經(jīng)纖毛蛋白-2(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CD44v5 CD44V5抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh p53R2/RRM2B 核苷酸還原酶M2B抗體 規(guī)格 0.1ml

IFNAR1 Kit Mouse 小鼠 IFNAR1 ELISA配對(duì)抗體 ELISA

TSPAN9 英文名稱： 四分子交聯(lián)體9/四旋蛋白抗體 0.2ml

C5ORF4 英文名稱： 5號(hào)染色體開放閱讀框4抗體 0.2ml

Anti-CD44v5 CD44V5抗體Multi-class antibodies規(guī)格： 0.1ml

TNFSF15 Protein Rat 重組大鼠 TNFSF15 / TL1A 蛋白 (Fc 標(biāo)簽)

FaDu 人咽鱗癌細(xì)胞

Spike Others Human coronavirus 人冠狀病毒 spike glycoprotein (aa 1-760) 人細(xì)胞裂解液 (陽性對(duì)照)

Caco-2人結(jié)直腸腺癌細(xì)胞 Caco-2 human colorectal adenocarcinoma cells MEM+20% FBS

人惡性多發(fā)性;ERA-2

人卵巢微內(nèi)皮細(xì)胞(HOMEC) ( 5×105 ) Saos-2，人成細(xì)胞 Human

CL-0422RBL-1(大鼠嗜堿性粒細(xì)胞性細(xì)胞)5×106cells/瓶×2

CD40LG Others Canine 狗 CD40L / CD154 / TNFSF5 人細(xì)胞裂解液 (陽性對(duì)照)

人基質(zhì)細(xì)胞RNAHHGMC miRNA5 μg

兔腎細(xì)胞;RK-13 細(xì)胞，KG-1細(xì)胞 MFC-GFP細(xì)胞，綠色熒光蛋白標(biāo)記小鼠前細(xì)胞

人類成纖維細(xì)胞系;CNLMG-B5537SKIN

HA Others H3N2 甲型 H3N2 (A/Aichi/2/1968) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽性對(duì)照)

20號(hào)染色體開放閱讀框11抗體品牌 TNFSF15 Protein Rat 重組大鼠 TNFSF15 / TL1A 蛋白 (Fc 標(biāo)簽)

FaDu 人咽鱗癌細(xì)胞

Spike Others Human coronavirus 人冠狀病毒 spike glycoprotein (aa 1-760) 人細(xì)胞裂解液 (陽性對(duì)照)

Caco-2人結(jié)直腸腺癌細(xì)胞 Caco-2 human colorectal adenocarcinoma cells MEM+20% FBS

人惡性多發(fā)性;ERA-2

人卵巢微內(nèi)皮細(xì)胞(HOMEC) ( 5×105 ) Saos-2，人成細(xì)胞 Human