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磷酸化端粒酶逆轉(zhuǎn)錄酶抗體品牌 英文名稱(chēng)  Anti-phospho-TERT(Ser1125)

中文名稱(chēng)  磷酸化端粒酶逆轉(zhuǎn)錄酶抗體品牌 

別    名 p-TERT(Ser1125); TERT(phospho S1125); EST2; hEST2; TCS1; Telomerase associated protein 2; Telomerase Catalytic Subunit; Telomere Reverse Transcriptase; TERT; TP2; TRT.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類(lèi)型   一抗 磷酸化抗體  

研究領(lǐng)域     免疫學(xué) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 124kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human TERT around the phosphorylation site of Ser1125

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化端粒酶逆轉(zhuǎn)錄酶抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Telomerase are large DNA-protein complexes with telomerase expression being the subject of recent research due to its link to cell immortalization. Recent evidence has shown that MYC upregulates the catalytic subunit of telomerase, TERT, and that TERT cooperates with HPV E7 in cell immortalization. Ever since the discovery that telomeres are short in cancer cells and telomerase is activated in immortal cells, telomerase has been associated with oncogenes. During the past year, major advances have been made in understanding the link between telomerase expression and cell immortality. Studies of yeast telomeres have revealed an unexpected role for the non-homologous end-joining machinery in telomere maintenance and have provided the first definitive evidence that telomeres play a critical role in meiosis. Identification of new telomere proteins has led to a better understanding of vertebrate telomere structure and function.

端粒酶逆轉(zhuǎn)錄酶hTERT是構(gòu)成端粒酶的組分之一,是端粒酶活性的必需和限速成分,其水平?jīng)Q定細(xì)胞端粒酶的活性.抑制hTERT可降低端粒酶的活性,從而抑制瘤細(xì)胞生長(zhǎng).目前對(duì)hTERT的研究已成為端粒酶研究的熱點(diǎn)問(wèn)題,已發(fā)現(xiàn)TERT蛋白表達(dá)在診斷中有重要意義,并制備了hTERT抗體及應(yīng)用核酶技術(shù)等來(lái)抑制hTERT蛋白的表達(dá),抑制端粒酶活性,從而的生長(zhǎng).

端粒反轉(zhuǎn)錄酶又稱(chēng)端粒酶催化亞單位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associated protein 2；TP2；Telomerase reverse transcriptase；telomerase catalytic subunit）是細(xì)胞永生化及發(fā)生過(guò)程中的端粒酶活化的主要限速步驟。hTRT基因表達(dá)可以反映端粒酶活性，與端粒酶活性具有平行關(guān)系.

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