產品訂購信息：
英文名稱  Anti-TRPM7 

中文名稱   瞬時受體電位離子通道蛋白7抗體（M亞家族）規(guī)格 

別    名  CHAK 1; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; FLJ20117; FLJ25718; homolog of mouse transient receptor potential-phospholipase C-interacting kinase; Long transient receptor potential channel 7; LTRPC 7; LTRPC ion channel family member 7; LTrpC-7; LTRPC7; transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRP-PLIK; TRPM 7; TRPM7; TRPM7_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

產品類型   一抗  

研究領域    細胞生物 免疫學 神經生物學 信號轉導 通道蛋白

蛋白分子量  predicted molecular weight: 180, 205kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TRPM7 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

瞬時受體電位離子通道蛋白7抗體（M亞家族）規(guī)格 產品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam.[provided by RefSeq, May 2010].

Function : Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).

Subunit : Homodimer. Interacts with PLCB1. Forms heterodimers with TRPM6.

Subcellular Location : Membrane; Multi-pass membrane protein

Post-translational modifications : Autophosphorylated (By similarity).

DISEASE : Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.

Similarity : In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.

In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.

Contains 1 alpha-type protein kinase domain.

Database links :

Entrez Gene: 54822 Human

Entrez Gene: 58800 Mouse

Entrez Gene: 679906 Rat

Omim: 605692 Human

SwissProt: Q96QT4 Human

SwissProt: Q923J1 Mouse

SwissProt: Q925B3 Rat

Unigene: 512894 Human

Unigene: 244705 Mouse

Unigene: 86991 Rat

Anti-TSH/FITC 熒光素標記促甲狀腺素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Tranthyretin 甲狀腺素運載蛋白(抗原)Multi-class antibodies規(guī)格： 0.5mg

包含氧化還原酶的WW域抗體 Anti-WWOX 0.1ml

STARD5 英文名稱： 促黃體激素誘導蛋白5抗體 0.2ml

EphB6 英文名稱： 酪酸蛋白激酶受體B6抗體 0.1ml

Rhesus antibody Rh phospho-IKK beta(Ser476) 磷酸化KB抑制蛋白激酶β抗體 規(guī)格 0.1ml

Tranthyretin 甲狀腺素運載蛋白(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-Os05g/0477200 protein /FITC 熒光素標記抗水稻白葉枯病菌Os05g0477200蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Mouse Anti-goat IgG/HRP 辣根過氧化物酶標小鼠抗羊IgGMulti-class antibodies規(guī)格： 0.1ml

毒蕈堿型乙酰膽堿受體M3抗體 Anti-ChRM3 0.1ml

Mouse Anti-Rabbit IgM/PE-Cy5.5 PE-Cy5.5標記的小鼠抗兔IgM 0.1ml

F1 operon positive regulatory protein(NT) 英文名稱： 肺耶爾森氏菌F1莢膜蛋白抗體(N端) 0.2ml

Rhesus antibody Rh PKA R2/PKA IIα 蛋白激酶A受體2α亞基抗體 規(guī)格 0.2ml

Mouse Anti-goat IgG/HRP 辣根過氧化物酶標小鼠抗羊IgGMulti-class antibodies規(guī)格： 0.1ml

LDL-R(Low-density lipoprotein receptor precursor) 受體(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-CD25/IL-2RA 白介素2受體a鏈抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh PAK6 P21蛋白激活的蛋白激酶6抗體 規(guī)格 0.1ml

EPOR Kit Human 人 EPOR ELISA配對抗體 ELISA

TTC21B 英文名稱： 四聚體多肽蛋白21B抗體 0.2ml

CD24 英文名稱： CD24抗體 0.1ml

Anti-CD25/IL-2RA 白介素2受體a鏈抗體Multi-class antibodies規(guī)格： 0.1ml

TNFSF12 Protein Cynomolgus 重組食蟹猴 / 人 TNFSF12 蛋白 (Fc 標簽)

CW-2 人細胞

BCAM Others Mouse 小鼠 BCAM 人細胞裂解液 (陽性對照)

HT-29人細胞 HT-29 human colon cancer cells McCoy's 5a+10%FBS

人T轉基因細胞;Jurkat D,E

人虹狀體上皮細胞 (HIPEpiC)( 5×105 ) HUM, 正常人主動脈平滑肌細胞 Human

CL-0440SK-MEL-1(人皮膚細胞)5×106cells/瓶×2

TNFSF11 Others Human 人 RANKL / OPGL / TNFSF11 / CD254 人細胞裂解液 (陽性對照)

人上皮細胞RNAHBEpiC miRNA5 μg

BIU-87細胞，人細胞 穩(wěn)定轉染了neor和LIF基因的STO細胞株,SNL細胞 CSC, 小鼠心肌細胞

M1(小鼠細胞) 5×106cells/瓶×2

NHEK adult single donor Pellet 正常人類表皮角質細胞團塊(捐獻者) > 1 mio.cells 小鼠腎小管上皮細胞MREpiC

瞬時受體電位離子通道蛋白7抗體（M亞家族）規(guī)格 TNFSF12 Protein Cynomolgus 重組食蟹猴 / 人 TNFSF12 蛋白 (Fc 標簽)

CW-2 人細胞

BCAM Others Mouse 小鼠 BCAM 人細胞裂解液 (陽性對照)

HT-29人細胞 HT-29 human colon cancer cells McCoy's 5a+10%FBS

人T轉基因細胞;Jurkat D,E

人虹狀體上皮細胞 (HIPEpiC)( 5×105 ) HUM, 正常人主動脈平滑肌細胞 Human

抗體的生物素化標記實驗要點：

1. 瞬時受體電位離子通道蛋白7抗體（M亞家族）規(guī)格 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）瞬時受體電位離子通道蛋白7抗體（M亞家族）規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。