產(chǎn)品訂購信息：
英文名稱  Anti-TGF beta 1 

中文名稱   轉(zhuǎn)化生長因子β1/TGF β1/TGF-β1抗體品牌 

別    名  CED; DPD1; LAP; Latency-associated peptide; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig 

產(chǎn)品類型   一抗  

研究領(lǐng)域    心 信號轉(zhuǎn)導(dǎo) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 12.8kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TGF-Beta 1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

轉(zhuǎn)化生長因子β1/TGF β1/TGF-β1抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Function : Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Subunit : Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN.

Subcellular Location : Secreted, extracellular space, extracellular matrix.

Tissue Specificity : Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

Post-translational modifications : Glycosylated.

The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

DISEASE : Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

Similarity : Belongs to the TGF-beta family.

Database links :

UniProtKB/Swiss-Prot: P01137.2

Entrez Gene: 7040 Human

Entrez Gene: 21803 Mouse

Entrez Gene: 59086 Rat

Omim: 190180 Human

SwissProt: P01137 Human

SwissProt: P04202 Mouse

SwissProt: P17246 Rat

Unigene: 645227 Human

生長因子和激素（ Growth Factor and Hormones） TGF是一種多效生長因子，，可抑制用于許多如:、、、、、等多種的研究。 TGFβ超級家族由為數(shù)眾多的生長的分化因子組成，包括轉(zhuǎn)移生長因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盤生長因子(PTGF-β)；生長/分化因子(GDFs)；繆氏抑制物(MIS)；骨形態(tài)形成蛋白(BMPs)；交織細(xì)胞元神經(jīng)生長因子(GDNF)；抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超級家族成員參與胚胎發(fā)育和成體組織體內(nèi)平衡。TGF-β1抗體與TGF-β2和TGF-β3沒有交叉反應(yīng)。與豚鼠有大部分交叉。

Anti-TNFSF4/CD252/FITC 熒光素標(biāo)記壞死因子配體超家族成員4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

PI3K 磷脂酰肌醇激酶(抗原)Multi-class antibodies規(guī)格： 0.5mg

Toll樣受體5抗體 Anti-TLR5/CD285 0.1ml

5HT2B Receptor 英文名稱： 5-羥色胺受體2B抗體 0.2ml

EP4 英文名稱： 素E受體蛋白4抗體 0.1ml

Rhesus antibody Rh phospho-JunD(Ser255) 磷酸化活化蛋白激酶D抗體 規(guī)格 0.1ml

PI3K 磷脂酰肌醇激酶(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-JNK3/MAPK10/FITC 熒光素標(biāo)記氨基末端激酶3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Mouse Anti-Rabbit IgM 小鼠抗兔IgM (親和層析純化)Multi-class antibodies規(guī)格： 1mg

粒細(xì)胞-巨噬細(xì)胞集落刺激因子抗體 anti-G-CSF 0.1ml

Donkey Anti-rabbit IgG/Alexa Fluor 488 Alexa Fluor 488標(biāo)記的驢抗兔IgG 0.1ml

FIZ1 英文名稱： 鋅指蛋白798抗體 0.2ml

Rhesus antibody Rh Phospho-Smad3(Ser208) 磷酸化細(xì)胞信號轉(zhuǎn)導(dǎo)分子SMAD3抗體 規(guī)格 0.1ml

Mouse Anti-Rabbit IgM 小鼠抗兔IgM (親和層析純化)Multi-class antibodies規(guī)格： 1mg

SMN1(survival of motor neuron 1) 運動神經(jīng)元生存蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-BLNK B淋巴細(xì)胞連接蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh PGBD1/Cerebral protein 4 腦蛋白4抗體 規(guī)格 0.2ml

Dysferlin 濃縮液 0.1ml 進(jìn)口分裝

TWEAK 英文名稱： 壞死因子配體超家族成員12抗體 0.1ml

CK10 英文名稱： 細(xì)胞角蛋白10抗體 0.1ml

Anti-BLNK B淋巴細(xì)胞連接蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

GDNF Protein Rat 重組大鼠 GDNF 蛋白 (His 標(biāo)簽)

B16瘤

PGC Others Rat 大鼠 Pepsinogen C / PGC 人細(xì)胞裂解液 (陽性對照)

A549-EGFP-puro(慢病毒構(gòu)建穩(wěn)定株)人細(xì)胞 A549-EGFP-puro (leiviral consuct stable sain) in human lung cancer cells F-12K+10%FBS+1%P/S+2ug/ml puromycin

大鼠細(xì)胞;CBRH-7919 [CBRH7919]

人腎系膜細(xì)胞 (HRMC)(1×106 ) 細(xì)胞名稱 種屬

CM-H022人成纖維細(xì)胞完全培養(yǎng)基100mL

BBOX1 Others Human 人 BBOX1 / Gamma-BBH 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

成纖維細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

恒河猴腎細(xì)胞;RM-1 人*癌細(xì)胞，HPAC細(xì)胞 SGC-7901(胃腺癌細(xì)胞)

人Wilms細(xì)胞;G401

IL12A Others Human 人 IL12A / NKSF1 人細(xì)胞裂解液 (陽性對照)

轉(zhuǎn)化生長因子β1/TGF β1/TGF-β1抗體品牌 GDNF Protein Rat 重組大鼠 GDNF 蛋白 (His 標(biāo)簽)

B16瘤

PGC Others Rat 大鼠 Pepsinogen C / PGC 人細(xì)胞裂解液 (陽性對照)

A549-EGFP-puro(慢病毒構(gòu)建穩(wěn)定株)人細(xì)胞 A549-EGFP-puro (leiviral consuct stable sain) in human lung cancer cells F-12K+10%FBS+1%P/S+2ug/ml puromycin

大鼠細(xì)胞;CBRH-7919 [CBRH7919]

人腎系膜細(xì)胞 (HRMC)(1×106 ) 細(xì)胞名稱 種屬

抗體的生物素化標(biāo)記實驗要點：

1. 轉(zhuǎn)化生長因子β1/TGF β1/TGF-β1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）轉(zhuǎn)化生長因子β1/TGF β1/TGF-β1抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。