中文名稱  T淋巴細(xì)胞受體α抗體說明書 

英文名稱  Anti-TCR alpha

別    名 T cell antigen receptor alpha polypeptide; T cell receptor alpha; T cell receptor alpha locus; TCRA; TCRD; TRA; PTCRA_HUMAN.

產(chǎn)品屬性：

T淋巴細(xì)胞受體α抗體說明書 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse

產(chǎn)品類型   一抗  

研究領(lǐng)域    發(fā)育生物學(xué) 干細(xì)胞 淋巴細(xì)胞 t-淋巴細(xì)胞

蛋白分子量  predicted molecular weight: 27kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from Human TCR alpha

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

T淋巴細(xì)胞受體α抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Maturation of cytotoxic T lymphocytes is mediated in part by a structure on the T cell surface known as the T cell antigen receptor (TCR) complex. The pre-TCR complex consists of pre-TCR α, pre-TCR βand CD3, which together help coordinate early thymocyte development. Pre-TCR α chain is a type I transmembrane glycoprotein with an extracellular region similar to the constant domain of the immunoglobulin supergene family. Notch3 transgenic mice lacking pre-TCR α inhibits tumor development. In humans, acute lymphoblastic leukemias (ALL) in remission have lower transcript levels of Notch3, HES-1 and pre-TCR α transcripts a and b relative to proliferating ALLs. The human pre-TCR α gene is expressed in immature T cells and maps to chromosome 6p21.3.

Function : TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma and delta chains. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.

Subcellular Location : Membrane; Single-pass type I membrane protein (Potential).

Database links : UniProtKB/Swiss-Prot: Q6ISU1.1

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★T淋巴細(xì)胞受體α抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

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★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

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