抗體的生物素化標記實驗要點：

1. 結(jié)構(gòu)蛋白家族3抗體品牌 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-TCTN3/TECT3 

中文名稱   結(jié)構(gòu)蛋白家族3抗體品牌 

別    名  C10orf61; Chromosome 10 open reading frame 61; DKFZP564D116; TCTN3; TECT3_HUMAN; Tectonic 3; Tectonic 3 precursor; Tectonic family member 3; Tectonic-3.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領域    細胞生物 細胞凋亡 生長因子和激素

蛋白分子量  predicted molecular weight: 64kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TCTN3/TECT3 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

結(jié)構(gòu)蛋白家族3抗體品牌 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Tect3 is a 607 amino acid single-pass type I membrane protein that belongs to the tectonic family and exists as four alternatively spliced isoforms. Tect3 interacts with MKS1 and may be involved in apoptosis regulation. The gene that encodes Tect3 contains approximately 31,560 bases and maps to human chromosome 10q24.1. Spanning nearly 135 million base pairs and encoding nearly 1,200 genes, chromosome 10 makes up approximately 4.5% of the human genome. Several protein-coding genes, including those that encode chemokines, cadherins, excision repair proteins, early growth response factors (Egrs) and fibroblast growth receptors (FGFRs), are located on chromosome 10. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, Cockayne syndrome, multiple endocrine neoplasia type 2 and porphyria. Tetrahydrobiopterin deficiency and a number of syndromes involving defective skull and facial bone fusion are also linked to chromosome 10.

Function : May be involved in apoptosis regulation.

Subunit : Part of the tectonic-like complex (also named B9 complex) (By similarity).

Subcellular Location : Membrane.

DISEASE : Defects in TCTN3 are the cause of orofaciodigital syndrome 4 (OFD4) [MIM:258860]. A form of orofaciodigital syndrome, a group of heterogeneous disorders characterized by malformations of the oral cavity, face and digits, and associated phenotypic abnormalities that lead to the delineation of various subtypes. OFD4 patients have tongue nodules, multiple frenulae, broad flat nose, hypertelorism, and short rib polydactyly with tibial dysplasia (Majewski syndrome). The presence of severe tibial aplasia differentiates OFD4 from OFD1. Additional features of cystic dysplastic kidneys and brain malformation, including occipital encephalocele, are observed in severely affected patients.

Defects in TCTN3 are the cause of Joubert syndrome 18 (JBTS18) [MIM:614815]. A form of Joubert syndrome, a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. JBTS18 patients have vermis agenesis and the molar tooth sign as well as severe kyphoscoliosis. Other features include intrauterine growth retardation, oral anomalies, micrognathism, polydactyly and camptodactyly, joint laxity, horseshoe kidney, and ventricular septal defect. Note=TCTN3-mutated fibroblasts from JBTS18 patients fail to respond to Shh agonists suggesting that at least some of the defects in affected individuals may be secondary to reduced Shh signaling (PubMed:22883145).

Similarity : Belongs to the tectonic family.

Database links : UniProtKB/Swiss-Prot: Q6NUS6.2

抗體的鑒定：

1）結(jié)構(gòu)蛋白家族3抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

Anti-TP II A/TOP2a/FITC 熒光素標記DNA拓普西異構(gòu)酶ⅡA抗體IgGMulti-class antibodies規(guī)格： 0.2ml

SAP-1 (Synapse associated protein-I) peptide 突觸相關(guān)蛋白-1(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg

酪氨酸激酶A抗體 Anti-TrK A 0.1ml

SLU7 英文名稱： SLU7蛋白抗體 0.2ml

EN2 英文名稱： 同源盒蛋白轉(zhuǎn)錄因子EN2抗體 0.1ml

Rhesus antibody Rh phospho-IRS1(Ser612) 磷酸化受體底物-1抗體 規(guī)格 0.1ml

SAP-1 (Synapse associated protein-I) peptide 突觸相關(guān)蛋白-1(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg

anti-Rarres2/TIG2/FITC 熒光素標記視黃酸受體應答蛋白2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Nociceptin receptor 孤菲肽受體(痛敏肽受體)(抗原)Multi-class antibodies規(guī)格： 0.5mg

信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子2抗體 Anti-STAT2 0.2ml

S100A11 英文名稱： S100鈣結(jié)合蛋白A11抗體 0.1ml

EpCAM 英文名稱： 上皮特異性抗原抗體 0.1ml

Rhesus antibody Rh Phospho-MAP2 (Thr1620/1623) 磷酸化微管相關(guān)蛋白2抗體 規(guī)格 0.1ml

Nociceptin receptor 孤菲肽受體(痛敏肽受體)(抗原)Multi-class antibodies規(guī)格： 0.5mg

PAR-1 (Protease-activated receptors-1) 蛋白酶激活受體-1抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-phospho-ATF2(Ser490/498) 磷酸化活化復制因子2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-APG4B(Ser309) 磷酸化自噬相關(guān)蛋白4B抗體 規(guī)格 0.1ml

S100 A4 濃縮液 0.1ml 進口分裝

UBE2E2 英文名稱： 泛素蛋白連接酶2E2抗體 0.2ml

DHRS4L2 英文名稱： 短鏈脫氫酶/還原酶家族4樣2蛋白抗體 0.2ml

Anti-phospho-ATF2(Ser490/498) 磷酸化活化復制因子2抗體Multi-class antibodies規(guī)格： 0.1ml

IL3 Protein Rat 重組大鼠 IL3 / ierleukin 3 蛋白 (His 標簽)

II型膠原酶(含100mL酶解緩沖液) 10mL

HA Others H3N2 甲型 H3N2 (A/Perth/16/2009) 血凝素 (Hemagglutinin / HA) 人細胞裂解液 (陽性對照)

TE-1人細胞 Human esophageal cancer cell line TE-1. RPMI-1640(GIBCO)+10%FBS

SV40轉(zhuǎn)化的非洲綠猴腎細胞;COS-1

人毛發(fā)干細胞(HHDPC)( 5×105 ) MLF, 小鼠淋巴成纖維細胞 Mouse

CM-H077人心室肌細胞完全培養(yǎng)基100mL

CGB5 Others Human 人 CGB5 人細胞裂解液 (陽性對照)

人外根鞘細胞HHORSC

NCL-H548細胞，人*癌細胞 人癌細胞,MDA-MB-231細胞 巨噬細胞培養(yǎng)基MaM

大額牛腎細胞;BFR-K1

LAYN Others Human 人 Layilin 人細胞裂解液 (陽性對照)

結(jié)構(gòu)蛋白家族3抗體品牌 IL3 Protein Rat 重組大鼠 IL3 / ierleukin 3 蛋白 (His 標簽)

II型膠原酶(含100mL酶解緩沖液) 10mL

HA Others H3N2 甲型 H3N2 (A/Perth/16/2009) 血凝素 (Hemagglutinin / HA) 人細胞裂解液 (陽性對照)

TE-1人細胞 Human esophageal cancer cell line TE-1. RPMI-1640(GIBCO)+10%FBS

SV40轉(zhuǎn)化的非洲綠猴腎細胞;COS-1

人毛發(fā)干細胞(HHDPC)( 5×105 ) MLF, 小鼠淋巴成纖維細胞 Mouse