全、新、優(yōu)、品、好四大特點(diǎn)：

轉(zhuǎn)錄因子SP3抗體費(fèi)用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

轉(zhuǎn)錄因子SP3抗體費(fèi)用 英文名稱  Anti-SP3

中文名稱  轉(zhuǎn)錄因子SP3抗體費(fèi)用 

別    名 D130027J01Rik; DKFZp686O1631; GC binding transcription factor Sp 3; GC binding transcription factor Sp3; MGC105187; OTTMUSP00000014207; SP 3; Sp 3 transcription factor; SP3; Sp3 transcription factor; SP3_HUMAN; Specificity protein 3; SPR 2; SPR-2; SPR2; Transcription factor SP 3; Transcription factor SP3; Transcription factor Sp3.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Cow, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 82kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SP3

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

轉(zhuǎn)錄因子SP3抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share simi-lar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the ∫4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 media

Function : Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.

Subunit : Interacts with HLTF; the interaction may be required for basal transcriptional activity of HLTF. Interacts with HDAC1; the interaction deacetylates SP3 and regulates its transcriptional activity. Interacts with HDAC2 (preferably the CK2-phosphorylated form); the interaction deacetylates SP3 and regulates its transcriptional activity. Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a.

Subcellular Location : Nucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.

Tissue Specificity : Ubiquitously expressed.

Post-translational modifications : Not glycosylated.

Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.

Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.

Similarity : Belongs to the Sp1 C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Database links : UniProtKB/Swiss-Prot: Q02447.3

實(shí)驗(yàn)流程：

正在熱銷的相關(guān)產(chǎn)品：

Human echinococcus antibody IgG ELISA Kit 人包蟲抗體IgGMulti-class antibodies規(guī)格： 48T

Anti-phospho-JAK2(Tyr1007+Tyr1008) 磷酸化蛋白酪氨酸激酶JAK-2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh LCMT1 亮氨酸羧基甲基轉(zhuǎn)移酶1抗體 規(guī)格 0.2ml

PGF(Human Prostaglandin F) ELISA Kit 人素F 96T

EMC1 英文名稱： 腺樣癌特異性相關(guān)抗原EMC1抗體 0.1ml

CD44 英文名稱： CD44抗體 0.1ml

Anti-phospho-JAK2(Tyr1007+Tyr1008) 磷酸化蛋白酪氨酸激酶JAK-2抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-TLR5/CD285 /FITC 熒光素標(biāo)記Toll樣受體5抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Nurr1 核受體相關(guān)因子-1(抗原)Multi-class antibodies規(guī)格： 0.5mg

轉(zhuǎn)移生長因子–β1抗體 Anti-TGF-β1 0.1ml

phospho-SLK (Tyr530) 英文名稱： 磷酸化酪酸蛋白激酶Fyn/同步原癌基因抗體 0.1ml

Phospho-EGFR (Ser991) 英文名稱： 磷酸化表皮生長因子受體抗體 0.1ml

Rhesus antibody Rh phospho-LATS2 (Ser83) 磷酸化抑制基因LATS2抗體 規(guī)格 0.1ml

Nurr1 核受體相關(guān)因子-1(抗原)Multi-class antibodies規(guī)格： 0.5mg

Peptide YY 酪酪肽多肽(1-36)Multi-class antibodies規(guī)格： 0.5mg

Anti-Bassoon 鋅指蛋白231抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-5-Lipoxygenase(Ser271) 磷酸化5-脂氧合酶抗體 規(guī)格 0.1ml

Keratin17 濃縮液 0.1ml 進(jìn)口分裝

UBE2V1 英文名稱： 泛素結(jié)合酶E2變異體1抗體 0.2ml

CHSS2 英文名稱： 軟骨素酶2抗體 0.1ml

Anti-Bassoon 鋅指蛋白231抗體Multi-class antibodies規(guī)格： 0.2ml

IL25 Protein Human 重組人 IL25 蛋白 (Fc 標(biāo)簽)

小鼠角膜內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

OSM Others Mouse 小鼠 Oncostatin M / OSM 人細(xì)胞裂解液 (陽性對照)

NCI-H929-UBC-EGFP(穩(wěn)定株)人細(xì)胞 NCI-H929-UBC-EGFP (stable sain) human myeloma cell 1640+20% FBS(熱滅活)

CM-H076人心房肌細(xì)胞完全培養(yǎng)基100mL

人星形膠質(zhì)細(xì)胞 (HA) (1×106 ) 小鼠皮層神經(jīng)膠質(zhì)細(xì)胞(EGFP標(biāo)記) Mouse

CM-H105人皮下脂肪細(xì)胞完全培養(yǎng)基100mL

IFNA4 Others Cynomolgus 食蟹猴 FGFR4 / FGF Receptor 4 人細(xì)胞裂解液 (陽性對照)

人平滑肌細(xì)胞HBSMC

RWPE1細(xì)胞，人上皮細(xì)胞 鼬肺上皮細(xì)胞,MV-1-Lu細(xì)胞 小鼠海馬趾神經(jīng)元MN-h

LA795(小鼠) 5×106cells/瓶×2

Acc-2(人涎腺腺樣囊性癌細(xì)胞) 5×106cells/瓶×2 CM-R035大鼠肝動脈平滑肌細(xì)胞完全培養(yǎng)基100mL 人胚腎上皮細(xì)胞系;KiMA

轉(zhuǎn)錄因子SP3抗體費(fèi)用 IL25 Protein Human 重組人 IL25 蛋白 (Fc 標(biāo)簽)

小鼠角膜內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

OSM Others Mouse 小鼠 Oncostatin M / OSM 人細(xì)胞裂解液 (陽性對照)

NCI-H929-UBC-EGFP(穩(wěn)定株)人細(xì)胞 NCI-H929-UBC-EGFP (stable sain) human myeloma cell 1640+20% FBS(熱滅活)

CM-H076人心房肌細(xì)胞完全培養(yǎng)基100mL

人星形膠質(zhì)細(xì)胞 (HA) (1×106 ) 小鼠皮層神經(jīng)膠質(zhì)細(xì)胞(EGFP標(biāo)記) Mouse

技術(shù)外包服務(wù):

★轉(zhuǎn)錄因子SP3抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。