產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-SCP2/NLTP 

中文名稱   固醇攜帶蛋白2抗體費(fèi)用 

別    名  DKFZp686C12188; DKFZp686D11188; NLTP; Nonspecific lipid transfer protein; NSL TP; OTTHUMP00000010488; Propanoyl CoA C acyltransferase; SCP 2; SCP chi; SCP X; SCP2; SCPchi; SCPX; Sterol carrier protein 2; Sterol carrier protein X; NLTP_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    心 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào) 細(xì)胞周期蛋白 合成與降解 細(xì)胞分化 細(xì)胞骨架 線粒體

蛋白分子量  predicted molecular weight: 59kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SCP2 C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

固醇攜帶蛋白2抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes two proteins: sterol carrier protein X(SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. [provided by RefSeq, Aug 2010]

Function : Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis.

Subunit : Interacts with PEX5.

Subcellular Location : Cytoplasm. Mitochondrion. Note=Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues.

Isoform SCPx: Peroxisome. Note=Interaction with PEX5 is essential for peroxisomal import.

Isoform SCP2: Mitochondrion (Probable).

Tissue Specificity : Liver, fibroblasts, and placenta.

DISEASE : Leukoencephalopathy, with dystonia and motor neuropathy (LDMN) [MIM:613724]: A syndrome characterized by leukoencephalopathy, dystonic head tremor, spasmodic torticollis and reduced tendon reflexes in lower extremities. Additional features include hyposmia, pathologic saccadic eye movements, a slight hypoacusis, accumulation of branched-chain pristanic acid in plasma, and the presence of abnormal bile alcohol glucuronides in urine. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : In the N-terminal section; belongs to the thiolase family.

Contains 1 SCP2 domain.

Database links : UniProtKB/Swiss-Prot: P22307.2

固醇攜帶蛋白2(SCP2)參與了膽固醇的合成、代謝和轉(zhuǎn)運(yùn)過(guò)程.與膽囊固醇結(jié)石形成有關(guān).

Anti-SIP1 /FITC 熒光素標(biāo)記運(yùn)動(dòng)神經(jīng)元存活蛋白結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1) ABL1抗原Multi-class antibodies規(guī)格： 0.5mg

軸索過(guò)度生長(zhǎng)抑制因子受體/Nogo受體抗體 Anti-Nogo R/NGR 0.1ml

SYT3 英文名稱： 突觸結(jié)合蛋白3抗體 0.1ml

ELYS 英文名稱： 轉(zhuǎn)錄因子ELYS蛋白抗體 0.2ml

Rhesus antibody Rh phospho-P53(Thr81) 磷酸化抑制基因P53抗體 規(guī)格 0.1ml

ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1) ABL1抗原Multi-class antibodies規(guī)格： 0.5mg

GZMB/CCPI(Granzyme B) 顆粒酶B抗原Multi-class antibodies規(guī)格： 0.5mg

OSMR OSMR / IL-31RB 兔單抗 (PE) Human

Rhesus antibody Rh phospho-CSF2RB (Tyr766) 磷酸化粒細(xì)胞-巨噬細(xì)胞集落刺激因子受體β抗體 規(guī)格 0.1ml

麗春紅染色液(PVDF或NC膜染色) 100ml 自產(chǎn)

ZBED5 英文名稱： 鋅指蛋白BED5抗體 0.2ml

DOK3 英文名稱： 對(duì)接蛋白3抗體 0.2ml

OSMR OSMR / IL-31RB 兔單抗 (PE) Human

N-ras 原癌基因抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ARF6 ADP核糖基化因子6抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-ATF2(Ser94) 磷酸化活化復(fù)制因子2抗體 規(guī)格 0.1ml

鼠兔通用SP(HRP)試劑盒 140片 進(jìn)口原料

Ubiquitin D 英文名稱： 泛素D抗體 0.2ml

DUSP13 英文名稱： 雙特異性磷酸酶13抗體 0.2ml

Anti-ARF6 ADP核糖基化因子6抗體Multi-class antibodies規(guī)格： 0.2ml

IL17A Protein Human 重組人 IL17 / IL17A 蛋白

小鼠視網(wǎng)膜muller細(xì)胞完全培養(yǎng)基 100mL

ROBO4 Others Mouse 小鼠 ROBO4 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

SW480-EGFP-puro [SW480-pSB4](慢病毒構(gòu)建穩(wěn)定株)人腺癌細(xì)胞 SW480-EGFP-puro [SW480-pSB4] (leiviral consuct stable sain) of human colon adenocarcinoma cells 1640+10% FBS+2μg/ml puromycin

CM-H108人表皮黑色素細(xì)胞完全培養(yǎng)基100mL

人少突膠質(zhì)前體細(xì)胞(HOPC-os)(懸浮生長(zhǎng)) 人間充質(zhì)干細(xì)胞-臍帶 U251人細(xì)胞

CM-H113人表皮角質(zhì)形成細(xì)胞完全培養(yǎng)基100mL

TNFRSF11B Others Cynomolgus 食蟹猴 Osteoprotegerin / TNFRSF11B 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人腎小管上皮細(xì)胞HRPTEpiC

VLcop細(xì)胞，人細(xì)胞 小鼠系,LA795細(xì)胞 小鼠淋巴成纖維細(xì)胞MLF

L W-3A(小鼠皮下結(jié)締組織細(xì)胞) 5×106cells/瓶×2

AAV-293(人胚腎細(xì)胞) 5×106cells/瓶×2 CM-R027大鼠食管上皮細(xì)胞完全培養(yǎng)基100mL 人羊膜細(xì)胞;WISH

固醇攜帶蛋白2抗體費(fèi)用 IL17A Protein Human 重組人 IL17 / IL17A 蛋白

小鼠視網(wǎng)膜muller細(xì)胞完全培養(yǎng)基 100mL

ROBO4 Others Mouse 小鼠 ROBO4 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

SW480-EGFP-puro [SW480-pSB4](慢病毒構(gòu)建穩(wěn)定株)人腺癌細(xì)胞 SW480-EGFP-puro [SW480-pSB4] (leiviral consuct stable sain) of human colon adenocarcinoma cells 1640+10% FBS+2μg/ml puromycin

CM-H108人表皮黑色素細(xì)胞完全培養(yǎng)基100mL

人少突膠質(zhì)前體細(xì)胞(HOPC-os)(懸浮生長(zhǎng)) 人間充質(zhì)干細(xì)胞-臍帶 U251人細(xì)胞

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 固醇攜帶蛋白2抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）固醇攜帶蛋白2抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。