產(chǎn)品訂購信息：
英文名稱  Anti-Separase 

中文名稱   外紡錘體極樣蛋白1抗體規(guī)格 

別    名  Caspase like protein ESPL1; ESP 1; ESP1; ESP-1; ESPL 1; ESPL1; ESPL-1; Extra spindle poles like 1; Extra spindle poles like 1 protein; Separin; Similar to fission yeast cut1and gene; SSE; Separase; ESPL1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學 發(fā)育生物學 信號轉(zhuǎn)導

蛋白分子量  predicted molecular weight: 233kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human ESPL1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

外紡錘體極樣蛋白1抗體規(guī)格 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers.

Function : Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

Subunit : Interacts with PTTG1. Interacts with RAD21.

Subcellular Location : Cytoplasmic and Nuclear

Post-translational modifications : Autocleaves. This function, which is not essential for its protease activity, is unknown.

Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.

Similarity : Belongs to the peptidase C50 family.

Anti-ULBP2/N2DL2/FITC 熒光素標記兔抗人、大、小鼠UL16結(jié)合蛋白2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CD10/CALLA (Neutral Endopeptidase) CD10抗原Multi-class antibodies規(guī)格： 0.5mg

染色盒同源物3抗體 Anti-CBX3 0.1ml

SV2C 英文名稱： 突觸泡蛋白2C抗體 0.2ml

ELL3 英文名稱： 神經(jīng)生長因子調(diào)控抑制蛋白抗體 0.2ml

Rhesus antibody Rh Phospho-Histone H3 (Ser10) 磷酸化組蛋白H3抗體 規(guī)格 0.1ml

CD10/CALLA (Neutral Endopeptidase) CD10抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Tn-C/FITC 熒光素標記腱糖蛋白-C抗體IgGMulti-class antibodies規(guī)格： 0.2ml

PACAP/VIP receptor 腺苷酸環(huán)化酶激活肽/活性腸肽受體(抗原)Multi-class antibodies規(guī)格： 0.5mg

上皮生長因子樣域酪氨酸激酶2 anti-Tie2 0.2ml

SPAG8 英文名稱： 相關(guān)抗原8抗體 0.2ml

E2F6 英文名稱： 轉(zhuǎn)錄因子E2F6抗體 0.1ml

Rhesus antibody Rh phospho-KLF5(Ser311) 磷酸化腸道內(nèi)富含的Kruppel樣因子5抗體 規(guī)格 0.1ml

PACAP/VIP receptor 腺苷酸環(huán)化酶激活肽/活性腸肽受體(抗原)Multi-class antibodies規(guī)格： 0.5mg

ompF(putative outer membrane porin F protein) 小腸耶爾森氏菌膜通道蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ARIP2a 激活素受體2A抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-ASK1(Ser967) 磷酸化細胞凋亡信號調(diào)節(jié)激酶1抗體 規(guī)格 0.2ml

uPA 濃縮液 0.1ml 進口分裝

UBTD1 英文名稱： 泛素結(jié)構(gòu)域蛋白1抗體 0.2ml

DPH2 英文名稱： 白喉酰胺生物合成樣蛋白2抗體 0.2ml

Anti-ARIP2a 激活素受體2A抗體Multi-class antibodies規(guī)格： 0.2ml

IL17RD Protein Canine 重組狗 IL17RD 蛋白 (His 標簽)

小鼠成年表皮角質(zhì)形成層細胞完全培養(yǎng)基 100mL

FAM3B Others Human 人 FAM3B 人細胞裂解液 (陽性對照)

YAC-1小鼠細胞 YAC-1 mouse lymphoma cells RPMI-1640(GIBCO)+10%FBS

CM-R028大鼠食管平滑肌細胞完全培養(yǎng)基100mL

人脈絡(luò)絲表皮細胞 (HCPEpiC)( 5×105 ) 人臍帶血單個核細胞 Saos-2，人成細胞

CM-H127人晶狀體上皮細胞完全培養(yǎng)基100mL

DPYS Others Human 人 DPYS / Dihydropyrimidinase 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

人軟骨細胞HC-a

羅猴胎腎細胞;MA104 腦細胞，C6細胞 NCI-H446 [H446](小細胞細胞)

人細胞;DU 145 [DU145;DU-145]

TNFRSF14 Others Mouse 小鼠 HVEM / TNFRSF14 人細胞裂解液 (陽性對照)

外紡錘體極樣蛋白1抗體規(guī)格 IL17RD Protein Canine 重組狗 IL17RD 蛋白 (His 標簽)

小鼠成年表皮角質(zhì)形成層細胞完全培養(yǎng)基 100mL

FAM3B Others Human 人 FAM3B 人細胞裂解液 (陽性對照)

YAC-1小鼠細胞 YAC-1 mouse lymphoma cells RPMI-1640(GIBCO)+10%FBS

CM-R028大鼠食管平滑肌細胞完全培養(yǎng)基100mL

人脈絡(luò)絲表皮細胞 (HCPEpiC)( 5×105 ) 人臍帶血單個核細胞 Saos-2，人成細胞

抗體的生物素化標記實驗要點：

1. 外紡錘體極樣蛋白1抗體規(guī)格 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）外紡錘體極樣蛋白1抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。