中文名稱  子宮內(nèi)膜抗體說(shuō)明書(shū) 

英文名稱  Anti-sFRP-4

別    名 sFRP-4; Secreted frizzled-related protein 4; Frizzled protein, human endometrium; FrpHE; SFRP4; SFRP4_MOUSE; FRPHE.

產(chǎn)品屬性：

子宮內(nèi)膜抗體說(shuō)明書(shū) 濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    免疫學(xué) 干細(xì)胞

蛋白分子量  predicted molecular weight: 38kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human sFRP-4 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

子宮內(nèi)膜抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. [SUBCELLULAR LOCATION] Secreted. Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Belongs to the secreted frizzled-related protein (sFRP) family.

Function : Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity).

Subcellular Location : Secreted (By similarity).

Tissue Specificity : Expressed the ovary. Localized to granulosa cells of periovulatory follicles and corpora lutea. Weakly expressed in adult tissues including kidney, brain and lung.

Similarity : Belongs to the secreted frizzled-related protein (sFRP) family.

Contains 1 FZ (frizzled) domain.

Contains 1 NTR domain.

Database links : UniProtKB/Swiss-Prot: Q9Z1N6.1

抗子宮內(nèi)膜抗體屬于自身抗體，在正常育齡婦女中也可以檢測(cè)到，但在人群中，特別是患有的婦女中更多見(jiàn)。

正常機(jī)體具有自身免疫，產(chǎn)生極弱的自身抗體，幫助清除體內(nèi)衰老變性的自身成分，但由于某種原因?qū)е聦?duì)自身組織產(chǎn)生過(guò)度免疫應(yīng)答，則會(huì)發(fā)生過(guò)強(qiáng)的一系列免疫反應(yīng)，致使所侵及的組織免疫活性細(xì)胞增多，免疫復(fù)合物沉積，而導(dǎo)致功能改變。當(dāng)這種抗子宮內(nèi)膜抗體由于反復(fù)刺激而大量產(chǎn)生達(dá)到一定的含量時(shí)，可與自身的子宮內(nèi)膜組織發(fā)生抗原抗體結(jié)合反應(yīng)，并激活引起損傷性效應(yīng)，造成子宮內(nèi)膜組織細(xì)胞生化代謝及生理功能的損害，干擾和妨礙精卵結(jié)合，受精卵的著床和胚囊的發(fā)育而導(dǎo)致或。

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技術(shù)外包服務(wù):

★子宮內(nèi)膜抗體說(shuō)明書(shū) 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

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