全、新、優(yōu)、品、好四大特點(diǎn)：

細(xì)胞凋亡抑制因子抗體規(guī)格 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。

細(xì)胞凋亡抑制因子抗體規(guī)格 英文名稱  Anti-Survivin

中文名稱  細(xì)胞凋亡抑制因子抗體規(guī)格 

別    名 API4; API-4; API 4; Apoptosis Inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; BIRC-5; EPR 1; EPR-1; IAP4; IAP-4; IAP 4; SVV; TIAP; BIRC5_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 細(xì)胞凋亡

蛋白分子量  predicted molecular weight: 18.5kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human BIRC5 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

細(xì)胞凋亡抑制因子抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Shepherdin (Survivin variant; baculoviral IAP repeat-containing protein 5 isoform 3; BIRC5). Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. In yeast, BIR domains are involved in regulating cytokinesis. This novel fold is stabilized by zinc tetrahedrally coordinated by one histidine and three cysteine residues and resembles a classical zinc finger.

Survivin is an anti-apoptotic protein, expressed in the G2/M cell cycle phase. At the onset of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptotic function and increased caspase 3 activity. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein. Survivin might affect regulation of neural cell proliferative responses after brain injury. Survivin is also an inhibitor of apoptosis (IAP), or programmed cell death, that is selectively overexpressed in common human cancers.

Function : Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Subunit : Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with JTB. Interacts with CDCA8 and INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S and M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric and monomeric form can interact with DIABLO/SMAC. Interacts with BIRC6/bruce.

Subcellular Location : Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.

Tissue Specificity : Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Post-translational modifications : Ubiquitination is required for centrosomal targeting.

In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities.

Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

Similarity : Belongs to the IAP family.

Contains 1 BIR repeat.

為Survivin家族成員，又稱：細(xì)胞凋亡抑制因子4，主要用于方面的研究。

實(shí)驗(yàn)流程：

正在熱銷的相關(guān)產(chǎn)品：

Anti-phospho-SHC (Tyr349) /FITC 熒光素標(biāo)記磷酸化SH2結(jié)構(gòu)域轉(zhuǎn)化蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Apaf-1 (Apoptosis protease activating factor-1)(NT) 凋亡蛋白活性因子-1(抗原)Multi-class antibodies規(guī)格： 0.5mg

中分子量神經(jīng)絲蛋白抗體 Anti-NF-M 0.1ml

Stra8 英文名稱： 視黃酸激活基因8抗體 0.1ml

ELK1 英文名稱： 細(xì)胞轉(zhuǎn)錄因子ELK1抗體 0.1ml

Rhesus antibody Rh Phospho-p90RSK (Thr573) 磷酸化絲氨酸/蘇氨酸激酶p90RSK蛋白抗體 規(guī)格 0.1ml

Apaf-1 (Apoptosis protease activating factor-1)(NT) 凋亡蛋白活性因子-1(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-S100-A8/MRP8/CFAG/FITC 熒光素標(biāo)記鈣結(jié)合蛋白S100A8抗體IgGMulti-class antibodies規(guī)格： 0.2ml

PAR-2 AP (Protease-activated receptors-2 Activating polypeptide) 蛋白酶激活受體-2激活肽Multi-class antibodies規(guī)格： 0.5mg

巨噬細(xì)胞移動(dòng)抑制因子抗體 Anti-MIF 0.1ml

SCN5A 英文名稱： 電壓門(mén)控通道5α抗體 0.2ml

FBXO21 英文名稱： F-box蛋白21抗體 0.2ml

Rhesus antibody Rh Phospho-PDPK1(Tyr9) 磷酸化3磷酸肌醇依賴性蛋白激酶1抗體 規(guī)格 0.1ml

PAR-2 AP (Protease-activated receptors-2 Activating polypeptide) 蛋白酶激活受體-2激活肽Multi-class antibodies規(guī)格： 0.5mg

PCNA(phosphos Dyr211)peptide 磷酸化增殖細(xì)胞核多肽抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ATF6 活化轉(zhuǎn)錄因子6抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-Androgen Receptor (Ser212) 磷酸化雄激素受體抗體 規(guī)格 0.1ml

Pancreatic Polypeptide 濃縮液 0.1ml 進(jìn)口分裝

UCHL3 英文名稱： 泛素硫酯酶L3抗體 0.2ml

Dmrta1 英文名稱： 性發(fā)育轉(zhuǎn)錄因子蛋白DMO抗體 0.1ml

Anti-ATF6 活化轉(zhuǎn)錄因子6抗體Multi-class antibodies規(guī)格： 0.1ml

IL12B Protein Human 重組人 IL12B / P40 蛋白 (Fc 標(biāo)簽)

小鼠成骨細(xì)胞完全培養(yǎng)基 100mL

JAM3 Others Human 人 JAM3 / JAM-C 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

P388D1小鼠淋巴樣瘤細(xì)胞 P388D1 mouse lymphoid tumor cells RPMI-1640(GIBCO)+90%馬血清+10%FBS

CM-R060大鼠腎動(dòng)脈內(nèi)皮細(xì)胞完全培養(yǎng)基100mL

人腦微內(nèi)皮細(xì)胞 (HBMEC) ( 5×105 ) 大鼠主動(dòng)脈平滑肌細(xì)胞 細(xì)胞名稱

CM-H135人脈絡(luò)膜微細(xì)胞完全培養(yǎng)基100mL

DPYS Others Human 人 DPYS / Dihydropyrimidinase 桿狀病毒-昆蟲(chóng)細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人*內(nèi)皮細(xì)胞HSEC

非洲綠猴腎細(xì)胞(SV40轉(zhuǎn)化);COS-1 [COS1] 轉(zhuǎn)染腺病毒E1A基因的人腎上皮細(xì)胞，293細(xì)胞 QGY-7703(細(xì)胞)

人細(xì)胞;A549 [A-549]

FAS Others Mouse 小鼠 FAS / TNFRSF6 / CD95 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

細(xì)胞凋亡抑制因子抗體規(guī)格 IL12B Protein Human 重組人 IL12B / P40 蛋白 (Fc 標(biāo)簽)

小鼠成骨細(xì)胞完全培養(yǎng)基 100mL

JAM3 Others Human 人 JAM3 / JAM-C 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

P388D1小鼠淋巴樣瘤細(xì)胞 P388D1 mouse lymphoid tumor cells RPMI-1640(GIBCO)+90%馬血清+10%FBS

CM-R060大鼠腎動(dòng)脈內(nèi)皮細(xì)胞完全培養(yǎng)基100mL

人腦微內(nèi)皮細(xì)胞 (HBMEC) ( 5×105 ) 大鼠主動(dòng)脈平滑肌細(xì)胞 細(xì)胞名稱

技術(shù)外包服務(wù):

★細(xì)胞凋亡抑制因子抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。