抗體的生物素化標記實驗要點：

1. 5-羥色胺轉(zhuǎn)運蛋白抗體說明書 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-SLC6A4/5-HTT 

中文名稱   5-羥色胺轉(zhuǎn)運蛋白抗體說明書 

別    名  5HT transporter; 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1; SC6A4_HUMAN; SERT; SLC6A4; Sodium dependent serotonin transporter; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; sodium-dependent serotonin transporter; 5-hydroxytryptamine transporter; 5HT transporter; Na+/Cl- dependent serotonin transporter.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Guinea Pig 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 通道蛋白 細胞膜受體 藥物及化合物 轉(zhuǎn)運蛋白

蛋白分子量  predicted molecular weight: 70kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human 5-HTT C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

5-羥色胺轉(zhuǎn)運蛋白抗體說明書 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008].

Function : Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

Subunit : Monomer or homooligomer (By similarity). Interacts with TGFB1I1. Interacts (via sialylated form) with MYH9. Interacts with SEC23A, SEC24C and INADL. Interacts with NOS1; the interaction may diminish the cell surface localization of SERT in the brain and, correspondingly, reduce serotonin reuptake. Interacts with filamentous actin and STX1A (By similarity). Interacts (via C-terminus) with VIM. Interacts (via C-terminus) with SCAMP2; the interaction is direct and retains transporter molecules intracellularly. Interacts with RAB4 (GTP-bound form); the interaction retains transporter molecules intracellularly.

Subcellular Location : Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.

Tissue Specificity : Expressed in platelets (at protein level).

Post-translational modifications : Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms (By similarity).

Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation (By similarity). Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity.

Similarity : Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Database links : UniProtKB/Swiss-Prot: P31645.1

SERT是一種鈉依賴單胺轉(zhuǎn)運蛋白,由630個氨基酸組成,相對分子質(zhì)量為:70KDa

抗體的鑒定：

1）5-羥色胺轉(zhuǎn)運蛋白抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

PROG ELISA Kit 魚孕激素/孕酮Multi-class antibodies規(guī)格： 48T

Anti-DAPK2/FITC 熒光素標記凋亡相關(guān)蛋白激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CYP4A22 細胞色素P4504A22抗體(脂肪酸Ω羥化酶) 規(guī)格 0.2ml

Goat IgG/Cy5.5 熒光素Cy5.5標記羊IgG(細胞流式同型對照) 0.1ml

Elastin 英文名稱： 項韌帶彈性蛋白抗體 0.1ml

Rhesus antibody Rh ZAP-70 zeta相關(guān)蛋白70抗體 規(guī)格 0.1ml

Anti-DAPK2/FITC 熒光素標記凋亡相關(guān)蛋白激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-TNFAIP1/FITC 熒光素標記壞死因子α誘導蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

PDGF-R-A 血小板源性生長因子受體-A(抗原)Multi-class antibodies規(guī)格： 0.5mg

金屬蛋白酶組織抑制因子-4抗體 Anti-TIMP-4 0.1ml

5HT3D Receptor 英文名稱： 5-羥色胺受體3D抗體 0.2ml

ENAH 英文名稱： ENAH蛋白抗體 0.2ml

Rhesus antibody Rh phospho-KCND2/Kv4.2(Thr602) 磷酸化電壓門控性通道蛋白Kv4.2抗體 規(guī)格 0.1ml

PDGF-R-A 血小板源性生長因子受體-A(抗原)Multi-class antibodies規(guī)格： 0.5mg

phospho-p38 ERK/MAPK14 (pThr180/pTyr182) peptide 磷酸化原活化蛋白激酶p38抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-MASH1 神經(jīng)母細胞特異性轉(zhuǎn)移因子抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-AQP2(Ser269) 磷酸化水通道蛋白2抗體 規(guī)格 0.1ml

Tgase 濃縮液 0.1ml 進口分裝

UEVLD 英文名稱： 泛素結(jié)合酶E2樣蛋白抗體 0.2ml

DIRC2 英文名稱： 干擾蛋白2抗體 0.2ml

Anti-MASH1 神經(jīng)母細胞特異性轉(zhuǎn)移因子抗體Multi-class antibodies規(guī)格： 0.1ml

IL12A & IL12B Protein Mouse 重組小鼠 IL-12 (IL12A & IL12B Heterodimer) 蛋白

小鼠細胞完全培養(yǎng)基 100mL

HA Others H10N9 甲型 H10N9 (A/duck/HongKong/562/1979) 血凝素HA1 (Hemagglutinin) 人細胞裂解液 (陽性對照)

P3X63Ag8小鼠細胞 Mouse myeloma cell line P3X63Ag8 DMEM培養(yǎng)基+10%FBS

CM-R108大鼠腦微內(nèi)皮細胞完全培養(yǎng)基100mL

UMR-106(大鼠細胞) 5×106cells/瓶×2 HUVEC-12, 人臍內(nèi)皮細胞系

CM-M012小鼠肺動脈成纖維細胞完全培養(yǎng)基100mL

TMEM27 Others Human 人 TMEM27 人細胞裂解液 (陽性對照)

軟骨細胞培養(yǎng)基CM

C-33A細胞，人子細胞 兔主動脈平滑肌細胞,CCC-SMC-1細胞 人表皮角質(zhì)細胞-胎兒HEK-f

HFSF(人胚胎眼鞏膜成纖維細胞) 5×106cells/瓶×2

hMoCD14+-PB single donorpre-screened Pellet 人類外周血CD14+單核細胞團塊單一來源，預選型 > 1 mio.cells 人脊髓星形膠質(zhì)細胞RNAHA-sp miRNA5 μg

5-羥色胺轉(zhuǎn)運蛋白抗體說明書 IL12A & IL12B Protein Mouse 重組小鼠 IL-12 (IL12A & IL12B Heterodimer) 蛋白

小鼠細胞完全培養(yǎng)基 100mL

HA Others H10N9 甲型 H10N9 (A/duck/HongKong/562/1979) 血凝素HA1 (Hemagglutinin) 人細胞裂解液 (陽性對照)

P3X63Ag8小鼠細胞 Mouse myeloma cell line P3X63Ag8 DMEM培養(yǎng)基+10%FBS

CM-R108大鼠腦微內(nèi)皮細胞完全培養(yǎng)基100mL

UMR-106(大鼠細胞) 5×106cells/瓶×2 HUVEC-12, 人臍內(nèi)皮細胞系