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磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體規(guī)格 英文名稱  Anti-Phospho-SATB1 (Ser47)

中文名稱  磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體規(guī)格 

別    名 DNA binding protein SATB1; DNA-binding protein SATB1; SATB homeobox 1; SATB1; SATB1_HUMAN; Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA); Special AT rich sequence binding protein 1; Special AT-rich sequence-binding protein 1.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Chicken, Dog, Pig, Cow, Rabbit

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    免疫學(xué) 染色質(zhì)和核信號(hào) 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 86kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human SATB1 around the phosphorylation site of Ser47

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SATB1 selectively binds double-stranded, special AT-rich RNA sequences in which 1 strand exclusively consists of well-mixed A, T, and C nucleotides (ATC sequences). It complexes mainly with p300 and this complex can bind to the gp91phox promoter and repress transcriptional activity. Its ability to repress transcription is regulated by nuclear matrix binding. It is expressed predominantly in the thymus.

Function : Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.

Subunit : Interacts (via DNA-binding domains) with CUX1; leading to inhibit the attachment to DNA (By similarity). Homodimer. Part of the nuclear protein complex gamma-globin promoter and enhancer binding factor (gamma-PE) composed at least by SATB1 and HOXB2. Interaction with CtBP1 when not acetylated stabalizes attachment to DNA and promotes transcription repression. Interacts with PCAF. Interacts with sumoylated PML, HDAC1 and HIV-1 Tat via the PDZ-like dimerization domain. Interacts also with DYNLT3 and POLR2J2. Binds to EP300.

Subcellular Location : Nucleus matrix. Nucleus, PML body. Note=Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies (PML NBs).

Tissue Specificity : Expressed predominantly in thymus.

Post-translational modifications : Sumoylated. Sumoylation promotes cleavage by caspases.

Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.

Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.

Similarity : Belongs to the CUT homeobox family.

Contains 2 CUT DNA-binding domains.

Contains 1 homeobox DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: Q01826.1

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