中文名稱  神經(jīng)組織特異性F肌動(dòng)蛋白結(jié)合蛋白2抗體品牌 

英文名稱  Anti-Spinophilin/Neurabin 2

別    名 FLJ30345; NEB2_HUMAN; Neurabin II; Neurabin-2; Neurabin-II; Neurabin2; NeurabinII; Neurabin II; Neural tissue specific F actin binding protein II; p130; PP1bp134; PPP1R6; PPP1R9; PPP1R9B; Protein phosphatase 1 regulatory subunit 9B; SPINO; Spinophilin.

產(chǎn)品屬性：

神經(jīng)組織特異性F肌動(dòng)蛋白結(jié)合蛋白2抗體品牌 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞粘附分子 細(xì)胞骨架

蛋白分子量  predicted molecular weight: 89kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Spinophilin/Neurabin 2

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

神經(jīng)組織特異性F肌動(dòng)蛋白結(jié)合蛋白2抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Neurabin-II, also called spinophilin, interacts with actin and PP-1 in dendritic spines of the central nervous system (1,2). The gene encoding human neurabin-II maps to chromosome 17q21-q22 (2). The structural characteristics of neurabin-II include one F-actin binding domain at the N-terminal region, a predicted coiled-coil struture at the C-terminal, one PDZ domain at the middle region, and a domain known to interact with transmembrane proteins (1). Neurabin-II bundles actin fliaments in vitro (1). In vivo, spinophilin localizes to the cortical sites of actin filaments and to the sites of active membrane remodelling (4). Neurabin-II also forms a complex with the catalytic subunit of PP1 and modulates PP1 enzymatic activity in vitro (2). Neurabin-II localizes to the head of dendritic spines (2) and aids in the ability of PP-1 to regulate the activity of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-asparate (NMDA) receptors (3). In this manner, neurabin-II modulates both glutamatergic synaptic transmission and dendritic morphology (3). Synergistic interactions between spinophilin and human tumor supressor ARF suggest a role for neurabin-II in cell growth (5).

Function : Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth

Subunit : Interacts with DCLK2 (By similarity). Possibly exists as a homodimer, homotrimer or a homotetramer. Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D(2) dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1. Interacts (via C-terminus) with SPATA13 (via C-terminal tail).

Subcellular Location : Cytoplasm > cytoskeleton. Nucleus. Cell projection > dendritic spine. Cell junction > synapse. Cell junction > adherens junction. Cytoplasm. Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > ruffle membrane. Enriched at synapse and cadherin-based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.

Post-translational modifications : Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1.

Similarity : Contains 1 PDZ (DHR) domain.

Database links : UniProtKB/Swiss-Prot: Q96SB3.2

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★神經(jīng)組織特異性F肌動(dòng)蛋白結(jié)合蛋白2抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

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