產(chǎn)品訂購信息：
英文名稱  Anti-Smac/DIABLO 

中文名稱   線粒體促凋亡蛋白抗體規(guī)格 

別    名  0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog (Drosophila); Diablo homolog (Drosophila); Diablo homolog; Diablo homolog mitochondrial; DIABLO S; DIABLO S; Direct IAP binding protein with low pI; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac; Smac protein; Smac protein; SMAC3; SMAC3;

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse 

產(chǎn)品類型   一抗  

研究領域     細胞生物 細胞凋亡 線粒體

蛋白分子量  predicted molecular weight: 21kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Smac C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

線粒體促凋亡蛋白抗體規(guī)格 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

Function : Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Subunit : Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin.

Subcellular Location : Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.

Tissue Specificity : Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

Post-translational modifications : Ubiquitinated by BIRC7/livin.

DISEASE : Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry.

Database links :

UniProtKB/Swiss-Prot: Q9NR28.1

Entrez Gene: 56616 Human

Entrez Gene: 66593 Mouse

Entrez Gene: 288753 Rat

Omim: 605219 Human

SwissProt: Q9NR28 Human

SwissProt: Q9JIQ3 Mouse

Unigene: 169611 Human

Unigene: 46716 Mouse

Smac（second mitochondria-derived activator of caspase）又稱DIABLO（direct IAP binding protein），是一種線粒體蛋白，也是重要的促細胞凋亡蛋白之一，能夠凋亡，當殺死不正常細胞的機制啟動后，線粒體釋放出Smac蛋白促使受損或不正常細胞凋亡的生化過程就會出現(xiàn)，Smac在誘導細胞凋亡，增加細胞的敏感性及*等方面有一定的意義和應用前景。Smac是第二個線粒體來源的胱氨酸酶激活劑，它通過拮抗或中和一種或幾種凋亡抑制蛋白（IAP）家族成員而發(fā)揮其促凋亡作用。

Anti-Survivin/FITC 綠色熒光素標記Survivin蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

GSK-3 Beta(NT) (Glycogen Synthase Kinase-3 Beta) 糖原合酶激酶-3β(抗原)Multi-class antibodies規(guī)格： 0.5mg

磷酸酯酶-2Ac抗體 Anti-PP2Ac 0.1ml

SPHK1 英文名稱： 鞘醇激酶1抗體 0.2ml

EGFL8 英文名稱： 表皮生長因子樣蛋白8抗體 0.2ml

Rhesus antibody Rh Phospho-MSK1 (Ser376) 磷酸化有絲分裂原和應激活化型蛋白激酶1抗體 規(guī)格 0.1ml

GSK-3 Beta(NT) (Glycogen Synthase Kinase-3 Beta) 糖原合酶激酶-3β(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-PDGF-B/FITC 熒光素標記血小板源性生長因子-B抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti- guinea pig IgG/FITC 熒光素標記兔抗豚鼠IgGMulti-class antibodies規(guī)格： 0.3ml

TNF-α膜受體抗體/DR3 死亡受體3抗體 Anti-DR3/TNF-αR/TRAMP 0.1ml

Rabbit Anti-horse IgG/PE-Cy3 PE-Cy3標記的兔抗馬IgG 0.1ml

FBXO11 英文名稱： F-box蛋白家族FBXO11抗體 0.2ml

Rhesus antibody Rh Phospho-Torc2/Crtc2(Ser171) 磷酸化CREB轉錄共激活因子TORC2抗體 規(guī)格 0.1ml

Rabbit Anti- guinea pig IgG/FITC 熒光素標記兔抗豚鼠IgGMulti-class antibodies規(guī)格： 0.3ml

P19ARF p19ARF基因抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-ARAlpha1/ADRA1B alpha 1能受體抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-arfaptin 2(Ser260) 磷酸化ADP核糖基化因子結合蛋白2抗體 規(guī)格 0.1ml

TIMP-1 濃縮液 0.1ml 進口分裝

Uridine Phosphorylase 1 英文名稱： 尿嘧啶核苷磷酸化酶1抗體 0.2ml

DGCR2 英文名稱： DGCR2蛋白抗體 0.2ml

Anti-ARAlpha1/ADRA1B alpha 1能受體抗體Multi-class antibodies規(guī)格： 0.1ml

IL6 Protein Rat 重組大鼠 IL6 / Ierleukin-6 蛋白

小鼠小腸內(nèi)皮細胞完全培養(yǎng)基 100mL

CD14 Others Human 人 CD14 人細胞裂解液 (陽性對照)

Pig MSC豬骨髓間質(zhì)干細胞 Pig MSC of porcine bone marrow mesenchymal stem cells DMEM低糖+10% FBS+glutamin

CL-0135L1210(小鼠細胞)5×106cells/瓶×2

RM1(大鼠肌肉成纖維樣細胞) 5×106cells/瓶×2 PC-12未分化(大鼠腎上腺嗜鉻細胞瘤)

CM-M095小鼠成年表皮角質(zhì)形成層細胞完全培養(yǎng)基100mL

KLK1 Others Mouse 小鼠 KLK1 / Kallikrein 1 人細胞裂解液 (陽性對照)

星形細胞培養(yǎng)基SteCM-prf

SW620[SW-620]細胞，人腺癌細胞 人腎上腺腺瘤細胞,NCI-H205細胞 原代系膜細胞培養(yǎng)特制添加劑Many types of cells包裝：5/2.5/1ml

豬源細胞

SELPLG Others Human 人 RPSGL-1 人細胞裂解液 (陽性對照)

線粒體促凋亡蛋白抗體規(guī)格 IL6 Protein Rat 重組大鼠 IL6 / Ierleukin-6 蛋白

小鼠小腸內(nèi)皮細胞完全培養(yǎng)基 100mL

CD14 Others Human 人 CD14 人細胞裂解液 (陽性對照)

Pig MSC豬骨髓間質(zhì)干細胞 Pig MSC of porcine bone marrow mesenchymal stem cells DMEM低糖+10% FBS+glutamin

CL-0135L1210(小鼠細胞)5×106cells/瓶×2

RM1(大鼠肌肉成纖維樣細胞) 5×106cells/瓶×2 PC-12未分化(大鼠腎上腺嗜鉻細胞瘤)

抗體的生物素化標記實驗要點：

1. 線粒體促凋亡蛋白抗體規(guī)格 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）線粒體促凋亡蛋白抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。