產(chǎn)品訂購信息：
英文名稱  Anti-SRPX2 

中文名稱   SRPX2蛋白抗體說明書 

別    名  SRPX2 is a 465 amino acid secreted protein expressed in neurons of the brain, including the rolandic area. It has been suggested that SRPX2 enhances cell motility, migration and adhesion through FAK signaling in gastric and other cancer cells. Localized to the cytoplasm, SRPX2 is a ligand for uPAR (urokinase plasminogen activator), a receptor that is a crucial component of the extracellular plasminogen proteolysis system. SRPX2 may be responsible for rolandic seizures (RSs) associated with oral and speech dyspraxia and mental retardation (MR). The involvement of SRPX2 in these disorders suggests an important role for SRPX2 in the perisylvian region critical for language and cognitive development; SRPX2_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat 

產(chǎn)品類型   一抗  

研究領(lǐng)域     神經(jīng)生物學

蛋白分子量  predicted molecular weight: 50kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SRPX2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

SRPX2蛋白抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SRPX2 is a 465 amino acid secreted protein expressed in neurons of the brain, including the rolandic area. It has been suggested that SRPX2 enhances cell motility, migration and adhesion through FAK signaling in gastric and other cancer cells. Localized to the cytoplasm, SRPX2 is a ligand for uPAR (urokinase plasminogen activator), a receptor that is a crucial component of the extracellular plasminogen proteolysis system. SRPX2 may be responsible for rolandic seizures (RSs) associated with oral and speech dyspraxia and mental retardation (MR). The involvement of SRPX2 in these disorders suggests an important role for SRPX2 in the perisylvian region critical for language and cognitive development.

Function : Acts as a ligand for the urokinase plasminogen activator surface receptor. Plays a role in angiogenesis by inducing endothelial cell migration and the formation of vascular network (cords). Involved in cellular migration and adhesion in cancer cells. Increases the phosphorylation levels of FAK. May have a role in the perisylvian region, critical for language and cognitive development.

Subunit : Interacts with ADAMTS4, CTSB and PLAUR. Interacts with PLAUR (via the UPAR/Ly6 domains).

Subcellular Location : Cytoplasm. Secreted.

Tissue Specificity : Expressed in neurons of the rolandic area of the brain (at protein level). Highly expressed in the brain, placenta, lung, trachea, uterus and adrenal gland. Weakly expressed in the peripheral blood, brain and bone marrow. Expressed in numerous cancer cell lines.

DISEASE : Defects in SRPX2 are a cause of bilateral perisylvian polymicrogyria (BPP) [MIM:300388]. BPP is the most common form of polymicrogyria, a malformation of the cortex, in which the brain surface is irregular and the normal gyral pattern replaced by multiple small, partly fused, gyri separated by shallow sulci. BPP results in mild mental retardation, epilepsy and pseudobulbar palsy, causing difficulties with expressive speech and feeding.

Defects in SRPX2 are a cause of rolandic epilepsy with speech dyspraxia and mental retardation X-linked (RESDX) [MIM:300643]. A condition characterized by the association of rolandic seizures with oral and speech dyspraxia, and mental retardation. Rolandic occur during a period of significant brain maturation. During this time, dysfunction of neural network activities such as focal discharges may be associated with specific developmental disabilities resulting in specific cognitive impairments of language, visuo-spatial abilities or attention.

Similarity : Contains 1 HYR domain.

Contains 3 Sushi (CCP/SCR) domains.

Database links : UniProtKB/Swiss-Prot: O60687.1

Anti-Phospho-Tuberin/TSC2 (Ser939) /FITC 熒光素標記磷酸化馬鈴薯球蛋白(性硬化)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

NAIF1 protein( CB12-327) 核凋亡誘導因子蛋白1(多肽)Multi-class antibodies規(guī)格： 0.5mg

抗原抗體 Anti-CA125 0.1ml

SLC*8 英文名稱： 轉(zhuǎn)運體蛋白8抗體 0.1ml

EGF 英文名稱： 表皮生長因子抗體 0.1ml

Rhesus antibody Rh phospho-HSP70(Tyr41) 磷酸化熱休克蛋白70抗體 規(guī)格 0.1ml

NAIF1 protein( CB12-327) 核凋亡誘導因子蛋白1(多肽)Multi-class antibodies規(guī)格： 0.5mg

Anti-phospho-c-Raf (Ser296) /FITC 熒光素標記磷酸化原癌基因c-Raf抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-Biotin 兔抗生物素抗體IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg

GDNF家族受體α-4抗體 Anti-GFRA4 0.2ml

Rabbit Anti-chicken IgG/Alexa Fluor 350 Alexa Fluor 350標記的兔抗雞IgG 0.1ml

FXR2 英文名稱： 脆性X相關(guān)蛋白樣2抗體 0.2ml

Rhesus antibody Rh Phospho-Smad1/5(Ser463/465) 磷酸化細胞信號轉(zhuǎn)導分子Smad-1/5抗體 規(guī)格 0.1ml

Rabbit Anti-Biotin 兔抗生物素抗體IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg

proCNP/NPPC (pro-natriuretic peptide) 促尿排泄肽前體C抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-AQP7 水通道蛋白-7抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-ATG4C(Ser166) 磷酸化自噬相關(guān)蛋白4C抗體 規(guī)格 0.1ml

山羊抗小鼠 IgG(H+L)/HRP 0.1ml 美國Jackson公司分裝

Uromodulin 英文名稱： TH糖蛋白抗體 0.2ml

DNA polymerase alpha 英文名稱： DNA聚合酶α抗體 0.2ml

Anti-AQP7 水通道蛋白-7抗體Multi-class antibodies規(guī)格： 0.2ml

CF Protein Mouse 重組小鼠 CF / Ciliary Neuroophic Factor 蛋白 (His 標簽)

小鼠腸粘膜上皮細胞完全培養(yǎng)基 100mL

SELP Others Human 人 SELP / selectin P / P-selectin 人細胞裂解液 (陽性對照)

3D4/21豬肺泡巨噬細胞 Porcine alveolar macrophage 3D4/21 1640+10% FBS+0.1mM NEAA+1mM 酸

CL-0183PA317(小鼠成纖維細胞)5×106cells/瓶×2

RBL-1(大鼠嗜堿性粒細胞性細胞) 5×106cells/瓶×2 PC-3(人細胞)

CM-M125小鼠牙細胞完全培養(yǎng)基100mL

IGSF8 Others Mouse 小鼠 PGRL / IGSF8 人細胞裂解液 (陽性對照)

小鼠腎實質(zhì)細胞完全培養(yǎng)基 100mL

P19小鼠 P19 mouse teratoma cells a-MEM(肌醇 43.2mg/l,葉酸 8.82mg/l)+7.5%BCS+2.5%FBS

IL12A Protein Cynomolgus 重組食蟹猴 / 恒河猴 IL12A / NKSF1 蛋白 (Fc 標簽)

SW 1990(人*癌細胞) 5×106cells/瓶×2 NCL-H460(非小細胞)

SRPX2蛋白抗體說明書 CF Protein Mouse 重組小鼠 CF / Ciliary Neuroophic Factor 蛋白 (His 標簽)

小鼠腸粘膜上皮細胞完全培養(yǎng)基 100mL

SELP Others Human 人 SELP / selectin P / P-selectin 人細胞裂解液 (陽性對照)

3D4/21豬肺泡巨噬細胞 Porcine alveolar macrophage 3D4/21 1640+10% FBS+0.1mM NEAA+1mM 酸

CL-0183PA317(小鼠成纖維細胞)5×106cells/瓶×2

RBL-1(大鼠嗜堿性粒細胞性細胞) 5×106cells/瓶×2 PC-3(人細胞)

抗體的生物素化標記實驗要點：

1. SRPX2蛋白抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）SRPX2蛋白抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。