技術外包服務:

★DNA結合蛋白2抗體說明書 分子生物學：質粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構建等

★蛋白工程：原核、哺乳動物蛋白表達系統等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構建、細胞RNAi技術等等。

實驗流程：

DNA結合蛋白2抗體說明書 英文名稱  Anti-SATB2

中文名稱  DNA結合蛋白2抗體說明書 

別    名 DNA binding protein SATB2; DNA-binding protein SATB2; FLJ21474; FLJ32076; KIAA1034; MGC119474; MGC119477; SATB family member 2; SATB homeobox 2; SATB2; SATB2_HUMAN; Special AT rich sequence binding protein 2; Special AT-rich sequence-binding protein 2.

產品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Cow, Horse, Sheep

產品類型   一抗  

研究領域    發(fā)育生物學 神經生物學 信號轉導 結合蛋白

蛋白分子量  predicted molecular weight: 83kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SATB2 (451-485aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

DNA結合蛋白2抗體說明書 產品應用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 SATB2 is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.

Function : Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

Subunit : Interacts with ATF4 and RUNX2; resulting in enhanced DNA binding and transactivation by these transcription factors (By similarity). Interacts with PIAS1.

Subcellular Location : Nucleus matrix.

Tissue Specificity : High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

Post-translational modifications : Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.

DISEASE : Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).

Similarity : Belongs to the CUT homeobox family.

Contains 2 CUT DNA-binding domains.

Contains 1 homeobox DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: Q9UPW6.2

全、新、優(yōu)、品、好四大特點：

DNA結合蛋白2抗體說明書 全：公司提供上萬種產品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產品在我司都能找到。

新：產品更新速度較快，基本上每周都有新產品出現。

優(yōu)：產品質量好，投訴比較少。

好：我公司具有優(yōu)質的技術團隊，產品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產品時沒有任何的后顧之憂。

正在熱銷的相關產品：

Anti-HAS/RBITC 紅色羅丹明標記人血清白蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CRMP2/Dpysl2(collapsin response mediator protein-2) 二氫嘧啶酶樣2CRMP2抗原Multi-class antibodies規(guī)格： 0.5mg

α-輔肌動蛋白4抗體 Anti-α-ACTN4 0.1ml

Sin3b 英文名稱： 轉錄抑制蛋白Sin3b抗體 0.2ml

eEF1A2 英文名稱： 翻譯延伸因子EF-1 α2抗體 0.2ml

Rhesus antibody Rh Phospho-FGFR1(Tyr653/654) 磷酸化堿性成纖維細胞生長因子受體1抗體 規(guī)格 0.1ml

CRMP2/Dpysl2(collapsin response mediator protein-2) 二氫嘧啶酶樣2CRMP2抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Phospho-PDGF Receptor alpha(Tyr849)/PDGF Receptor beta (Tyr857)/FITC 熒光素標記磷酸化血小板源性生長因子受體α/β抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-chi IgM/FITC 熒光素標記兔抗雞IgMMulti-class antibodies規(guī)格： 0.3ml

轉錄因子E2F-1抗體 Anti-E2F1 0.1ml

Goat Anti-human IgG/HRP 辣根過氧化物酶標記的羊抗人IgG 0.1ml

FBXL16 英文名稱： FBXL16蛋白抗體 0.2ml

Rhesus antibody Rh phospho-TNIK(Ser764) 磷酸化TRAF2和NCK激酶相互作用蛋白抗體 規(guī)格 0.1ml

Rabbit Anti-chi IgM/FITC 熒光素標記兔抗雞IgMMulti-class antibodies規(guī)格： 0.3ml

PERK(PRKR-like endoplasmic reticulum kinase) 蛋白激酶R樣內質網激酶抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Phospho-Na,K-ATPase alpha-1 (Tyr10) 磷酸化ATP酶蛋白a1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-Alpha synuclein(Ser129) 磷酸化核突觸蛋白α抗體 規(guī)格 0.1ml

NGFR 濃縮液 0.1ml 進口分裝

Uteroglobin 英文名稱： 子宮珠蛋白抗體 0.2ml

DFFB 英文名稱： Caspase 激活的脫氧核糖核酸酶抗體 0.1ml

Anti-Phospho-Na,K-ATPase alpha-1 (Tyr10) 磷酸化ATP酶蛋白a1抗體Multi-class antibodies規(guī)格： 0.1ml

IL36A Protein Mouse 重組小鼠 IL-1F6 / IL-1 epsilon 蛋白

小鼠氣管和上皮細胞完全培養(yǎng)基 100mL

SPINK4 Others Mouse 小鼠 SPINK4 人細胞裂解液 (陽性對照)

C2C12小鼠成肌細胞 C2C12 mouse myoblasts DMEM+10%FBS

CL-0313293FT(人胚腎細胞)5×106cells/瓶×2

NCI-H838(人非小細胞細胞) 5×106cells/瓶×2 A9(皮下結締組織細胞)

CM-R029大鼠腸平滑肌細胞完全培養(yǎng)基100mL

CDH18 Others Cynomolgus 食蟹猴 CDH18 / Cadherin-18 人細胞裂解液 (陽性對照)

人前脂肪細胞-內臟HPA-v

NCI-H1869[H1869]細胞，人細胞 人細胞,BIU-87細胞 人*間充質干細胞HMSC-hp

水貂肺上皮細胞;Mv.1.Lu(NBL-7)

IgG1 Others Mouse 小鼠 IgG1-Fc 人細胞裂解液 (陽性對照)

DNA結合蛋白2抗體說明書 IL36A Protein Mouse 重組小鼠 IL-1F6 / IL-1 epsilon 蛋白

小鼠氣管和上皮細胞完全培養(yǎng)基 100mL

SPINK4 Others Mouse 小鼠 SPINK4 人細胞裂解液 (陽性對照)

C2C12小鼠成肌細胞 C2C12 mouse myoblasts DMEM+10%FBS

CL-0313293FT(人胚腎細胞)5×106cells/瓶×2

NCI-H838(人非小細胞細胞) 5×106cells/瓶×2 A9(皮下結締組織細胞)