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絲氨酸/蘇氨酸蛋白激酶SRPK7抗體費用 英文名稱  Anti-SFRS7/9G8/SRp20

中文名稱  絲氨酸/蘇氨酸蛋白激酶SRPK7抗體費用 

別    名 9G8; AAG3; HSSG1; RBM37; Splicing factor 9G8; Splicing factor, arginine/serine rich 7; ZCCHC20; ZCRB2; SRSF7_HUMAN.

產品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Cow, Zebrafish

產品類型   一抗  

研究領域    細胞生物 神經生物學 信號轉導 表觀遺傳學

蛋白分子量  predicted molecular weight: 27kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SRp20 (2-70aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

絲氨酸/蘇氨酸蛋白激酶SRPK7抗體費用 產品應用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns, and they are required for accurate splice site recognition and the control of alternative splicing (1). Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF (2). The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, and they are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites (3,4). The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport (5). The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites (6).

Function : Splicing factor, arginine/serine rich 7 is required for pre mRNA splicing. Can also modulate alternative splicing in vitro.

Subunit : Found in large molecular weight complexes containingCCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interactswith CCNL2 and CPSF6.

Subcellular Location : Nuclear

Tissue Specificity : Brain, liver, kidney and lung.

Post-translational modifications : Extensively phosphorylated on serine residues in the RSdomain.

Similarity : Belongs to the splicing factor SR family.

Contains 1 CCHC-type zinc finger.

Contains 1 RRM (RNA recognition motif) domain.

Database links : UniProtKB/Swiss-Prot: Q16629.1

實驗流程：

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