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★軸突導向因子SEMA6D抗體說明書 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構(gòu)建等

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軸突導向因子SEMA6D抗體說明書 英文名稱  Anti-SEMA6D

中文名稱  軸突導向因子SEMA6D抗體說明書 

別    名 SEM6D_HUMAN; KIAA1479; Q8NFY4; Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D; Semaphorin 6D.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Pig, Cow, Horse

產(chǎn)品類型   一抗  

研究領(lǐng)域     細胞生物 發(fā)育生物學 神經(jīng)生物學 細胞周期蛋白 細胞粘附分子 細胞分化

蛋白分子量  predicted molecular weight: 118kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SEMA6D

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

軸突導向因子SEMA6D抗體說明書 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Semaphorins are a large family, including both secreted and membrane associated proteins, many of which have been implicated as inhibitors or chemorepellents in axon pathfinding, fasciculation and branching, and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins, like the secreted ones, can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq, Nov 2010]

Function : Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.

Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Cell membrane; Single-pass type I membrane protein.

Isoform 3: Cell membrane; Single-pass type I membrane protein.

Isoform 4: Cell membrane; Single-pass type I membrane protein.

Isoform 5: Cell membrane; Single-pass type I membrane protein.

Isoform 7: Cytoplasm.

Similarity : Belongs to the semaphorin family.

Contains 1 PSI domain.

Contains 1 Sema domain.

Database links : UniProtKB/Swiss-Prot: Q8NFY4.1

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