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實驗流程：

磷酸化鋅指轉(zhuǎn)錄因子Slug抗體規(guī)格 英文名稱  Anti-phospho-SNAI2(Ser155+Ser158+Ser160+Tyr163+Tyr168)

中文名稱  磷酸化鋅指轉(zhuǎn)錄因子Slug抗體規(guī)格 

別    名 SNAI2(phospho S155/S158/S160/Y163/Y168); Snail 2; Snail-2; Neural crest transcription factor Slug; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH 1; SLUGH; SLUGH1; SNAI 2; SNAI2; Snail 2; Snail homolog 2; Snail2; WS 2D; WS2D; SNAI2_HUMAN; Zinc finger protein SNAI2; Protein snail homolog 2; Zinc finger protein SLUG.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白

蛋白分子量  predicted molecular weight: 30kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human SNAI2 around the phosphorylation site of Ser155+Ser158+Ser160+Tyr164+Tyr169

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化鋅指轉(zhuǎn)錄因子Slug抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SNAI2 is a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin. SLUG is involved in epithelial-mesenchymal transitions (EMT) involving E-cadherin repression which is known to play an important role in tumor progression amd neural tube formation. SLUG also has antiapoptotic activity. Mutations in this gene may be associated with sporadic cases of neural tube defects (referenced from Entrez gene).

Function : Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.

Subunit : Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains) (By similarity). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7.

Subcellular Location : Nucleus. Cytoplasm. Note=Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions.

Tissue Specificity : Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level).

Post-translational modifications : GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.

DISEASE : Waardenburg syndrome 2D (WS2D) [MIM:608890]: WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Note=The disease is caused by mutations affecting the gene represented in this entry.

Piebald trait (PBT) [MIM:172800]: Autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the snail C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Database links : UniProtKB/Swiss-Prot: O43623.1

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