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分選連接蛋白6抗體規(guī)格 英文名稱  Anti-SNX6

中文名稱  分選連接蛋白6抗體規(guī)格 

別    名 Sorting Nexin 6; TFAF2; TRAF4 Associated Factor 2; SNX6_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 發(fā)育生物學(xué) 干細(xì)胞

蛋白分子量  predicted molecular weight: 47kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SNX6

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

分選連接蛋白6抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Two related proteins, TRAF1 and TRAF2 (TNF receptor-associated factors 1 and 2, respectively), form a heterodimeric complex that associates with the cytoplasmic domain of the tumor necrosis factor (TNF) receptor type 2 (1). A third member of this family, TRAF3 (also designated CD40bp or CRAF1) associates with the cytoplasmic domain of CD40 (2). Additional membersof the TRAF/CRAF family of signaling intermediates include TRAF4 (also designated CART1), TRAF5 and TRAF6 (3-5). TRAF4 associated factor 2 (TRAF4-AF2), also designated sorting nexin 6 (SNX6), is a member of the sorting nexin family of molecules, which are widely expressed and associate with various receptor (6).

Function : Snx6 is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms.

Subunit : Interacts with CDKN1B (By similarity). Interacts with TGFB receptors. Interacts with dynactin subunit DCTN1. Interacts with SNX1 and SNX2. Interacts with BACE1 and BRMS1.

Subcellular Location : Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Nucleus. Note=Interaction with SNX1 or SNX2 promotes location at endosome membranes. Only a minor proportion is seen in the nucleus.

Similarity : Belongs to the sorting nexin family.

Contains 1 PX (phox homology) domain.

Database links : UniProtKB/Swiss-Prot: Q9UNH7.1

實驗流程：

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技術(shù)外包服務(wù):

★分選連接蛋白6抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。