產(chǎn)品訂購信息：
英文名稱  Anti-SUMF1 

中文名稱   硫酸酯酶修飾因子1抗體規(guī)格 

別    名  MGC150436; AAPA3037; C alpha formylglycine generating enzyme 1; C-alpha-formylglycine-generating enzyme 1; FGE; FGly generating enzyme; MGC131853; Sulfatase modifying factor 1 [Precursor]; Sulfatase-modifying factor 1; SUMF1; SUMF1_HUMAN; UNQ3037.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞

蛋白分子量  predicted molecular weight: 37kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SUMF1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

硫酸酯酶修飾因子1抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SUMF1 is a 374 amino acid alternatively spliced protein that localizes to the lumen of the endoplasmic reticulum and belongs to the sulfatase-modifying factor family. Expressed ubiquitously with highest expression in liver, kidney and pancreas, SUMF1 exists as either a monomer, a homodimer or a heterodimer (with SUMF2) and functions to oxidize sulfatase cysteine residues to an active FGIy residue, thereby playing an important role in sulfatase activity. Defects in the gene encoding SUMF1 are the cause of multiple sulfatase deficiency (MSD), a heterogeneous disorder characterized by metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay.

Function : Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE.

Subunit : Monomer, homodimer and heterodimer with SUMF2.

Subcellular Location : Endoplasmic reticulum lumen.

Tissue Specificity : Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart.

Post-translational modifications : N-glycosylated. Contains high-mannose-type oligosaccharides.

DISEASE : Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive.

Similarity : Belongs to the sulfatase-modifying factor family.

Database links : UniProtKB/Swiss-Prot: Q8NBK3.3

Resistin 抵抗素抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-phospho-Bax(Ser184) 磷酸化Bax抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh PHLP 抑制基因PHLPP抗體 規(guī)格 0.2ml

Keratin10 濃縮液 0.1ml 進(jìn)口分裝

UVRAG 英文名稱： 抑制基因UVRAG抗體 0.2ml

Chorein/VPS13A 英文名稱： 液泡蛋白分選蛋白VPS13A抗體 0.1ml

Anti-phospho-Bax(Ser184) 磷酸化Bax抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-Phospho-FAK (Tyr925) /FITC 熒光素標(biāo)記兔抗人、大、小鼠磷酸化粘著斑激酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-chicken IgG/Gold 金標(biāo)記兔抗雞IgG(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml

角質(zhì)形成細(xì)胞生長(zhǎng)因子受體/纖維母細(xì)胞生長(zhǎng)因子-7抗體 Anti- KGF/FGF-7 0.1ml

Rabbit Anti-Goat IgG/PE PE標(biāo)記的兔抗羊IgG 0.1ml

FOXRED1 英文名稱： 單跨膜蛋白FOXRED1抗體 0.2ml

Rhesus antibody Rh Phospho-Syk (Ty*3) 磷酸化非受體型酪氨酸蛋白激酶抗體 規(guī)格 0.1ml

Rabbit Anti-chicken IgG/Gold 金標(biāo)記兔抗雞IgG(10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml

5HT2B Receptor 英文名稱： 5-羥色胺受體2B抗體 0.2ml

EP4 英文名稱： 素E受體蛋白4抗體 0.1ml

Toll樣受體5抗體 Anti-TLR5/CD285 0.1ml

Anti-TNFSF4/CD252/FITC 熒光素標(biāo)記壞死因子配體超家族成員4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-JunD(Ser255) 磷酸化活化蛋白激酶D抗體 規(guī)格 0.1ml

PI3K 磷脂酰肌醇激酶(抗原)Multi-class antibodies規(guī)格： 0.5mg

Ishikawa, 人細(xì)胞系

NRL1 黑化大家鼠肺成纖維樣細(xì)胞

GHR Others Rat 大鼠 GHR / Growth Hormone Receptor 人細(xì)胞裂解液 (陽性對(duì)照)

大額牛肺細(xì)胞;BFR-L1

腎小管上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

人細(xì)胞;SF767 大鼠腮腺細(xì)胞完全培養(yǎng)基 100mL

615小鼠狀肺腺株;P615

人結(jié)膜纖維原細(xì)胞(HConF)(5×105 )

CM-M027小鼠食管上皮細(xì)胞完全培養(yǎng)基100mL

小鼠樹突狀細(xì)胞細(xì)胞;DCS 小鼠上皮細(xì)胞完全培養(yǎng)基 100mL

小鼠皮層神經(jīng)膠質(zhì)細(xì)胞(EGFP標(biāo)記) Mouse

WFIKKN2 Others Human 人 WFIKKN2 / GASP-1 人細(xì)胞裂解液 (陽性對(duì)照)

硫酸酯酶修飾因子1抗體規(guī)格 Ishikawa, 人細(xì)胞系

NRL1 黑化大家鼠肺成纖維樣細(xì)胞

GHR Others Rat 大鼠 GHR / Growth Hormone Receptor 人細(xì)胞裂解液 (陽性對(duì)照)

大額牛肺細(xì)胞;BFR-L1

腎小管上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

人細(xì)胞;SF767 大鼠腮腺細(xì)胞完全培養(yǎng)基 100mL

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 硫酸酯酶修飾因子1抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）硫酸酯酶修飾因子1抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。