抗體的生物素化標記實驗要點：

1. 分選連接蛋白2抗體費用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-SNX2 

中文名稱   分選連接蛋白2抗體費用 

別    名  MGC5204; SNX 2; SNX2; SNX2_HUMAN; Sorting nexin 2; Sorting nexin-2; Transformation related gene 9 protein; Transformation-related gene 9 protein; TRG 9; TRG-9; TRG9.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 信號轉(zhuǎn)導(dǎo) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 細胞膜受體

蛋白分子量  predicted molecular weight: 58kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from Human SNX2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

分選連接蛋白2抗體費用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Sorting nexin 1 (SNX1) is a member of a large family of hydrophilic proteins that interact with a variety of receptor types and are involved in intracellular trafficking. SNX1 and the related splice variant, SNX1A, bind the epidermal growth factor (EGF) receptor and facilitate its transport to lysosome, thereby contributing to the degradation of the receptor. SNX2 and SNX4 share a high degree of amino acid similarity with SNX1, as they all contain a characteristic phox homology (PX) domain. These proteins are all partially associated with cellular membranes and they likewise associate with EGF, PDGF and insulin receptor tyrosine kinases. These nexins are widely expressed and yet have various tissue distribution patterns. Additionally, the sorting nexins can associate with each other and with a variety of other cellular proteins, suggesting that they exist as part of multi-subunit complexes. The related protein, SNX3, comprises a distinct subgroup of nexins that share less sequence similarity outside of the PX domain and have dramatically different binding affinities for the tyrosine kinase receptors.

Function : May be involved in several stages of intracellular trafficking. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).

Subunit : Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts with FNBP1. Interacts with SNX6.

Subcellular Location : Early endosome membrane; Peripheral membrane protein; Cytoplasmic side.

Similarity : Belongs to the sorting nexin family.

Contains 1 PX (phox homology) domain.

Database links : UniProtKB/Swiss-Prot: O60749.2

抗體的鑒定：

1）分選連接蛋白2抗體費用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

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Anti-Adiponectin Receptor 1 脂聯(lián)素受體-1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-cdc25A (Ser178) 磷酸化細胞分裂周期蛋白25抗體 規(guī)格 0.1ml

甲基綠染色液-綠色 12ml 配合DAB,New Fuchsin，F(xiàn)uchsin使用

VAT 英文名稱： 囊泡乙酰膽堿通道抗體 0.1ml

DUSP5 英文名稱： 雙特異性蛋酸酶5抗體 0.2ml

Anti-Adiponectin Receptor 1 脂聯(lián)素受體-1抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Rb/RB1 protein/FITC 熒光素標記視網(wǎng)膜母細胞瘤相關(guān)蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-Goat IgG Whole serum 兔抗羊IgG抗血清Multi-class antibodies規(guī)格： 1ml

樣生長因子-II抗體 Anti-IGF-II 0.1ml

Mouse Anti-Bov IgG 小鼠抗IgG 1mg

FXYD6 英文名稱： FXYD離子轉(zhuǎn)運調(diào)節(jié)因子6抗體 0.2ml

Rhesus antibody Rh phospho-Pyk2/PTK2B(Tyr402) 磷酸化富含脯氨酸的酪氨酸激酶2抗體 規(guī)格 0.1ml

Rabbit Anti-Goat IgG Whole serum 兔抗羊IgG抗血清Multi-class antibodies規(guī)格： 1ml

SPRR1a 英文名稱： 角質(zhì)蛋白α抗體 0.1ml

EBF2 英文名稱： 早期B淋巴細胞因子2抗體 0.2ml

壞死因子相關(guān)凋亡誘導(dǎo)配體/凋亡素2配體抗體 Anti-TRAIL/Apo2L 0.1ml

Anti-TRAF2/TNF-R2 /FITC 熒光素標記壞死因子受體相關(guān)因子2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-IRS1(Tyr1222) 磷酸化受體底物-1抗體 規(guī)格 0.1ml

BRCA2(Breast cancer susceptbility gene 2) 癌易感基因2抗原Multi-class antibodies規(guī)格： 0.5mg

SKOV3/Taxol-25, 人紫杉醇耐藥細胞株

LLC-MK2 恒河猴腎細胞

CDON Others Human 人 CDON / CDO 人細胞裂解液 (陽性對照)

CM-H027人淋巴成纖維細胞完全培養(yǎng)基100mL

子宮內(nèi)膜上皮細胞Many types of cells包裝：5 × 105次方(1ml)

人細胞;U-2 OS [U2OS;U2-OS;U-2OS] 大鼠肺平滑肌細胞完全培養(yǎng)基 100mL

C57BL/6小鼠T細胞細胞;RMA

NXPH1 Others Mouse 小鼠 Neurexophilin-1 / NXPH1 人細胞裂解液 (陽性對照)

人淋巴內(nèi)皮細胞完全培養(yǎng)基 100mL

CCC-HSF-1細胞，人皮膚成纖維細胞 粘質(zhì)沙雷伯氏菌 中國倉鼠肺細胞;CHL

CXCL11 Protein Human 重組人 I-TAC / CXCL11 蛋白

NCI-N87(人細胞) 5×106cells/瓶×2 人 VSIG8 人細胞裂解液 (陽性對照)

分選連接蛋白2抗體費用 SKOV3/Taxol-25, 人紫杉醇耐藥細胞株

LLC-MK2 恒河猴腎細胞

CDON Others Human 人 CDON / CDO 人細胞裂解液 (陽性對照)

CM-H027人淋巴成纖維細胞完全培養(yǎng)基100mL

子宮內(nèi)膜上皮細胞Many types of cells包裝：5 × 105次方(1ml)

人細胞;U-2 OS [U2OS;U2-OS;U-2OS] 大鼠肺平滑肌細胞完全培養(yǎng)基 100mL