產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-Somatostatin 

中文名稱   生長(zhǎng)抑素抗體費(fèi)用 

別    名  Growth hormone release inhibiting factor; SMS_HUMAN; SMST; SST; Somatostatin-14; Somatostatin 14; Somatostatin14; somatostatin SS14; Somatostatin 28; Somatostatin28; Somatostatin-28; Somatostatin-28; somatostatin SS28.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 生長(zhǎng)因子和激素

蛋白分子量  predicted molecular weight: 3kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Somatostatin 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

生長(zhǎng)抑素抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Somatostatin is a regulatory hormone that is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G protein-coupled somatostatin receptors. This cyclic tetradecapeptide inhibits the secretion of many important hormones, including somatotropin (also designated growth hormone, or GH), insulin and glucagon. Somatostatin is found in both the hypothalamus and pancreas. Somatostatin is thought to be involved in the regulation of insulin synthesis. The hormone somatostatin has active 14 amino acid and 28 amino acid forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. In the cerebellum, Somatostatin-14 and Somatostatin-28 are highly expressed at birth and in the adult stage, respectively. Somatostatin affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. The gene encoding Somatostatin maps to human chromosome 3q28.

Function : The cyclic tetradecapeptide somatostatin is widely distributed throughout the body and is an important regulator of endocrine and nervous system function. It exerts its biologic actions by binding to specific high affinity receptors on the cell surface. Somatostatin inhibits the release of somatotropin. Somatostatin has also been shown in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid. Somatostatin positive cells may also be present in medullary thyroid carcinomas, C cell hyperplasia, thymic and lung tumors and pulmonary small cell carcinomas.

Subcellular Location : Secreted

Similarity : Belongs to the somatostatin family.

Database links : UniProtKB/Swiss-Prot: P61278.1

LFABP/FABP-1(Liver Fatty acid binding protein) *型脂肪酸結(jié)合蛋白抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Adenylate Kinase 1/ AK1 腺苷酸激酶-1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-c-Abl(Tyr276) 磷酸化非受體酪氨酸激酶c-Abl抗體 規(guī)格 0.1ml

AP-Streptavidin專用稀釋液 10ml pH 7.6,高背景或AP存在時(shí)推薦使用

VAX2 英文名稱： 視神經(jīng)視網(wǎng)膜相關(guān)蛋白VAX2抗體 0.2ml

phospho-DAXX (Ser671) 英文名稱： 磷酸化Fas相關(guān)死亡結(jié)構(gòu)域蛋白抗體 0.1ml

Anti-Adenylate Kinase 1/ AK1 腺苷酸激酶-1抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-phospho-P53 (Ser315)/FITC 熒光素標(biāo)記磷酸化抑制基因P53抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-goat IgM/Biotin 生物素化兔抗羊IgMMulti-class antibodies規(guī)格： 0.3ml

兔抗小胸腺DNA 抗體 Anti-CT DNA 0.2ml

Rabbit Anti-mouse IgM/PE-Cy3 PE-Cy3標(biāo)記的兔抗小鼠IgM 0.1ml

FCGBP 英文名稱： Fcγ免疫球蛋白IgG結(jié)合蛋白抗體 0.2ml

Rhesus antibody Rh phospho-YES1(Tyr426) 磷酸化原癌基因酪氨酸蛋白激酶Yes1抗體 規(guī)格 0.1ml

Rabbit Anti-goat IgM/Biotin 生物素化兔抗羊IgMMulti-class antibodies規(guī)格： 0.3ml

SCN4B 英文名稱： 通道亞基β4抗體 0.2ml

Ephrin B3 英文名稱： 酪酸蛋白激酶B3抗體 0.2ml

Trop-2抗體 Anti-Trop-2 0.1ml

Anti-TREM2/FITC 熒光素標(biāo)記兔抗人、大、小鼠髓系細(xì)胞觸發(fā)受體2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-IRS1(Ser302) 磷酸化受體底物-1抗體 規(guī)格 0.1ml

Secretin 分泌素(抗原)Multi-class antibodies規(guī)格： 0.5mg

GH3, 大鼠垂體生長(zhǎng)激素腺瘤

KMB 人胚肺成纖維樣細(xì)胞

HA Others H1N1 甲型 H1N1 (A/England/195/2009) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CM-H091人大隱內(nèi)皮細(xì)胞完全培養(yǎng)基100mL

人微內(nèi)皮細(xì)胞HCoMEC

人癌細(xì)胞;MDA-MB-435S 人視網(wǎng)膜微內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

CL-0010786-O(人腎透明細(xì)胞癌細(xì)胞)5×106cells/瓶×2

IFNG Others Human 人 IFN-gamma / IFNG / γ-IFN CHO細(xì)胞裂解液 (陽(yáng)性對(duì)照)

肝細(xì)胞生長(zhǎng)添加物HGS

牦牛皮膚細(xì)胞;BMU-S1 單核細(xì)胞巨噬細(xì)胞，J774A1細(xì)胞 Raji(Butt's 細(xì)胞)

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KM9803

JAG1 Others Human 人 JAG1 / JAGL1 / CD339 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

生長(zhǎng)抑素抗體費(fèi)用 GH3, 大鼠垂體生長(zhǎng)激素腺瘤

KMB 人胚肺成纖維樣細(xì)胞

HA Others H1N1 甲型 H1N1 (A/England/195/2009) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CM-H091人大隱內(nèi)皮細(xì)胞完全培養(yǎng)基100mL

人微內(nèi)皮細(xì)胞HCoMEC

人癌細(xì)胞;MDA-MB-435S 人視網(wǎng)膜微內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 生長(zhǎng)抑素抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）生長(zhǎng)抑素抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。