產(chǎn)品訂購信息：
英文名稱  Anti-Syntrophin-3 

中文名稱   互養(yǎng)蛋白3抗體品牌 

別    名  SNTB2_HUMAN; Beta-2-syntrophin; 59 kDa dystrophin-associated protein A1 basic component 2; Syntrophin 3; SNT3; Syntrophin-like; SNTL; SNTB2; D16S2531E; SNT2B2; SNTL; EST25263; SNT3.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 結(jié)合蛋白 跨膜蛋白 細(xì)胞膜蛋白

蛋白分子量  predicted molecular weight: 59kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Syntrophin-3 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

互養(yǎng)蛋白3抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq, Jul 2008].

Function : Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May play a role in the regulation of secretory granules via its interaction with PTPRN.

Subunit : Monomer and homodimer (Probable). Interacts with the other members of the syntrophin family: SNTA1 and SNTB1; and with the sodium channel proteins SCN4A and SCN5A. Interacts with SAST, MAST205, microtubules and microtubule-associated proteins. Interacts with the dystrophin protein DMD and related proteins DTNA and UTRN, and with the neuroregulin receptor ERBB4. Interacts with PTPRN when phosphorylated, protecting PTPRN from protein cleavage by CAPN1. Dephosphorylation upon insulin stimulation disrupts the interaction with PTPRN and results in the cleavage of PTPRN.

Subcellular Location : Membrane. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Cell junction. Cytoplasm, cytoskeleton. Note=Membrane-associated. In muscle, it is exclusively localized at the neuromuscular junction. In insulinoma cell line, it is enriched in secretory granules.

Tissue Specificity : Ubiquitous. Isoform 1 is the predominant isoform. Weak level of isoform 2 is present in all tested tissues, except in liver and heart where it is highly expressed.

Post-translational modifications : Phosphorylated. Partially dephosphorylated upon insulin stimulation.

Similarity : Belongs to the syntrophin family.

Contains 1 PDZ (DHR) domain.

Contains 2 PH domains.

Contains 1 SU (syntrophin unique) domain.

Database links : UniProtKB/Swiss-Prot: Q13425.1

NCBI Reference Sequence: NP_006741.1

Integrin AlphaE2/CD103 整合素αE2抗原Multi-class antibodies規(guī)格： 0.2ml

Anti-ADM/AM 腎上腺髓質(zhì)素抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Phospho-cdc2 (Tyr15) 磷酸化周期素依賴激酶2抗體 規(guī)格 0.1ml

X-GAL染色試劑盒 50ml Sigma原料

VDAC3 英文名稱： 線粒體外膜孔蛋白3抗體(電壓依賴陰離子通道蛋白3) 0.2ml

Drosha 英文名稱： 核糖核酸酶3/Drosha抗體 0.2ml

Anti-ADM/AM 腎上腺髓質(zhì)素抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-phospho-CaMK2a (pThr286)/FITC 熒光素標(biāo)記抗磷酸化鈣/鈣調(diào)素依賴蛋白激酶2α抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-Guinea IgG/Gold 金標(biāo)記兔抗豚鼠IgG (10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml

成纖維細(xì)胞生長(zhǎng)因子受體4抗體 Anti-FGFR4 0.1ml

Goat Anti-Mouse IgG/PE-CY5 PE-CY5標(biāo)記的羊抗小鼠IgG 0.1ml

phospho-Fos B (Ser27) 英文名稱： 磷酸化癌基因FOS蛋白B抗體 0.1ml

Rhesus antibody Rh phospho-STAT6(Tyr641) 磷酸化信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子6抗體 規(guī)格 0.1ml

Rabbit Anti-Guinea IgG/Gold 金標(biāo)記兔抗豚鼠IgG (10nm/15nm)Multi-class antibodies規(guī)格： 0.5ml

STIP1 英文名稱： 應(yīng)激誘導(dǎo)磷蛋白1抗體 0.2ml

Endomucin 英文名稱： 內(nèi)皮粘蛋白EMCN抗體 0.2ml

阻抑基因1抗體 Anti-TSLC1 0.2ml

Anti-TRH/FITC 熒光素標(biāo)記促甲狀腺素釋放激素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-IRAK1 (Thr387) 磷酸化白介素-1受體相關(guān)激酶1抗體 規(guī)格 0.1ml

CA15-3 peptide 癌相關(guān)抗原抗原Multi-class antibodies規(guī)格： 0.5mg

KM3, 人細(xì)胞

HSCT6 大鼠肝星狀細(xì)胞

FAM3D Others Human 人 FAM3D 人細(xì)胞裂解液 (陽性對(duì)照)

CM-R003大鼠Ⅱ型肺泡上皮細(xì)胞完全培養(yǎng)基100mL

人主動(dòng)脈內(nèi)皮細(xì)胞HAEC

細(xì)胞 人腦微內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

CL-0023Ana-1(小鼠巨噬細(xì)胞)5×106cells/瓶×2

TDGF1 Others Rat 大鼠 Cripto / TDGF1 人細(xì)胞裂解液 (陽性對(duì)照)

小鼠卵巢顆粒細(xì)胞完全培養(yǎng)基 100mL

MLTC-1小鼠間質(zhì)細(xì)胞瘤細(xì)胞 MLTC-1 mouse Leydig cell tumor RPMI-1640(GIBCO)+10%FBS

IL22 Protein Human 重組人 IL22 / IL-22 / Ierleukin 22 蛋白

SK-NEP-1(人腎母細(xì)胞瘤細(xì)胞) 5×106cells/瓶×2 PT-67(病毒轉(zhuǎn)染小鼠細(xì)胞)

互養(yǎng)蛋白3抗體品牌 KM3, 人細(xì)胞

HSCT6 大鼠肝星狀細(xì)胞

FAM3D Others Human 人 FAM3D 人細(xì)胞裂解液 (陽性對(duì)照)

CM-R003大鼠Ⅱ型肺泡上皮細(xì)胞完全培養(yǎng)基100mL

人主動(dòng)脈內(nèi)皮細(xì)胞HAEC

細(xì)胞 人腦微內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 互養(yǎng)蛋白3抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）互養(yǎng)蛋白3抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。