磷酸化原癌基因c-Met相關(guān)酪氨酸激酶抗體規(guī)格 英文名稱  Anti-phospho-Ron/MST1R(Ser1349)

中文名稱  磷酸化原癌基因c-Met相關(guān)酪氨酸激酶抗體規(guī)格 

別    名 MST1R(phospho S1349); c met related tyrosine kinase; CD136; CD136 antigen; CDw136; Macrophage stimulating 1 receptor (c met related tyrosine kinase); Macrophage stimulating 1 receptor; Macrophage stimulating protein receptor alpha chain; MACROPHAGE STIMULATING PROTEIN RECEPTOR; Macrophage stimulating protein receptor beta chain; Macrophage-Stimulating 1 Receptor (MST1R); Macrophage-stimulating protein receptor beta chain; MSP receptor; Mst1r; MST1R variant RON30; MST1R variant RON62; p185 RON; p185-Ron; Protein-tyrosine kinase 8; PTK 8; ptk8; PTK8 protein tyrosine kinase 8; Recepteur d’origine nantais (RON); RON; RON protein tyrosine kinase; RON variant E2E3; RON_MOUSE; Soluble RON variant 1; Soluble RON variant 2; Soluble RON variant 3; Soluble RON variant 4; Stem cell derived tyrosine kinase.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Mouse

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    細胞生物 信號轉(zhuǎn)導(dǎo) 細胞周期蛋白 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 30/117/148kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from mouse MST1R around the phosphorylation site of Ser1349

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化原癌基因c-Met相關(guān)酪氨酸激酶抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 MST1R/Ron, a HGF Receptor/MET-type protein kinase, mediates the biological activities of macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival. The protein is a membrane-spanning, disulfide-linked heterodimer, which results from cleavage of a glycosylated precursor into 35-kD (alpha) and 150-kD (beta) subunits. Ligand binding results in tyrosine phosphorylation of the beta chain. In knockout studies, MST1R/RON (-/-) mice failed to survive past the periimplantation period. The MST1R/RON gene has been mapped to 3p21, a region of frequent deletion or mutation in small cell lung and renal carcinoma, and has been implicated in the progression of several epithelial cancers. Ron expression has been documented in many normal human tissues. ESTs have been isolated from several tissue libraries, including normal colon, mouth, prostate, and testis and cancerous colon, prostate, stomach, and uterus.

Function : Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

Subunit : Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion. Isoform sf-Stk forms covalent heterodimers with friend spleen focus-forming virus (FSFFV) gp55.

Subcellular Location : Membrane; Single-pass type I membrane protein.

Tissue Specificity : Expressed in colon, skin, lung and bone marrow.

Post-translational modifications : Proteolytic processing yields the two subunits.

Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.

Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.

Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.

Contains 3 IPT/TIG domains.

Contains 1 protein kinase domain.

Contains 1 Sema domain.

Database links : UniProtKB/Swiss-Prot: Q62190.3

實驗流程：

全、新、優(yōu)、品、好四大特點：

磷酸化原癌基因c-Met相關(guān)酪氨酸激酶抗體規(guī)格 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

LH/CG Receptor(Luteinizing Hormone/Choriogonadotropin Receptor) 人促黃體生成素受體抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Agrin 聚集蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-CaMK2 alpha (Tyr231) 磷酸化鈣/鈣調(diào)素依賴蛋白激酶2α抗體 規(guī)格 0.1ml

堿性磷酸酶阻斷劑 10ml Sigma原料

VGLUT1/BNP1 英文名稱： 腦特異性依賴無機磷轉(zhuǎn)運蛋白抗體 0.2ml

DEGS1 英文名稱： 退行性精母細胞同源物1抗體 0.2ml

Anti-Agrin 聚集蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Oct-4 /FITC 熒光素標(biāo)記胚胎干細胞關(guān)鍵蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-human IgG/HRP 辣根過氧化物酶標(biāo)記兔抗人IgGMulti-class antibodies規(guī)格： 0.1ml

周期素依賴性激酶8抗體 Anti-CDK8 0.2ml

Rabbit Anti-horse IgM/FITC FITC標(biāo)記的兔抗馬IgM 0.1ml

Factor VII 英文名稱： 凝血因子7抗體 0.1ml

Rhesus antibody Rh Plakophilin 1 橋粒斑菲素蛋白1抗體 規(guī)格 0.2ml

Rabbit Anti-human IgG/HRP 辣根過氧化物酶標(biāo)記兔抗人IgGMulti-class antibodies規(guī)格： 0.1ml

Stanniocalcin 1 英文名稱： 斯鈣素抗體 0.1ml

Exportin 1/CRM1 英文名稱： 染色體區(qū)域穩(wěn)定蛋白/核輸出蛋白1抗體 0.1ml

ZCWCC1抗體 Anti-ZCWCC1 0.2ml

Anti-TSP-1/THBS1/FITC 熒光素標(biāo)記血小板反應(yīng)蛋白/凝血酶敏感蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-IKK alpha/beta (Ser176/Ser180) 磷酸化KB抑制蛋白激酶α/β抗體 規(guī)格 0.1ml

Trk B 神經(jīng)生長因子受體的一種(抗原)Multi-class antibodies規(guī)格： 0.5mg

RGC-5, 小鼠視網(wǎng)膜神經(jīng)節(jié)細胞

CSSTH1 中華鱉心肌來源細胞

RSPO1 Others Human 人 RSPO1 / R-Spondin-1 (aa 1-146) 人細胞裂解液 (陽性對照)

T-107B中性蛋白酶(含100mL酶解緩沖液)10mL

黑色素細胞生長生長添加物MelGS

EB病毒轉(zhuǎn)化的人B淋巴細胞;Mao 人骨骼肌細胞完全培養(yǎng)基 100mL

CL-0080WB-F344(大鼠肝上皮樣干細胞)5×106cells/瓶×2

AKT3 Others Human 人 AKT3 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

SMCFectagen? 平滑肌細胞轉(zhuǎn)染試劑盒

LoVo細胞，人細胞 HEK293,人胚腎上皮細胞,293A細胞 腎動脈平滑肌細胞Many types of cells包裝：5 × 105次方(1ml)

家兔腎細胞;RABK3

SFRP4 Others Mouse 小鼠 sFRP4 人細胞裂解液 (陽性對照)

磷酸化原癌基因c-Met相關(guān)酪氨酸激酶抗體規(guī)格 RGC-5, 小鼠視網(wǎng)膜神經(jīng)節(jié)細胞

CSSTH1 中華鱉心肌來源細胞

RSPO1 Others Human 人 RSPO1 / R-Spondin-1 (aa 1-146) 人細胞裂解液 (陽性對照)

T-107B中性蛋白酶(含100mL酶解緩沖液)10mL

黑色素細胞生長生長添加物MelGS

EB病毒轉(zhuǎn)化的人B淋巴細胞;Mao 人骨骼肌細胞完全培養(yǎng)基 100mL

技術(shù)外包服務(wù):

★磷酸化原癌基因c-Met相關(guān)酪氨酸激酶抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構(gòu)建、細胞RNAi技術(shù)等等。