產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-RPS6KB1 

中文名稱   核糖體S6蛋白激酶抗體規(guī)格 

別    名  RPS6KB1; PS6K; S6K; S6K1; STK14A; p70(S6K)-alpha; p70-S6K; p70-alpha; ribosomal protein S6 kinase; 70kDa; p70 S6 Kinase; P70 Beta1; KS6B1_HUMAN; Ribosomal protein S6 kinase beta-1; S6K-beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 58kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from the middle of human p70 S6 Kinase 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

核糖體S6蛋白激酶抗體規(guī)格 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].

Function : Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin.

Subunit : Interacts with PPP1R9A/neurabin-1. Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with POLDIP3 and TRAF4.

Subcellular Location : Cell junction, synapse, synaptosome.Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion. Isoform Alpha I: Nucleus. Cytoplasm. Isoform Alpha II: Cytoplasm.

Tissue Specificity : Widely expressed.

Post-translational modifications : Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: P23443.2

N-cadherin N-鈣粘附分子抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-Apelin receptor Apelin receptor抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-Bad(Ser112) 磷酸化相關(guān)死亡促進(jìn)因子抗體 規(guī)格 0.1ml

山羊抗兔 IgG(H+L)/FITC 0.1ml 綠色熒光，Jackson公司分裝

Von Willebrand Factor 英文名稱： 假性因子/性因子抗體 0.1ml

Dppa4 英文名稱： 多能發(fā)育相關(guān)基因4抗體 0.1ml

Anti-Apelin receptor Apelin receptor抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Pin1/FITC 熒光素標(biāo)記肽基脯氨酞順反異構(gòu)酶Pinl抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-MK IgM 兔抗猴IgM (親和層析純化)Multi-class antibodies規(guī)格： 1mg

代謝型谷氨酸受體5 Anti-GRM5 0.2ml

Dog IgM/PE-Cy3 PE-Cy3標(biāo)記的兔抗犬IgM抗體 0.1ml

FAM156A 英文名稱： 跨膜蛋白29抗體 0.2ml

Rhesus antibody Rh phospho-SCNN1B(Ser633) 磷酸化上皮通道β2抗體 規(guī)格 0.1ml

Rabbit Anti-MK IgM 兔抗猴IgM (親和層析純化)Multi-class antibodies規(guī)格： 1mg

STK2 英文名稱： 絲酸/蘇酸蛋白激酶2抗體 0.2ml

EBNA 3A 英文名稱： EB病毒核抗原-3A抗體 0.2ml

半胱胺酸蛋白酶蛋白-6抗體(C端) Anti-Caspase-6 0.1ml

Anti-Phospho-Tyk2 (Tyr1054/1055)/FITC 熒光素標(biāo)記磷酸化非受體酪氨酸蛋白激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-HRH1(Ser398) 磷酸化組胺受體H1抗體 規(guī)格 0.1ml

CCL12/MCP-5(C-C motif chemokine 12) 單核細(xì)胞趨化蛋白5抗原Multi-class antibodies規(guī)格： 0.5mg

小鼠骨髓間充質(zhì)干細(xì)胞(BMSCs)

BABL/3T3 BABL/C小鼠胚胎成纖維細(xì)胞

IL13 Others Cynomolgus 食蟹猴 IL-13 / Ierleukin-13 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0182P815(小鼠肥大細(xì)胞瘤細(xì)胞)5×106cells/瓶×2

人臍帶間充質(zhì)干細(xì)胞裂解物HUMSCL

人整合SV40基因的上皮細(xì)胞;HBL-100 [HBL100] 人腎動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基 100mL

CL-0128JeKo-1(人套細(xì)胞細(xì)胞)5×106cells/瓶×2

RSV-F Others Respiratory syncytial virus/RSV 人類合胞病毒 hRSV (A2) Fusion glycoprotein / RSV-F 桿狀病毒-昆蟲細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人肺動(dòng)脈平滑肌細(xì)胞裂解物HPASMCL

PC-3細(xì)胞，人細(xì)胞 人細(xì)胞,Ke-3細(xì)胞 臍帶單核細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

兔主動(dòng)脈平滑肌細(xì)胞;CCC-SMC-2

TFPI Others Mouse 小鼠 TFPI / LACI / EPI 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

核糖體S6蛋白激酶抗體規(guī)格 小鼠骨髓間充質(zhì)干細(xì)胞(BMSCs)

BABL/3T3 BABL/C小鼠胚胎成纖維細(xì)胞

IL13 Others Cynomolgus 食蟹猴 IL-13 / Ierleukin-13 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0182P815(小鼠肥大細(xì)胞瘤細(xì)胞)5×106cells/瓶×2

人臍帶間充質(zhì)干細(xì)胞裂解物HUMSCL

人整合SV40基因的上皮細(xì)胞;HBL-100 [HBL100] 人腎動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基 100mL

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 核糖體S6蛋白激酶抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）核糖體S6蛋白激酶抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。