抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 環(huán)指蛋白17抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-RNF17 

中文名稱(chēng)   環(huán)指蛋白17抗體品牌 

別    名  Mmip 2; Mmip2; Ring finger protein 17; RNF 17; SPATA 23; SPATA23; Spermatogenesis associated 23; TDRD 4; TDRD4; Tudor domain containing 4.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse, Sheep 

產(chǎn)品類(lèi)型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號(hào)

蛋白分子量  predicted molecular weight: 185kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RNF17 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

環(huán)指蛋白17抗體品牌 產(chǎn)品應(yīng)用    ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF17 (ring finger protein 17) or tudor domain-containing protein 4, TDRD4, SPATA23, Mmip-2 or FLJ11045, is a testis-specific protein and and novel key regulator of spermiogenesis containing 1,623 amino acids. By distributing Mad proteins to the cytoplasm, RNF17 regulates the transcriptional activity of c-Myc. Although showing localization in the nucleus, RNF17 is predominantly observed in cytoplasm and is a component of a novel nuage found in male germ cells. The gene encoding RNF17 maps to human chromosome 13q12.12 and encodes one RING-type zinc finger and four tudor domains. As a result of alternative splice events, five RNF17 isoforms exisit.

Function : Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad-MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis (By similarity).

Subunit : Interacts with MXD1, MXD3, MXD4, MXI1 and PIWIL1. Self-associates (By similarity).

Subcellular Location : Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly found in the cytoplasm. Component of a nuage in male germ cells (an electron-dense spherical cytoplasmic body present in late pachytene and diplotene spermatocytes and in elonging spermatids) (By similarity).

Tissue Specificity : Testis specific.

Similarity : Contains 1 RING-type zinc finger.

Contains 4 Tudor domains.

Database links : UniProtKB/Swiss-Prot: Q9BXT8.3

抗體的鑒定：

1）環(huán)指蛋白17抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線(xiàn),計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

NGN3(neurogenin 3; Neurog3) 神經(jīng)元素3抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-APOE 載脂蛋白E抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-ATRIP (Ser68+Ser72) 先關(guān)蛋白TREX1抗體 規(guī)格 0.1ml

山羊抗大鼠 IgG(H+L)/AP 0.1ml 美國(guó)Jackson公司分裝

VPAC2R 英文名稱(chēng)： 腺苷酸環(huán)化酶激活肽受體-II/活性腸肽受體-II抗體 0.1ml

Desmoglein 2 英文名稱(chēng)： 橋粒芯糖蛋白2抗體 0.1ml

Anti-APOE 載脂蛋白E抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-OPCML /FITC 熒光素標(biāo)記樣物質(zhì)結(jié)合蛋白/細(xì)胞粘附分子樣蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rabbit Anti-monkey IgG/HRP 辣根過(guò)氧化物酶標(biāo)記兔抗猴IgGMulti-class antibodies規(guī)格： 0.1ml

人癌胚抗原抗體 Anti-CEA 0.2ml

Rabbit Anti-horse IgM/Cy5 Cy5標(biāo)記的兔抗馬IgM 0.1ml

FEZ1 英文名稱(chēng)： 腦胚胎鋅指樣蛋白1抗體 0.2ml

Rhesus antibody Rh PLAG1 多型性腺瘤基因1蛋白抗體 規(guī)格 0.2ml

Rabbit Anti-monkey IgG/HRP 辣根過(guò)氧化物酶標(biāo)記兔抗猴IgGMulti-class antibodies規(guī)格： 0.1ml

Synaptogyrin 4 英文名稱(chēng)： 神經(jīng)細(xì)胞囊泡循環(huán)蛋白4抗體 0.1ml

E Tag 英文名稱(chēng)： E Tag標(biāo)簽抗體 0.1ml

β-連環(huán)蛋白抗體(β鏈接素) Anti-β catenin/β-cats 0.1ml

Anti-T Beta10/FITC 熒光素標(biāo)記胸腺素β10抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-HP1 alpha (Tyr24) 磷酸化異染色質(zhì)蛋白1抗體(果蠅) 規(guī)格 1ml

MIP-3 beta/ELC/CCL19(Macrophage Inflammatory Protein 3 Beta) 巨噬細(xì)胞炎性蛋白19抗原Multi-class antibodies規(guī)格： 0.5mg

MLF, 小鼠淋巴成纖維細(xì)胞

ACTE1 非洲爪蟾胚胎細(xì)胞

SCGB1A1 Others Human 人 SCGB1A1 / Uteroglobin 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0206SGC7901(人細(xì)胞)5×106cells/瓶×2

人脊髓星形膠質(zhì)細(xì)胞cDNAHA-sp cDNA

Sars結(jié)構(gòu)蛋白表達(dá)株;293sars181A 人膀胱上皮細(xì)胞完全培養(yǎng)基 100mL

CL-0135L1210(小鼠細(xì)胞)5×106cells/瓶×2

CD14 Others Human 人 CD14 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠小腸內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

Pig MSC豬骨髓間質(zhì)干細(xì)胞 Pig MSC of porcine bone marrow mesenchymal stem cells DMEM低糖+10% FBS+glutamin

IL6 Protein Rat 重組大鼠 IL6 / Ierleukin-6 蛋白

RM1(大鼠肌肉成纖維樣細(xì)胞) 5×106cells/瓶×2 PC-12未分化(大鼠腎上腺嗜鉻細(xì)胞瘤)

環(huán)指蛋白17抗體品牌 MLF, 小鼠淋巴成纖維細(xì)胞

ACTE1 非洲爪蟾胚胎細(xì)胞

SCGB1A1 Others Human 人 SCGB1A1 / Uteroglobin 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0206SGC7901(人細(xì)胞)5×106cells/瓶×2

人脊髓星形膠質(zhì)細(xì)胞cDNAHA-sp cDNA

Sars結(jié)構(gòu)蛋白表達(dá)株;293sars181A 人膀胱上皮細(xì)胞完全培養(yǎng)基 100mL