抗體的生物素化標記實驗要點：

1. RAM-11抗體費用 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-Rab27a 

中文名稱   RAM-11抗體費用 

別    名  RAB27A; GS2; HsT18676; MGC117246; RAB27; RAM; Rab27a; 2210402C08Rik; 2410003M20Rik; 4933437C11Rik; ash; RB27A_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Pig, Cow, Horse, Sheep 

產(chǎn)品類型   一抗  

研究領域    轉(zhuǎn)錄調(diào)節(jié)因子 合成與降解 轉(zhuǎn)運蛋白

蛋白分子量  predicted molecular weight: 25kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RAM-11 C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

RAM-11抗體費用 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Summary: The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].

Function : Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Subunit : Binds SYTL1, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5 (By similarity). Binds MLPH and SYTL2. Interacts with UNC13D.

Subcellular Location : Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.

Tissue Specificity : Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.

DISEASE : Griscelli syndrome 2 (GS2) [MIM:607624]: Rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the small GTPase superfamily. Rab family.

Database links : UniProtKB/Swiss-Prot: P51159.3

抗體的鑒定：

1）RAM-11抗體費用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

IL-13(Interleukin-13) 白介素13抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh phospho-CDKN1B/P27kip1 (Ser10) 磷酸化P27抗體/周期素依賴激酶抑制劑 規(guī)格 0.1ml

IgG 干粉 10mg/50mg/100mg 國產(chǎn)

Wnt16 英文名稱： 信號通路Wnt16抗體 0.2ml

Delta 4 英文名稱： Notch信號通路Delta樣配體4抗體 0.1ml

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-PLAUR/uPAR/FITC 熒光素標記尿激酶型纖溶酶原激活因子受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rat IgM (親和純化) 大鼠IgMMulti-class antibodies規(guī)格： 1mg

血紅素氧合酶-1/熱休克蛋白-32抗體 Anti-HO-1/HSP32 0.1ml

Goat Anti-rat IgG 羊抗大鼠IgG 1mg

FoxP3 英文名稱： 叉頭蛋白P3抗體 0.1ml

Rhesus antibody Rh phospho-RelB(Ser552) 磷酸化轉(zhuǎn)錄因子RelB蛋白抗體 規(guī)格 0.1ml

Rat IgM (親和純化) 大鼠IgMMulti-class antibodies規(guī)格： 1mg

Somatostatin Receptor 5 英文名稱： 生長抑素受體5抗體 0.1ml

phospho-MEK1 (Thr292) 英文名稱： 磷酸化原活化蛋白激酶1抗體 0.1ml

CD33抗體(人) Anti-CD33 0.1ml

Anti-VEGF/FITC 熒光素標記VEGF抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh phospho-GSK3 Alpha(Ser21) 磷酸化葡萄糖合成激酶-3α抗體 規(guī)格 0.1ml

E-Selectin E選擇素抗原Multi-class antibodies規(guī)格： 0.5mg

人臍動脈內(nèi)皮細胞

NCI-H345(懸浮) 小細胞

CD83 Others Mouse 小鼠 CD83 / HB15 人細胞裂解液 (陽性對照)

NCI-N87 [N87]人細胞

人胚腎細胞;293 [HEK-293]

人惡性非霍奇金患者的自然殺傷細胞;NK-92MI [NK92MI] 人成纖維細胞完全培養(yǎng)基 100mL

CL-0226SW626(人細胞)5×106cells/瓶×2

FAM19A4 Others Human 人 FAM19A4 / TAFA4 人細胞裂解液 (陽性對照)

人牙齦成纖維細胞cDNAHGF cDNA

豬胚胎傳代細胞;ST 子細胞，SiHa細胞 B22細胞，KM小鼠未分化神經(jīng)瘤株

Sars結(jié)構(gòu)蛋白表達株;293sars181A

HA Others H5N1 甲型 H5N1 血凝素HA1 (Hemagglutinin) 人細胞裂解液 (陽性對照)

RAM-11抗體費用 人臍動脈內(nèi)皮細胞

NCI-H345(懸浮) 小細胞

CD83 Others Mouse 小鼠 CD83 / HB15 人細胞裂解液 (陽性對照)

NCI-N87 [N87]人細胞

人胚腎細胞;293 [HEK-293]

人惡性非霍奇金患者的自然殺傷細胞;NK-92MI [NK92MI] 人成纖維細胞完全培養(yǎng)基 100mL