核糖體蛋白S3抗體說(shuō)明書(shū) 英文名稱  Anti-RPS3

中文名稱  核糖體蛋白S3抗體說(shuō)明書(shū) 

別    名 40S ribosomal protein S3; IMR 90 ribosomal protein S3; Ribosomal protein S3; rps3; RS3_HUMAN; S3.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Zebrafish, Xenopuslaevis,NonPrimates

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 26/54 ribosomal chain dimerkDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RPS3

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

核糖體蛋白S3抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Ribosomal subunits are synthesized in the nucleus, and mature 40S and 60S subunits are exported stoichiometrically into the cytoplasm. Both 40S and 60S subunits are composed of four RNA species and approximately 80 structurally distinct proteins. Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit. Ribosomal proteins have the ability to pass through the nuclear envelope in the native state, making them the largest of the structures accommodated by the nuclear pore complexes. The nuclear export of ribosomal subunits is a unidirectional, saturable and energy-dependent process. Ribosomal Protein S3 a member of the 40S subunit and plays a role in translation and ribosome maturation. Specifically, Ribosomal Protein S3 mediates the formation of the mRNA binding site 3’ of the codon in the decoding site. In addition, Ribosomal Protein S3 is involved in DNA damage recognition as shown by its affinity for abasic sites and 7,8-dihydro-8-oxoguanine residues and its interaction with human base excision repair (BER) proteins OGG1 and Ref-1.

Subunit : Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon.

Subcellular Location : Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Similarity : Belongs to the ribosomal protein S3P family.

Contains 1 KH type-2 domain.

Database links :

UniProtKB/Swiss-Prot: P23396.2

Entrez Gene: 6188 Human

Entrez Gene: 27050 Mouse

Entrez Gene: 140654 Rat

Entrez Gene: 336550 Zebrafish

Omim: 600454 Human

SwissProt: P23396 Human

SwissProt: P62908 Mouse

SwissProt: Q0Z8U2 Pig

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技術(shù)外包服務(wù):

★核糖體蛋白S3抗體說(shuō)明書(shū) 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

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★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。