產(chǎn)品訂購信息：
英文名稱  Anti-RNF7/CKBBP1 

中文名稱   環(huán)指蛋白7抗體品牌 

別    名  CKBBP 1; CKBBP1; CKII beta binding protein 1; CKII beta-binding protein 1; Rbx 2; Rbx2; RBX2_HUMAN; Regulator Of cullins 2; RING box protein 2; Ring finger protein 7; RING-box protein 2; RNF 7; RNF7; ROC 2; ROC2; SAG; Sensitive to apoptosis gene; Sensitive to apoptosis gene protein; Zinc RING finger protein SAG.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 信號轉(zhuǎn)導 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學

蛋白分子量  predicted molecular weight: 13kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RNF7/CKBBP1 (30-75aa) 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

環(huán)指蛋白7抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents. Tissue specificity: Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.

Function : Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

Subunit : Probable part of SCF complexes, which consist of SKP1, CUL1, RNF7/RBX2 and a F-box protein. Interacts (preferentially) with CUL5. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B. Interacts with UBE2F. Interacts with CSNK2B, the interaction is not affected by phosphorylation by CK2.

Subcellular Location : Cytoplasm. Nucleus.

Tissue Specificity : Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.

Post-translational modifications : Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.

Similarity : Belongs to the RING-box family.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q9UBF6.1

GSR/GRase(glutathione reductase) 谷胱甘肽還原酶抗原Multi-class antibodies規(guī)格： 0.5mg

SCARB2 LIMP-2 / SCARB2 / CD36L2 兔單抗 (FITC) Mouse

Rhesus antibody Rh phospho-CREB-1(Ser133) 磷酸化CREB-1抗體 規(guī)格 0.1ml

紅細胞裂解液(10X) 10ml

ZBT24 英文名稱： 鋅指蛋白450抗體 0.2ml

Phospho-Dab1 (Tyr198) 英文名稱： 磷酸化磷脂蛋白受體Dab1抗體 0.1ml

SCARB2 LIMP-2 / SCARB2 / CD36L2 兔單抗 (FITC) Mouse

Anti-PTN/FITC 熒光素標記多效生長因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Streptavidin,SA 鏈霉親和素Multi-class antibodies規(guī)格： 1mg

整合素α4抗體 Anti-ITG-α/ CD49d 0.1ml

名 價 格(RMB)

Fumarate hydratase 英文名稱： 富馬酸水合酶抗體 0.2ml

Rhesus antibody Rh Phospho-PRAS40 (Thr246) 磷酸化蛋白激酶AKT底物1抗體 規(guī)格 0.1ml

Streptavidin,SA 鏈霉親和素Multi-class antibodies規(guī)格： 1mg

SAMD9 英文名稱： SAMD9蛋白抗體 0.2ml

phospho-EphB1 (Tyr928) 英文名稱： 磷酸化酪酸蛋白激酶受體B1抗體 0.1ml

細胞凋亡關(guān)聯(lián)酪氨酸激酶抗體 Anti-AATK 0.1ml

Anti-Wnt3a/FITC 熒光素標記信號通路Wnt3a抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Phospho-FoxO3a (Ser253) 磷酸化叉頭蛋白3A抗體 規(guī)格 0.1ml

CNP/CNPase ( 2',3'-cyclic-nucleotide 3'-phosphodiesterase) C型尿肽抗原Multi-class antibodies規(guī)格： 0.5mg

FLT3LG Protein Mouse 重組小鼠 FLT3L / Flt3 ligand 蛋白 (Fc 標簽)

LA795瘤 肺腺癌

LAIR2 Others Human 人 LAIR2 / CD306 人細胞裂解液 (陽性對照)

SK-OV-3人細胞 Human ovarian cancer cell line SK-OV-3 McCoy'*+10% FBS

大鼠肝細胞瘤;H4-II-E-C3

小鼠骨髓間充質(zhì)肝細胞(MMSC-bm)(5×105) PANC-1, 人*癌細胞 Human

CL-0321BEL-7405(人細胞)5×106cells/瓶×2

MANF Others Mouse 小鼠 MANF / ARMET 人細胞裂解液 (陽性對照)

小鼠肺大內(nèi)皮細胞完全培養(yǎng)基 100mL

LLC1[LL/2]小鼠Lewis細胞 LLC1[LL/2] mice with Lewis lung cancer cells 1640+10% FBS

IL1B Protein Human 重組人 IL-1 beta / IL1B 蛋白 (pro form, His 標簽)

NCI-H716(人結(jié)直腸腺癌細胞) 5×106cells/瓶×2 3T3-L1(小鼠脂肪細胞)

環(huán)指蛋白7抗體品牌 FLT3LG Protein Mouse 重組小鼠 FLT3L / Flt3 ligand 蛋白 (Fc 標簽)

LA795瘤 肺腺癌

LAIR2 Others Human 人 LAIR2 / CD306 人細胞裂解液 (陽性對照)

SK-OV-3人細胞 Human ovarian cancer cell line SK-OV-3 McCoy'*+10% FBS

大鼠肝細胞瘤;H4-II-E-C3

小鼠骨髓間充質(zhì)肝細胞(MMSC-bm)(5×105) PANC-1, 人*癌細胞 Human

抗體的生物素化標記實驗要點：

1. 環(huán)指蛋白7抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）環(huán)指蛋白7抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。