產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-RITA/C12orf52 

中文名稱   12號(hào)染色體開(kāi)放閱讀框52抗體說(shuō)明書 

別    名  C12orf52; Chromosome 12 open reading frame 52; RBPJ-interacting and tubulin-associated protein; RITA; RITA_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Cow, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 29kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RITA/C12orf52 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

12號(hào)染色體開(kāi)放閱讀框52抗體說(shuō)明書 產(chǎn)品應(yīng)用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Noonan syndrome, which includes heart and facial developmental defects among the primary symptoms, is caused by a mutant form of PTPN11 gene product, SH-PTP2. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction. Trisomy 12p leads to facial development defects, seizure disorders and a host of other symptoms varying in severity depending on the extent of mosaicism and is most severe in cases of complete trisomy. The C12orf52 gene product has been provisionally designated C12orf52 pending further characterization.

Function : Tubulin-binding protein that acts as a negative regulator of Notch signaling pathway. Shuttles between the cytoplasm and the nucleus and mediates the nuclear export of RBPJ/RBPSUH, thereby preventing the interaction between RBPJ/RBPSUH and NICD product of Notch proteins (Notch intracellular domain), leading to down-regulate Notch-mediated transcription. May play a role in neurogenesis.

Subunit : Interacts with RBPJ/RBPSUH.

Subcellular Location : Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. Shuttles rapidly between the cytoplasm and the nucleus. The function of centrosome localization is still unclear.

Similarity : Belongs to the RITA family.

Database links : UniProtKB/Swiss-Prot: Q96K30.1

Anti- Beta-Amyloid(1-40)/FITC 熒光素標(biāo)記β淀粉樣肽(1-40)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-K3/FITC 熒光素標(biāo)記抗K3蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CXCL13/BCA1 B-淋巴細(xì)胞趨化因子抗體 規(guī)格 0.1ml

Survivin 細(xì)胞凋亡抑制因子4(抗原) 0.5mg

KIRREL1 英文名稱： 樣蛋白1抗體 0.2ml

Rhesus antibody Rh VSV-G tag VSV-G tag標(biāo)簽抗體 規(guī)格 0.1ml

Anti-K3/FITC 熒光素標(biāo)記抗K3蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-OAT-1/FITC 熒光素標(biāo)記陰離子轉(zhuǎn)運(yùn)蛋白-1(抗人)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Goat Anti-Chicken IgG/HRP 辣根過(guò)氧化物酶標(biāo)記羊抗雞IgGMulti-class antibodies規(guī)格： 0.1ml

胞漿-5′-核苷酸酶-Ⅱ抗體 Anti-CD73 0.2ml

Goat Anti-Bovine IgG/Alexa Fluor 488 Alexa Fluor 488標(biāo)記的羊抗IgG 0.1ml

FLIP delta + gamma 英文名稱： 凋亡調(diào)節(jié)蛋白δ+γ抗體 0.2ml

Rhesus antibody Rh PLC beta 3 磷酯酶Cβ3抗體 規(guī)格 0.1ml

Goat Anti-Chicken IgG/HRP 辣根過(guò)氧化物酶標(biāo)記羊抗雞IgGMulti-class antibodies規(guī)格： 0.1ml

DISC1(disrupted in schizophrenia1)(NT) DISC1 N端抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-CXCR6 細(xì)胞表面趨化因子受體6抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh PBEF (CT) 內(nèi)脂素/前B細(xì)胞克隆增強(qiáng)因子-2(C端抗體) 規(guī)格 0.1ml

SELL Kit Mouse 小鼠 CD62L / L-Selectin / SELL ELISA配對(duì)抗體 ELISA

TAF12 英文名稱： TATA框結(jié)合蛋白關(guān)聯(lián)因子12抗體 0.1ml

C3orf30 英文名稱： 3號(hào)染色體開(kāi)放閱讀框30抗體 0.2ml

Anti-CXCR6 細(xì)胞表面趨化因子受體6抗體Multi-class antibodies規(guī)格： 0.1ml

改良纖維二糖多粘菌素B多粘菌素E(mCPC)瓊脂基礎(chǔ)凍干配套試劑SR0260支添加于50ml025纖維二糖多粘菌素B多粘菌素E(mCPC)瓊脂基礎(chǔ)。

LB Lennox Broth 培養(yǎng)基 250g incubation media LB Lennox Broth 培養(yǎng)基 250g

煙曲霉 支/瓶

桔汁瓊脂250g用于飲料中耐酸細(xì)菌的分離培養(yǎng)

溶血(0.1%)赫氏瓊脂 250g 用于菌分離培養(yǎng)

梭菌屬測(cè)定用培養(yǎng)基250g用于梭菌屬細(xì)菌檢測(cè)

胺藍(lán)-DNA酶瓊脂 Toluidine Blue DNAse Agar 用于核糖核酸酶檢測(cè)

C.L.E.D 培養(yǎng)基 C.L.E.D Medium 250 用于尿液中微生物的選擇性分離培養(yǎng)(OXOID方法)

梭菌增菌培養(yǎng)基250g/瓶用于梭菌的增菌培養(yǎng)incubationmedia梭菌增菌培養(yǎng)基250g/瓶用于梭菌的增菌培養(yǎng)

SimmonsCitrateAgar

12號(hào)染色體開(kāi)放閱讀框52抗體說(shuō)明書 BPLS瓊脂 250(g)  incubation media BPLS瓊脂 250(g)

改良BPLS瓊脂 250(g)  incubation media 改良BPLS瓊脂 250(g)

XLT4瓊脂 250(g)  incubation media XLT4瓊脂 250(g)

MLCB瓊脂 250(g)  incubation media MLCB瓊脂 250(g)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 12號(hào)染色體開(kāi)放閱讀框52抗體說(shuō)明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）12號(hào)染色體開(kāi)放閱讀框52抗體說(shuō)明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。