產(chǎn)品訂購信息：
英文名稱  Anti-RAB3GAP1 

中文名稱   RAB3-GTP酶激活蛋白催化亞單位1抗體品牌 

別    名  DKFZp434A012; KIAA0066; P130; Rab3 GAP; Rab3 GAP p130; RAB3 GTPase activating protein 130 kDa subunit; Rab3 GTPase activating protein catalytic subunit; RAB3GAP; RAB3GAP130; WARBM1; RB3GP_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) G蛋白信號

蛋白分子量  predicted molecular weight: 110kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RAB3GAP1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

RAB3-GTP酶激活蛋白催化亞單位1抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab 3 GAP p130, also known as Rab3 GTPase-activating protein catalytic subunit, is a 981 amino acid protein that belongs to the Rab3-GAP catalytic subunit family. Rab 3 GAP p130 converts active RAB3-GTP to the inactive form RAB3-GDP, and is required for normal eye and brain development. Defects in Rab 3 GAP p130 are the cause of Warburg micro syndrome 1 (WARBM1). WARBM1 is a severe autosomal recessive disorder characterized by developmental abnormalities of the eye and central nervous system and by microgenitalia. The Rab 3 GAP p130 protein may participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. Existing as two alternatively spliced isoforms, the Rab 3 GAP p130 gene is conserved in chimpanzee, dog, cow, mouse, chicken, zebrafish and fruit fly, and maps to human chromosome 2q21.3.

Function : RAB3GAP1 is a member of the RAB3 protein family which are implicated in regulated exocytosis of neurotransmitters and hormones. RAB3GAP, which is involved in regulation of RAB3 activity, is a heterodimeric complex consisting a 130-kD catalytic subunit and a 150-kD noncatalytic subunit. RAB3GAP specifically converts active RAB3-GTP to the inactive form RAB3-GDP (Aligianis et al., 2005 [PubMed 15696165].

Subunit : The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2

Subcellular Location : Cytoplasm. Note=In neurons, it is enriched in the synaptic soluble fraction.

Tissue Specificity : Ubiquitous.

DISEASE : Defects in RAB3GAP1 are the cause of Warburg micro syndrome type 1 (WARBM1) [MIM:600118]. A rare syndrome characterized by microcephaly, microphthalmia, microcornia, congenital cataracts, optic atrophy, cortical dysplasia, in particular corpus callosum hypoplasia, severe mental retardation, spastic diplegia, and hypogonadism.

Similarity : Belongs to the Rab3-GAP catalytic subunit family.

Database links : UniProtKB/Swiss-Prot: Q15042.3

Anti-RAR- Alpha /FITC 熒光素標(biāo)記維受體-α 抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Amyloid Beta 25-35( Beta-Amyloid 25-35) β淀粉樣肽25-35(多肽)Multi-class antibodies規(guī)格： 1mg

瘦素受體抗體 Anti-Leptin receptor 0.2ml

SUR1 英文名稱： 磺酰脲類藥物受體1抗體 0.1ml

FOXL2 英文名稱： 叉頭蛋白L2抗體 0.1ml

Rhesus antibody Rh Phospho-PLC gamma 1/PLCG1(Tyr775) 磷酸化磷酯酶Cγ1抗體 規(guī)格 0.1ml

Amyloid Beta 25-35( Beta-Amyloid 25-35) β淀粉樣肽25-35(多肽)Multi-class antibodies規(guī)格： 1mg

Anti-Phospho-PAK1 (Thr423)/PAK2 (Thr402) /FITC 熒光素標(biāo)記磷酸化p21激活激酶1/2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Goat Anti-Guinea IgG 羊抗豚鼠IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg

γ1氨基受體α1抗體 Anti-GABA-ARα1 0.1ml

Goat Anti-rabbit IgG/Alexa Fluor 647 Alexa Fluor 647標(biāo)記的羊抗兔IgG 0.1ml

FLNC 英文名稱： 細(xì)絲蛋白2抗體 0.1ml

Rhesus antibody Rh Phospho-SRF (Ser103) 磷酸化生長激素釋放因子抗體(血清應(yīng)答因子) 規(guī)格 0.1ml

Goat Anti-Guinea IgG 羊抗豚鼠IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg

PDGF-BB 人血小板衍生生長因子-BBMulti-class antibodies規(guī)格： 48T

Anti-CD155/PVR 病毒受體抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Ninjurin 1 神經(jīng)損傷誘導(dǎo)蛋白1抗體 規(guī)格 0.2ml

Human chaperonin 10,CPN10 ELISA Kit 人伴侶蛋白10 96T

TAB1 英文名稱： 轉(zhuǎn)化生長因子β活化激酶結(jié)合蛋白1抗體 0.1ml

CELSR1 英文名稱： 鈣粘蛋白超家族CELSR1抗體 0.1ml

Anti-CD155/PVR 病毒受體抗體Multi-class antibodies規(guī)格： 0.2ml

產(chǎn)品名稱貨號規(guī)格用途訂購

LB Broth 培養(yǎng)基 250g incubation media LB Broth 培養(yǎng)基 250g

雅致枝霉 支/瓶

最小肉湯培養(yǎng)基250g

AcidL-GMediumBase

十六烷基溴化銨瓊脂250g用于綠膿桿菌的選擇性分離培養(yǎng)(GB標(biāo)準(zhǔn))

假單胞P瓊脂 250(g) incubation media 假單胞P瓊脂 250(g)

彎曲桿菌培養(yǎng)基基礎(chǔ) CCDA Base 250克 彎曲桿菌的分離培養(yǎng)

賴氨酸脫羧酶培養(yǎng)基5生化培養(yǎng)基，賴氨酸脫羧酶試驗(GB、SN標(biāo)準(zhǔn))incubationmedia賴氨酸脫羧酶培養(yǎng)基5生化培養(yǎng)基，賴氨酸脫羧酶試驗(GB、SN標(biāo)準(zhǔn))

EF-18Agar

RAB3-GTP酶激活蛋白催化亞單位1抗體品牌 GN增菌液 1000(g)  incubation media GN增菌液 1000(g)

木糖-賴酸-去氧膽酸鹽培養(yǎng)基(XLD) 250(g)  incubation media 木糖-賴酸-去氧膽酸鹽培養(yǎng)基(XLD) 250(g)

40%尿素水 5mL/支×10(g)  incubation media 40%尿素水 5mL/支×10(g)

WS瓊脂 250(g)  incubation media WS瓊脂 250(g)

抗體的生物素化標(biāo)記實驗要點：

1. RAB3-GTP酶激活蛋白催化亞單位1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）RAB3-GTP酶激活蛋白催化亞單位1抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。