產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) 

中文名稱   磷酸化RNA聚合酶II CTD抗體費(fèi)用 

別    名  p-Rpb1 CTD (Ser2/Ser5); DNA directed RNA polymerase II A; p-Rpb1 CTD(Ser2/Ser5); DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Rabbit 

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    細(xì)胞生物 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 240kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5. 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化RNA聚合酶II CTD抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

Subunit : Belongs to the RNA polymerase beta' chain family.

Subcellular Location : Nucleus.

Anti-CBFA1/RUNX2/FITC 熒光素標(biāo)記成骨特異性轉(zhuǎn)錄因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-HDAC8 /FITC 熒光素標(biāo)記組蛋白去乙酰化酶8抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CEBP-alpha 轉(zhuǎn)錄調(diào)節(jié)因子C/EBP α 抗體 規(guī)格 0.1ml

STAT3(signal transducers and activators of transduction3) 信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3抗原 0.5mg

IRS-3 英文名稱： 受體底物-3抗體 0.2ml

Rhesus antibody Rh TIAM1 T侵襲轉(zhuǎn)移誘導(dǎo)蛋白1抗體 規(guī)格 0.1ml

Anti-HDAC8 /FITC 熒光素標(biāo)記組蛋白去乙酰化酶8抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-CD4/HRP 辣根過(guò)氧化物酶標(biāo)記CD4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Mink IgG 水貂IgG (親和純化)Multi-class antibodies規(guī)格： 1mg

人類白細(xì)胞抗原E Anti-HLA-E 0.1ml

Rabbit Anti-Avidin 兔抗親和素 1mg

FoxP1 英文名稱： 叉頭蛋白P1抗體 0.2ml

Rhesus antibody Rh phospho-Ret/HSCR1(Tyr1062) 磷酸化原癌基因酪氨酸蛋白激酶受體RET抗體 規(guī)格 0.1ml

Mink IgG 水貂IgG (親和純化)Multi-class antibodies規(guī)格： 1mg

CNTF 大鼠睫狀神經(jīng)營(yíng)養(yǎng)因子Multi-class antibodies規(guī)格： 48T

Anti-Endostatin 內(nèi)皮抑素/內(nèi)皮他丁抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MVD 甲羥脫羧酶抗體 規(guī)格 0.1ml

TX-A2(Human Thromboxane A2) ELISA Kit 人素A2 96T

ST5 英文名稱： 蛋白5抗體 0.2ml

C1orf187 英文名稱： 1號(hào)染色體開(kāi)放閱讀框187抗體 0.2ml

Anti-Endostatin 內(nèi)皮抑素/內(nèi)皮他丁抗體Multi-class antibodies規(guī)格： 0.1ml

TMP瓊脂培養(yǎng)基250g用于金黃色葡萄球菌的分離培養(yǎng)

HelicobacterPyloriMedium

營(yíng)養(yǎng)肉湯 (NB) Nutrient Broth 250 一般細(xì)菌培養(yǎng)、轉(zhuǎn)種、復(fù)壯、增菌等(GB標(biāo)準(zhǔn))

馬丁瓊脂250g/瓶用于多殺性巴桿菌、豬菌、、鏈球菌菌苗 及檢驗(yàn)incubationmedia馬丁瓊脂250g/瓶用于多殺性巴桿菌、豬菌、、鏈球菌菌苗 及檢驗(yàn)

含0.6%酵母膏的胰酪胨大豆肉湯(TSB-YE) 300ml/袋*10 用于單增李氏菌增菌培養(yǎng)(SN、GB標(biāo)準(zhǔn))

AntibioticAgarNO.4

抗生素檢定培養(yǎng)基2號(hào)(pH 7.9±0.1) 250(g) incubation media 抗生素檢定培養(yǎng)基2號(hào)(pH 7.9±0.1) 250(g)

麥芽汁瓊脂培養(yǎng)基 250g 供酵母菌的培養(yǎng)、鑒定及保存菌種用。

原材料系列incubationmedia原材料系列

SulfiteReducingAnaerobesSporeLiquidMedium

磷酸化RNA聚合酶II CTD抗體費(fèi)用 蛋白胨(發(fā)酵原料)  規(guī)格：  1kg  用途：  發(fā)酵原材料

牛肉浸粉  規(guī)格：  400g  用途：  培養(yǎng)基原材料

牛肉浸膏  規(guī)格：  30Kg  用途：  培養(yǎng)基原材料

牛肉浸膏  規(guī)格：  5Kg  用途：  培養(yǎng)基原材料

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化RNA聚合酶II CTD抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸化RNA聚合酶II CTD抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。