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視網(wǎng)膜母細(xì)胞瘤結(jié)合蛋白6抗體說明書 英文名稱  Anti-RBBP6

中文名稱  視網(wǎng)膜母細(xì)胞瘤結(jié)合蛋白6抗體說明書 

別    名 Retinoblastoma binding protein 6; RBBP6; MY038; P2P R; P2PR; p53 associated cellular protein of testis; PACT;Proliferationpotentialrelated protein; Protein P2P R; Protein P2PR; Protein RBQ 1; Protein RBQ1; RB binding Q protein 1; RBBP 6; RBQ 1; RBQ1; Retinoblastoma binding protein 6 isoform 3; Retinoblastoma binding Q protein 1; SNAMA; RBBP6_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 細(xì)胞凋亡 細(xì)胞周期蛋白 細(xì)胞生物標(biāo)志物

蛋白分子量  predicted molecular weight: 201kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RBBP6 (1265-1315aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

視網(wǎng)膜母細(xì)胞瘤結(jié)合蛋白6抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. Retinoblastoma binding protein 6 binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene.

Function : E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.

Subunit : Interacts with p53/TP53 and RB1 (By similarity). Interacts also with MDM2 and YBX1. Interacts with NEK6.

Subcellular Location : ucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.

Tissue Specificity : Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by NEK6.

Similarity : Contains 1 CCHC-type zinc finger.

Contains 1 DWNN domain.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q7Z6E9.1

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