產(chǎn)品訂購信息：
英文名稱  Anti-RING2/RNF2 

中文名稱   環(huán)指蛋白2抗體說明書 

別    名  BAP 1; BAP1; DING; DinG protein; E3 ubiquitin protein ligase RING 2; E3 ubiquitin protein ligase RING2; E3 ubiquitin-protein ligase RING2; HIP2 interacting protein 3; HIP2-interacting protein 3; HIPI 3; HIPI3; Huntingtin interacting protein 2 interacting protein 3; Huntingtin-interacting protein 2-interacting protein 3; Polycomb M33 interacting protein Ring 1B; Polycomb M33 interacting protein Ring1B; Protein DinG; RING 1B; RING 2; Ring finger protein 1b; Ring finger protein 2; RING finger protein BAP 1; RING finger protein BAP-1; RING finger protein BAP1; RING1b; RING2_HUMAN; RNF 2; RNF2.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué)

蛋白分子量  predicted molecular weight: 37kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RING2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

環(huán)指蛋白2抗體說明書 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008].

Function : E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.

Subunit : Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of a PRC1-like complex. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. Interacts with Interacts with PCGF2, CBX4, CBX6, CBX7 and CBX8. Interacts with CBX2, BMI and PHC2. Interacts with RYBP, HIP2 and TFCP2.

Subcellular Location : Nucleus. Chromosome. Note=Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.

Post-translational modifications : Polyubiquitinated in the presence of UBE2D3 (in vitro).

Monoubiquitinated, by auto-ubiquitination.

Similarity : Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q99496.1

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Rhesus antibody Rh HO-1 血紅素氧合酶 1/熱休克蛋白32抗體 規(guī)格 0.1ml

sP-selectin ELISA Kit 大鼠可溶性P選擇素 96T

PLEKHA7 英文名稱： PLEKHA7蛋白抗體 0.1ml

c21orf63 英文名稱： 21號染色體開放閱讀框63抗體 0.2ml

Anti-PAX9 配對盒基因9抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-SIGLEC10/SLG2/FITC 熒光素標(biāo)記唾液酸結(jié)合性免疫球蛋白樣凝集素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-CD93/C1qrp/FITC 熒光素標(biāo)記CD93抗體IgGMulti-class antibodies規(guī)格： 0.2ml

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Goat Anti-Chicken IgG/Cy3 Cy3標(biāo)記的羊抗雞IgG 0.1ml

FUZ/FUZZY 英文名稱： 眼睛和頭發(fā)顏色相關(guān)蛋白FUZ抗體 0.2ml

Rhesus antibody Rh PPAPDC3 磷脂酸磷酸酶2結(jié)構(gòu)域3抗體 規(guī)格 0.2ml

Anti-CD93/C1qrp/FITC 熒光素標(biāo)記CD93抗體IgGMulti-class antibodies規(guī)格： 0.2ml

mouse adiponectin 小鼠脂聯(lián)素Multi-class antibodies規(guī)格： 48T

Anti-ET-1(whole serum) 內(nèi)皮素-1抗體(抗血清)Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MRPS4/IMP3 線粒體核糖體蛋白S4抗體 規(guī)格 0.2ml

ABP(Human Androgen Binding Protein) ELISA Kit 人雄激素結(jié)合蛋白 96T

SNF2L 英文名稱： 解旋酶SNF2L抗體 0.2ml

phospho-cardiac Troponin I (Thr143) 英文名稱： 磷酸化心肌肌鈣蛋白抗體 0.2ml

Anti-ET-1(whole serum) 內(nèi)皮素-1抗體(抗血清)Multi-class antibodies規(guī)格： 0.1ml

炭疽病診斷血清(陰性)疽桿菌檢測用配套試劑

ENDO agar ENDO氯脂 1.04044.0504 MERCK默克 incubation media ENDO agar ENDO氯脂 1.04044.0504 MERCK默克

溴紫葡萄糖蛋白胨水培養(yǎng)基 250g 用于壓力蒸汽滅菌效果評價(GB15981-1995和GB15979-2002)。

F344大鼠骨髓間質(zhì)干細(xì)胞成軟骨誘導(dǎo)分化完全培養(yǎng)基F344ratbonemarrowmesenchymalstemcellsintochondrocytesinduceddifferentiationofcompletemedium

FB1additive(A、B)

Xylose-gelatinmedium

亮綠乳糖培養(yǎng)基 Brilliant Green Lactose Medium 用于飲用天然礦泉水中大腸桿菌的檢驗(GB標(biāo)準(zhǔn))

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MUG

環(huán)指蛋白2抗體說明書 馬鈴薯瓊脂  規(guī)格：  100g  用途：  用于霉菌的培養(yǎng)

馬鈴薯葡萄糖瓊脂(PDA培養(yǎng)基)  規(guī)格：  250g  用途：  供霉菌和酵母菌計數(shù)及分離培養(yǎng)

改良馬丁瓊脂培養(yǎng)基(真菌營養(yǎng)瓊脂培養(yǎng)基)  規(guī)格：  250g  用途：  用于真菌培養(yǎng)及藥品和生物制品無菌檢查用

改良馬丁培養(yǎng)基 (真菌培養(yǎng)基)  規(guī)格：  250g  用途：  用于真菌培養(yǎng)及藥品和生物制品無菌檢查用。

抗體的生物素化標(biāo)記實驗要點：

1. 環(huán)指蛋白2抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）環(huán)指蛋白2抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。