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★磷酸化RhoA蛋白抗體費用 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

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★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

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實驗流程：

磷酸化RhoA蛋白抗體費用 英文名稱  Anti-Phospho-RhoA(Ser188)

中文名稱  磷酸化RhoA蛋白抗體費用 

別    名 RHOA(phospho S188); RhoA protein; Aplysia ras related homolog 12; ARH 12; ARH12; ARHA; H 12; Oncogene RHO H12; Ras homolog gene family member A; RHO 12; Rho A; RHO H12; RHO12; RHOH 12; RHOH12; Small GTP binding protein Rho A; Small GTP binding protein RhoA; Transforming protein Rho A; Transforming protein RhoA.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Chicken, Dog, Cow, Sheep

產(chǎn)品類型   一抗 磷酸化抗體  

研究領域    免疫學 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 21kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human RhoA around the phosphorylation site of Ser188

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化RhoA蛋白抗體費用 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Subunit : nteracts with RGNEF. Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2.

Similarity : Belongs to the small GTPase superfamily. Rho family.

RhoA蛋白主要分布在細胞質(zhì)和細胞膜,RhoA蛋白家族是控制細胞骨架蛋白組合、調(diào)節(jié)基因表達等細胞活動過程中的關鍵信號轉(zhuǎn)導成分。通常,RhoA通過激活其下游的Rho 連接激酶(Rho associated kinase,ROK)而起作用。在調(diào)節(jié)細胞骨架組建的過程中,RhoA與其作用對象均在胞質(zhì)內(nèi)。

全、新、優(yōu)、品、好四大特點：

磷酸化RhoA蛋白抗體費用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

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