產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-RASA1 

中文名稱   Rho GTP酶激活蛋白1/骨肥大綜合征相關(guān)蛋白抗體品牌 

別    名  Ras GAP; CM AVM; CMAVM; DKFZp434N071; GAP; GTPase activating protein; GTPase-activating protein; OTTHUMP00000222390; OTTHUMP00000222391; OTTHUMP00000222392; OTTHUMP00000222393; p120GAP; p120RASGAP; PKWS; Ras GTPase-activating protein 1; RAS p21 protein activator (GTPase activating protein) 1; Ras p21 protein activator; RASA; RASA1; RASA1_HUMAN; RasGAP; Triphosphatase activating protein.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號(hào)

蛋白分子量  predicted molecular weight: 116kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Ras GTPase-activating protein 1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

Rho GTP酶激活蛋白1/骨肥大綜合征相關(guān)蛋白抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode ubiquitously expressed proteins (1,2). p21Ras can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (3). Oncogenic p21Ras proteins are trapped in the excited signal-emitting state because the mechanism normally employed to delimit their excitation period, hydrolysis of their bound GTP to GDP, is impaired as a result of specific mutations (3). Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold (4,5). The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity (6,7), and proteins that stimulate the GTPase activity of three other low molecular weight GTPases, including Rho, Rab 3A and Rap 1, have also been described (8,9).

Function : Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

Subunit : Interacts with SQSTM1. Interacts with SPSB1; the interaction does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1.

Subcellular Location : Cytoplasm.

Tissue Specificity : In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).

DISEASE : Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.

Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.

Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.

Similarity : Contains 1 C2 domain.

Contains 1 PH domain.

Contains 1 Ras-GAP domain.

Contains 2 SH2 domains.

Contains 1 SH3 domain.

Database links : UniProtKB/Swiss-Prot: P20936.1

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CD19(B-lymphocyte Anti-gen CD19 precursor) CD19(抗原)Multi-class antibodies規(guī)格： 0.5mg

Anti-Insulin/FITC 熒光素標(biāo)記標(biāo)記胰島素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-RUNX1/AML1/FITC 熒光素標(biāo)記兔抗人、大、小鼠急性髓細(xì)胞1蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ANKRD53 錨蛋白重復(fù)域53抗體 規(guī)格 0.2ml

Rabbit Anti-mouse IgG/Cy7 Cy7標(biāo)記的兔抗小鼠IgG 0.1ml

G protein alpha S 英文名稱： G蛋白αS抗體(鳥(niǎo)嘌呤核苷酸結(jié)合蛋白Gα s) 0.2ml

Rhesus antibody Rh Rabbit Anti-human IgG F(ab')2/PE-Cy5.5 PE-Cy5.5標(biāo)記的兔抗人IgG F(ab')2 規(guī)格 0.1ml

Anti-RUNX1/AML1/FITC 熒光素標(biāo)記兔抗人、大、小鼠急性髓細(xì)胞1蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

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Anti-DLK1 穿膜蛋白DLK1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh NDRG2 基因NDRG2抗體 規(guī)格 0.2ml

Human leukemia inhibitory factor receptor,LIFR ELISA Kit 人抑制因子受體 96T

Slc4a7 英文名稱： 碳酸氫協(xié)同轉(zhuǎn)運(yùn)蛋白4-A7抗體 0.2ml

CD93 英文名稱： CD93抗體 0.2ml

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PPLOAgar

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哥倫比亞培養(yǎng)基 250g 用于多種微生物的增菌培養(yǎng)

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GN增菌液 250g 用于革蘭氏陰性菌的選擇性培養(yǎng)，特別是志賀氏菌和沙門(mén)氏菌的增菌培養(yǎng)(GB/T4789.28-2003 中4.17，...

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CIN-1添加劑 1ml*5 每支添加于200ml

Rho GTP酶激活蛋白1/骨肥大綜合征相關(guān)蛋白抗體品牌 玫瑰紅瓊脂培養(yǎng)基  規(guī)格：  250g  用途：  供藥品和生物制品中霉菌和酵母菌的計(jì)數(shù)、分離和培養(yǎng)用。（《中華人民共和國(guó)藥典》2010年版）

Fraser肉湯增菌液（FB1，F(xiàn)B2）基礎(chǔ)  規(guī)格：  250g  用途：  用于李斯特氏的選擇性增菌培養(yǎng)（SN/T0184.1-2005，ISO標(biāo)準(zhǔn)）。

PALCAM瓊脂基礎(chǔ)  規(guī)格：  250g  用途：  用于單增李斯特氏菌的選擇性分離培養(yǎng)（GB 4789.30-2010和SN/T0184-2005）。

牛津瓊脂基礎(chǔ)  規(guī)格：  100g  用途：  用于單核增生李斯特氏菌的選擇性分離(SN0184-2005)。

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. Rho GTP酶激活蛋白1/骨肥大綜合征相關(guān)蛋白抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）Rho GTP酶激活蛋白1/骨肥大綜合征相關(guān)蛋白抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。