增殖細(xì)胞核抗原抗體說明書 英文名稱  Anti-PCNA-Proliferation Marker

中文名稱  增殖細(xì)胞核抗原抗體說明書 

別    名 Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen; PCNA_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞類型標(biāo)志物

蛋白分子量  predicted molecular weight: 29kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PCNA C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

增殖細(xì)胞核抗原抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Proliferation Marker

Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

Function : Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Subunit : Homotrimer (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A (By similarity). Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with PARPBP. Interacts (when ubiquitinated) with SPRTN; leading to enhance RAD18-mediated PCNA ubiquitination. Interacts (when polyubiquitinated) with ZRANB3. Interacts with SMARCAD1. Interacts with CDKN1C. Interacts with KIAA0101/PAF15 (via PIP-box).

Post-translational modifications : Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.

Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.

Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.

Similarity : Belongs to the PCNA family.

Database links : Belongs to the PCNA family.

PCNA是一種僅在增殖細(xì)胞中合成或表達(dá)的核內(nèi)多肽，其表達(dá)和合成與細(xì)胞周期有關(guān)。主要表達(dá)于增殖細(xì)胞的S期、G1期和G2初期。

PCNA主要作為判斷各種(包括胃腸道癌腫、、、等)細(xì)胞增殖和其惡性程度的一種指標(biāo).

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

增殖細(xì)胞核抗原抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

Anti-VIP/FITC 熒光素標(biāo)記活性腸肽抗體蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

ChRM3 peptide 毒蕈堿型乙酰膽堿受體M3抗原Multi-class antibodies規(guī)格： 0.5mg

細(xì)胞毒性受體NK-p44抗體 Anti-CD336/NKp44/NCR2 0.1ml

SOX22 英文名稱： 核轉(zhuǎn)錄因子SOX22抗體 0.2ml

ERp19 英文名稱： 內(nèi)質(zhì)網(wǎng)蛋白ERp19抗體 0.2ml

Rhesus antibody Rh phospho-GABBR2 (Ser892) 磷酸化G氨基B型受體2抗體 規(guī)格 0.1ml

ChRM3 peptide 毒蕈堿型乙酰膽堿受體M3抗原Multi-class antibodies規(guī)格： 0.5mg

Anti-LOX-1/OLR1/FITC 熒光素標(biāo)記凝集素樣氧化型受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti- Alpha-Synuclein/FITC 熒光素標(biāo)記核突觸蛋白-α抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Adiponectin receptor 2 脂聯(lián)素受體2抗體 規(guī)格 0.1ml

Rabbit Anti-Goat IgG/Alexa Fluor 647 Alexa Fluor 647標(biāo)記的兔抗羊IgG 0.1ml

GABRA2 英文名稱： G基A型受體α2/GABAA Rα2抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-chicken IgM/Gold 膠體金標(biāo)記的兔抗雞IgM 規(guī)格 2ml

Anti- Alpha-Synuclein/FITC 熒光素標(biāo)記核突觸蛋白-α抗體IgGMulti-class antibodies規(guī)格： 0.2ml

P53 大鼠P53蛋白Multi-class antibodies規(guī)格： 48T

Anti-Phospho-EGFR(Tyr1173) 磷酸化表皮生長因子受體抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MYLK3 肌球蛋白輕鏈激酶3抗體 規(guī)格 0.2ml

IgHV(Human immunoglobulin heavy chain variable region) ELISA Kit 人免疫球蛋白重鏈可變區(qū) 96T

SIGLEC10 英文名稱： 唾液酸結(jié)合性免疫球蛋白樣凝集素抗體 0.2ml

C21orf33 英文名稱： 21號染色體開放閱讀框33抗體 0.2ml

Anti-Phospho-EGFR(Tyr1173) 磷酸化表皮生長因子受體抗體Multi-class antibodies規(guī)格： 0.1ml

AK瓊脂(2號)250g用于檢測牛奶和乳制品中抗生素殘留時(shí)孢子液制備

Demi-Fraser Broth Base incubation media Demi-Fraser Broth Base

比基尼鏈霉菌 支/瓶

含225mlLB袋用于李斯特氏菌制樣和前增菌培養(yǎng)(GB標(biāo)準(zhǔn))

YPD瓊脂 250g 用于測定酵母菌總數(shù)(GB標(biāo)準(zhǔn))

皰肉牛肉粒加入皰肉基礎(chǔ)中，配成皰肉培養(yǎng)基

錳鹽營養(yǎng)瓊脂 Mn2+Nutrient Agar 用于嗜熱需氧芽孢桿菌的檢驗(yàn)

四環(huán)素檢定瓊脂 Tetracyline Examination Agar 250克 副溶血性弧菌的選擇性分離培養(yǎng)

Andrade氏糖類肉湯250用于細(xì)菌的糖發(fā)酵試驗(yàn)incubationmediaAndrade氏糖類肉湯250用于細(xì)菌的糖發(fā)酵試驗(yàn)

磷酸鹽緩沖液(pH7.2) 250g 用于樣品的稀釋

增殖細(xì)胞核抗原抗體說明書 胰蛋白胨大豆瓊脂 (TSA) （大豆酪蛋白瓊脂培養(yǎng)基）  規(guī)格：  250g  用途：  用于普通的或營養(yǎng)要求較高的細(xì)菌的培養(yǎng)以及阪崎腸桿菌的培養(yǎng)，還用于醫(yī)藥工業(yè)潔凈室無菌程度的監(jiān)...

胰酪胨大豆瓊脂培養(yǎng)基  規(guī)格：  250g  用途：  一般細(xì)菌的培養(yǎng)，藥品、生物制品的無菌試驗(yàn)以及抑菌劑效力檢查。（CP）

營養(yǎng)瓊脂培養(yǎng)基  規(guī)格：  250g  用途：  供細(xì)菌總數(shù)測定、保存菌種及純培養(yǎng)用。(CP)

營養(yǎng)肉湯培養(yǎng)基  規(guī)格：  250g  用途：  一般細(xì)菌培養(yǎng)、轉(zhuǎn)種、復(fù)壯、增菌等。(CP)

技術(shù)外包服務(wù):

★增殖細(xì)胞核抗原抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。