技術外包服務:

★優(yōu)先表達抗原抗體規(guī)格 分子生物學：質粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構建等

★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構建、細胞RNAi技術等等。

實驗流程：

優(yōu)先表達抗原抗體規(guī)格 英文名稱  Anti-PRAME

中文名稱  優(yōu)先表達抗原抗體規(guī)格 

別    名 MAPE; Melanoma antigen preferentially expressed in tumors; OIP 4; OIP-4; OIP4; OPA interacting protein 4; Opa interacting protein OIP4; OPA-interacting protein 4; PRAME; PRAME_HUMAN; Preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma; RP23-250F8.3.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應   Human

產(chǎn)品類型   一抗  

研究領域     細胞生物 信號轉導 細胞生物標志物 表觀遺傳學

蛋白分子量  predicted molecular weight: 56kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PRAME (174-210aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

優(yōu)先表達抗原抗體規(guī)格 產(chǎn)品應用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008].

Function : Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.

Subunit : Interacts with RARA (via the ligand-binding domain); the interaction is direct and ligand (retinoic acid)-dependent. Interacts with EZH2; required to repress RAR signaling.

Subcellular Location : Nucleus (Probable). Cell membrane.

Similarity : Belongs to the PRAME family.

Contains 4 LRR (leucine-rich) repeats.

全、新、優(yōu)、品、好四大特點：

優(yōu)先表達抗原抗體規(guī)格 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質量好，投訴比較少。

好：我公司具有優(yōu)質的技術團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

正在熱銷的相關產(chǎn)品：

Anti-CD2AP/FITC 熒光素標記白細胞分化抗原CD2AP抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-Histone H3 (Thr11)/FITC 熒光素標記磷酸化組蛋白3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CDH11/OB-Cadherin 鈣粘附蛋白-11抗體 規(guī)格 0.1ml

S-100A1 (S100 calcium-binding protein A1) 鈣結合蛋白S100A1抗原 0.5mg

IGSF3 英文名稱： 免疫球蛋白超家族成員3抗體 0.2ml

Rhesus antibody Rh TFEB T淋巴細胞轉錄調節(jié)因子TFEB抗體 規(guī)格 0.1ml

Anti-Phospho-Histone H3 (Thr11)/FITC 熒光素標記磷酸化組蛋白3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Ext-N(Human N-terminal extein) ELISA Kit 人N端外顯肽Multi-class antibodies規(guī)格： 48T

Anti-Phospho-PLC gamma 1 (Tyr783) 磷酸化磷酯酶Cγ1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh HHV8/ORF K14 人類病毒8 ORF14抗體 規(guī)格 0.2ml

IP-10/CXCL10 ELISA Kit 大鼠干擾素誘導蛋白10 96T

PRX 英文名稱： 軸周蛋白PRX抗體 0.1ml

C9orf66 英文名稱： 9號染色體開放閱讀框66抗體 0.2ml

Anti-Phospho-PLC gamma 1 (Tyr783) 磷酸化磷酯酶Cγ1抗體Multi-class antibodies規(guī)格： 0.1ml

Eotaxin 1/CCL11(Mouse Eotaxin 1) ELISA Kit 小鼠嗜酸粒細胞趨化蛋白Multi-class antibodies規(guī)格： 48T

Anti-CAP2/FITC 熒光素標記環(huán)化酶相關蛋白CAP-2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh EMR1 表皮生長因子樣激素受體1 規(guī)格 0.2ml

ANG- II (Rat angiotension II ) ELISA Kit 大鼠緊張素Ⅱ 96T

phospho-MEK5(Ser137) 英文名稱： 磷酸化原活化蛋白激酶激酶5抗體 0.1ml

ATG18 英文名稱： 自噬相關蛋白18抗體 0.2ml

Anti-CAP2/FITC 熒光素標記環(huán)化酶相關蛋白CAP-2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

615小鼠T細胞性瘤株;L7712

MAP2K6 Others Human 人 MKK6 / MEK6 / MAP2K6 (207 Ser/Asp, 211 Thr/Asp) 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

CM-M017小鼠*導管上皮細胞完全培養(yǎng)基100mL

C6, 大鼠腦細胞 導管癌細胞,ZR-75-1細胞 J-1111(單核細胞)

小鼠細胞;EL4

REN Others Mouse 小鼠 REN1 / Renin-1 人細胞裂解液 (陽性對照)

615小鼠T細胞性瘤株;L7712

MAP2K6 Others Human 人 MKK6 / MEK6 / MAP2K6 (207 Ser/Asp, 211 Thr/Asp) 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

CM-M017小鼠*導管上皮細胞完全培養(yǎng)基100mL

C6, 大鼠腦細胞 導管癌細胞,ZR-75-1細胞 J-1111(單核細胞)

小鼠細胞;EL4

REN Others Mouse 小鼠 REN1 / Renin-1 人細胞裂解液 (陽性對照)

優(yōu)先表達抗原抗體規(guī)格 FGFR4 Others Rat 大鼠 FGFR4 / FGF Receptor 4 人細胞裂解液 (陽性對照)

CM-R004大鼠氣管上皮細胞完全培養(yǎng)基100mL

小鼠內臟脂肪細胞完全培養(yǎng)基 100mL

THP1-Blue-CD14(穩(wěn)定株)人單核細胞 THP1-Blue-CD14 (stable sain) in human monocytes 1640+10% FBS(熱滅活)+200ug/ml Zeocin+10ug/ml Blasticidin S+0.05mM β-ME

IL17A Protein Canine 重組狗 IL17 / IL17A 蛋白

人神經(jīng)細胞(HN) (10×106 ) 小鼠骨髓間充質干細胞(EGFP標記) MGC80-3(MGC-803)人細胞