產(chǎn)品訂購信息：
英文名稱  Anti-phospholamban 

中文名稱   受磷蛋白/磷蛋白抗體說明書 

別    名  Cardiac phospholamban; CMD1P; PLB; PLN; PPLA_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow 

產(chǎn)品類型   一抗  

研究領(lǐng)域     心 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 通道蛋白 線粒體 細(xì)胞膜蛋白

蛋白分子量  predicted molecular weight: 5.7kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human phospholamban 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

受磷蛋白/磷蛋白抗體說明書 產(chǎn)品應(yīng)用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The Sarco(endo)plasmic-reticulum (SER) regulatory protein, Phospholamban (PLB), is a small, plasma membrane-associated phospho-protein found in the SER of cardiac, smooth and slow-twitch muscle. Believed to assemble into a pentamer, PLB regulates cardiac contractility and Ca2+ affinity for cardiac SER Ca2+ ATPase (SERCA2a). Non-phosphorylated PLB associates with SERCA2a, and inhibits Ca2+ reuptake into the SER. PLB activation occurs when key Serine/Threonine residues in PLB (Ser-10, Ser-16, Thr-17) are phosphorylated by numerous effectors, which include PKC, PKA, PKG, and CaM kinase. Phosphorylation of PLB causes dissociation from SERCA2a and a subsequent increase in the rate of Ca2+ reuptake into the SER, which accelerates ventricular relaxation.

Function : Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.

Subcellular Location : Mitochondrion membrane; Single-pass membrane protein. Sarcoplasmic reticulum.

Tissue Specificity : Heart.

DISEASE : Defects in PLN are the cause of cardiomyopathy dilated type 1P (CMD1P) [MIM:609909]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Defects in PLN are the cause of familial hypertrophic cardiomyopathy type 18 (CMH18) [MIM:613874]. CMH18 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Similarity : Belongs to the phospholamban family.

Database links : UniProtKB/Swiss-Prot: P26678.1

Anti-Caspase-13/FITC 熒光素標(biāo)記半胱胺酸蛋白酶蛋白-13抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-HCFHrp/BTA/FITC 熒光素標(biāo)記抗膀胱抗原IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CEP55 中心體蛋白55kDa抗體 規(guī)格 0.2ml

TLR4/CD284 (Toll-like receptor 4) Toll樣受體4抗原 0.5mg

IGF 1 英文名稱： 樣生長因子1抗體 0.1ml

Rhesus antibody Rh TKTL1 酮基移換酶樣蛋白1抗體 規(guī)格 0.2ml

Anti-HCFHrp/BTA/FITC 熒光素標(biāo)記抗膀胱抗原IgGMulti-class antibodies規(guī)格： 0.2ml

FPA(Human fibrinopeptide A) ELISA Kit 人血纖肽/纖維蛋白肽AMulti-class antibodies規(guī)格： 48T

Anti-Phospho-Rb (Ser608) 磷酸化視網(wǎng)膜母細(xì)胞瘤相關(guān)蛋白1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh H2N2 Hemagglutinin 甲型病毒血凝素抗體(H2N2) 規(guī)格 0.2ml

iNOS ELISA KIT 大鼠誘導(dǎo)型合成酶 96T

OMPC 英文名稱： 大腸桿菌外膜孔道蛋白C抗體 0.1ml

BTNL3 英文名稱： 嗜乳脂蛋白樣3抗體 0.2ml

Anti-Phospho-Rb (Ser608) 磷酸化視網(wǎng)膜母細(xì)胞瘤相關(guān)蛋白1抗體Multi-class antibodies規(guī)格： 0.1ml

LN ELISA Kit 大鼠層連蛋白/板層素Multi-class antibodies規(guī)格： 48T

Anti-Bad/FITC 熒光素標(biāo)記相關(guān)死亡促進(jìn)因子Bad抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Ets1/ETS-1 Ets轉(zhuǎn)錄因子家族ets1抗體 規(guī)格 0.1ml

Plant 25-Dihydroxy vitamin D3,HVD3 ELISA Kit 植物25羥基維生素D3 96T

Phospho-MYPT1(Thr696) 英文名稱： 磷酸化肌球蛋酸酶抗體 0.1ml

Angiopoietin-1 英文名稱： 生成素-1抗體 0.1ml

Anti-Bad/FITC 熒光素標(biāo)記相關(guān)死亡促進(jìn)因子Bad抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CL-0012A172(人腦細(xì)胞)5×106cells/瓶×2

ART3 Others Human 人 ART3 人細(xì)胞裂解液 (陽性對照)

心肌細(xì)胞生長添加物CMGS

ISK (Ishikawa)細(xì)胞，人細(xì)胞 小鼠小細(xì)胞,Bv-2細(xì)胞 心肌成纖維細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

GH3(大鼠細(xì)胞) 5×106cells/瓶×2

HOB Pellet 人成骨細(xì)胞團(tuán)塊 > 1 mio.cells 人脈絡(luò)叢內(nèi)皮細(xì)胞總RNAHCPEC NA

CL-0010786-O(人腎透明細(xì)胞癌細(xì)胞)5×106cells/瓶×2

IFNG Others Human 人 IFN-gamma / IFNG / γ-IFN CHO細(xì)胞裂解液 (陽性對照)

肝細(xì)胞生長添加物HGS

牦牛皮膚細(xì)胞;BMU-S1 單核細(xì)胞巨噬細(xì)胞，J774A1細(xì)胞 Raji(Butt's 細(xì)胞)

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KM9803

JAG1 Others Human 人 JAG1 / JAGL1 / CD339 人細(xì)胞裂解液 (陽性對照)

受磷蛋白/磷蛋白抗體說明書 人表皮淋巴內(nèi)管皮細(xì)胞(HDLEC)(5×105)

人非小細(xì)胞;NCI-H2087

HUVEC-12, 人臍內(nèi)皮細(xì)胞系

人Burkkit細(xì)胞;Daudi 大鼠腎小球內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

MMQ 小鼠細(xì)胞

FETUB Others Human 人 Fetuin-B / FETUB 人細(xì)胞裂解液 (陽性對照)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 受磷蛋白/磷蛋白抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）受磷蛋白/磷蛋白抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。