中文名稱  絲束蛋白T Plastin抗體說明書 

英文名稱  Anti-PLASTIN3/T Plastin

別    名 Plastin 3 (T isoform); PLS3; PLS 3; PLS-3; Plastin 3; Plastin3; Plastin-3; T fimbrin.

產(chǎn)品屬性：

絲束蛋白T Plastin抗體說明書 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 細(xì)胞分化

蛋白分子量  predicted molecular weight: 69kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PLASTIN3(412-459aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

絲束蛋白T Plastin抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 T Plastin is a representative of an important family of actin-cross-linking and -bundling proteins that organize most of the actin networks in cells. T Plastin is associated with the actin core bundle of microvilli, stereocilia and microspikes in epithelial cells and was proposed to participate in the assembly of these structures. In mesenchymal cells, T plastin localizes to focal adhesions and the leading edge, a broad lamellar membrane extension harboring a dynamic branched actin network. Recent studies have shown that T plastin has the potential to be a Sezary cell-specific marker valuable for diagnostic and treatment of Sezary syndrome.

Function : Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Subunit : Monomer.

Subcellular Location : Cytoplasm.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR

Similarity : Contains 2 actin-binding domains.

Contains 4 CH (calponin-homology) domains.

Contains 2 EF-hand domains.

絲束(纖毛素)是肌動蛋白結(jié)合蛋白家族的成員,存在于細(xì)胞支架的重組中. 絲束3是來源于纖毛素蛋白家族的T淋巴細(xì)胞特異肌動蛋白成束蛋白。近年來T Plastin在用于sezary cell綜合征的研究方面較多。

實(shí)驗(yàn)流程：

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絲束蛋白T Plastin抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。

技術(shù)外包服務(wù):

★絲束蛋白T Plastin抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

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