中文名稱  網(wǎng)蛋白抗體品牌 

英文名稱  Anti-Plectin

別    名 EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; PLEC1; PLEC1b; Plectin 1 plectin 1 intermediate filament binding protein 500kDa; Plectin 6; PLTN.

產(chǎn)品屬性：

網(wǎng)蛋白抗體品牌 濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 細(xì)胞骨架

蛋白分子量  predicted molecular weight: 515kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Plectin

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

網(wǎng)蛋白抗體品牌 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Plectin interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the crosslinking and stabilization of cytoskeletal intermediate filaments network, but also in the regulation of their dynamics.

Function : Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.

Subunit : Homodimer or homotetramer. Interacts (via actin-binding domain) with Nesprin-3. Interacts (via CH 1 domain) with VIM (via rod region). Interacts with FER (By similarity). Interacts with COL17A1 (via N-terminus). Interacts (via N-terminus) with DST isoform 1 (via N-terminus).

Subcellular Location : Cytoplasm, cytoskeleton. Cell junction, hemidesmosome.

Post-translational modifications : Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE : Defects in PLEC are the cause of epidermolysis bullosa simplex with pyloric atresia (EBS-PA) [MIM:612138]. EBS-PA is an autosomal recessive genodermatosis characterized by severe skin blistering at birth and congenital pyloric atresia. Death usually occurs in infancy. This disorder is allelic to MD-EBS.

Similarity : Belongs to the plakin or cytolinker family.

Contains 1 actin-binding domain.

Contains 2 CH (calponin-homology) domains.

Contains 33 plectin repeats.

Contains 4 spectrin repeats.

網(wǎng)蛋白(plectin)在介導(dǎo)、維持、穩(wěn)定細(xì)胞骨架中起到關(guān)鍵作用，在微管、微絲和中間絲之間的連接中具有結(jié)構(gòu)性功能。

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

網(wǎng)蛋白抗體品牌 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

技術(shù)外包服務(wù):

★網(wǎng)蛋白抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

正在熱銷的相關(guān)產(chǎn)品：

Anti-OX40/CD134/TNFRSF4/FITC 熒光素標(biāo)記CD134抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-IL-1RA (Interleukin-1 receptor antagonist) /FITC 熒光素標(biāo)記兔抗人、大、小鼠、豬、、羊、犬白介素-1受體拮抗劑抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CD38 CD38抗體 規(guī)格 0.1ml

NIT2 (nitrilase family, member 2) NIT2蛋白抗原 0.5mg

IFNA10 英文名稱： 干擾素α10抗體 0.2ml

Rhesus antibody Rh SV2A 突觸泡蛋白2A抗體 規(guī)格 0.2ml

Anti-IL-1RA (Interleukin-1 receptor antagonist) /FITC 熒光素標(biāo)記兔抗人、大、小鼠、豬、、羊、犬白介素-1受體拮抗劑抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Human lymphocyte function associated antigen 3,LFA-3 ELISA Kit 人淋巴細(xì)胞功能相關(guān)抗原3Multi-class antibodies規(guī)格： 48T

Anti-Phospho-RSK2 (Tyr529) 磷酸化核糖體S6激酶RSK2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GRP75 G蛋白偶聯(lián)受體75抗體 規(guī)格 0.2ml

histon-H2b(Mouse histon-H2b) ELISA Kit 小鼠組蛋白H2b 96T

Noelin 英文名稱： 嗅球蛋白1抗體 0.2ml

BACH2 英文名稱： 轉(zhuǎn)錄調(diào)節(jié)蛋白BACH2抗體 0.2ml

Anti-Phospho-RSK2 (Tyr529) 磷酸化核糖體S6激酶RSK2抗體Multi-class antibodies規(guī)格： 0.1ml

AP12 ELISA Kit 大鼠apelin 12Multi-class antibodies規(guī)格： 48T

Anti-VAMP-2 囊泡相關(guān)膜蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh GATA-5 GATA結(jié)合蛋白5抗體 規(guī)格 0.1ml

P-Selectin/CD62P(Mouse P-Selectin) ELISA kit 小鼠P選擇素 96T

Phospho-NFKB1 (Thr931) 英文名稱： 磷酸化細(xì)胞核因子p50/k基因結(jié)合核因子抗體 0.1ml

AEV polyprotein 英文名稱： 禽腦脊髓炎病毒AEV抗體 0.1ml

Anti-VAMP-2 囊泡相關(guān)膜蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0071CT26.WT(小鼠細(xì)胞)5×106cells/瓶×2

CD3E Others Cynomolgus 食蟹猴 CD3e / CD3 epsilon 人細(xì)胞裂解液 (陽性對照)

小鼠平滑肌細(xì)胞完全培養(yǎng)基 100mL

Y1小鼠腎上腺皮質(zhì)瘤細(xì)胞 Y1 mouse adrenocortical tumor cells RPMI-1640(GIBCO)+10%FBS

LEP Protein Human 重組人 Leptin 蛋白

SF17(人細(xì)胞) 5×106cells/瓶×2 RT4(細(xì)胞)

CL-0068COS-1(非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞)5×106cells/瓶×2

PTPRC Others Mouse 小鼠 CD45 / PTPRC 人細(xì)胞裂解液 (陽性對照)

CCCSCK

8910PM細(xì)胞，人細(xì)胞 小鼠細(xì)胞,EMT6細(xì)胞 腎小管上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

Dami(人巨核細(xì)胞細(xì)胞) 5×106cells/瓶×2

HTEpC Pellet 人類氣管上皮細(xì)胞團(tuán)塊 > 1 mio.cells 人骨骼肌細(xì)胞cDNAHSkMC cDNA

網(wǎng)蛋白抗體品牌 TH Others Human 人 TH / Tyrosine Hydroxylase 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人臍帶間充質(zhì)干細(xì)胞cDNAHUMSC cDNA

大鼠雪旺細(xì)胞;RSC96

LTEP-a-2細(xì)胞， 人喉表皮樣癌細(xì)胞,HEp-2細(xì)胞 tTA基因修飾的小鼠*癌細(xì)胞(B類);Pan02-CAG-tTA-4C5

雜交瘤(B類);Z1510A2G6A2F8

INSR Others Human 人 Insulin Receptor / INSR / CD220 ( Short Isoform ) 人細(xì)胞裂解液 (陽性對照)