產(chǎn)品訂購信息：
英文名稱  Anti-p95 NBS1 

中文名稱   DNA修復(fù)蛋白NBS1抗體費(fèi)用 

別    名  p95 NBS1; Nijmegen breakage syndrome 1; Nijmegen breakage syndrome 1 (nibrin); AT V1; AT V2; ATV; Cell cycle regulatory protein p95; FLJ10155; MGC87362; MGC93174; NBN; NBS 1; NBS; NBS1; Nibrin; Nijmegen breakage syndrome; Nijmegen breakage syndrome protein 1; p95; NBN_HUMAN; Nibrin; Nijmegen breakage syndrome protein 1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 85kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NBS1 C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

DNA修復(fù)蛋白NBS1抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.

Function : Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection.

Subunit : Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A (By similarity). Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with HJURP, INTS3, KPNA2 and TERF2. Interacts with RBBP8; the interaction links the role of the MRN complex in DNA double-strand break sensing to resection. Interacts with SP100; recruits NBN to PML bodies.

Subcellular Location : Nucleus (By similarity). Nucleus, PML body. Chromosome, telomere (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

Post-translational modifications : Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

DISEASE : Nijmegen breakage syndrome (NBS) [MIM:251260]: A disorder characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies. Note=The disease is caused by mutations affecting the gene represented in this entry.

Breast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Aplastic anemia (AA) [MIM:609135]: A form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements. It is characterized by peripheral pancytopenia and marrow hypoplasia. Note=Disease susceptibility may be associated with variations affecting the gene represented in this entry.

Note=Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).

Similarity : Contains 1 BRCT domain.

Contains 1 FHA domain.

Database links : UniProtKB/Swiss-Prot: O60934.1

NBS1蛋白可與Mre11和Rad50蛋白形成一個Mre11-Rad50-NBS1復(fù)合物,在DNA的復(fù)制和DNA雙鏈斷裂的修復(fù)等過程中起重要作用。

anti-p- Beta catenin/FITC 熒光素標(biāo)記磷酸化β-連環(huán)蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-HCN4/FITC 熒光素標(biāo)記環(huán)化核苷酸調(diào)控陽離子通道蛋白亞型4IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CENPP 著絲粒蛋白P抗體 規(guī)格 0.2ml

Erdj5/DNAJC10 Erdj5/DNAJC10多肽 0.5mg

IFN gamma 英文名稱： 干擾素-γ/IFN-γ抗體 0.1ml

Rhesus antibody Rh TIP47/M6PRBP1 脂滴相關(guān)蛋白TIP47抗體 規(guī)格 0.2ml

Anti-HCN4/FITC 熒光素標(biāo)記環(huán)化核苷酸調(diào)控陽離子通道蛋白亞型4IgGMulti-class antibodies規(guī)格： 0.2ml

Human major histocompatibility complex II ,MHC II /HLA- II ELISA Kit 人主要組織相容性復(fù)合體Ⅱ類Multi-class antibodies規(guī)格： 48T

Anti-Phospho-RSK3 (Thr353/356) 磷酸化核糖體蛋白S6激酶家族RSK3抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GRO gamma/CXCL3 生長調(diào)節(jié)致癌基因gamma(GROγ)抗體 規(guī)格 0.1ml

F VIII Ag ELISA Kit 大鼠第八因子相關(guān)抗原 96T

OPRS1 英文名稱： 衰老相關(guān)Sigma受體蛋白抗體 0.2ml

phospho-B Raf (Thr598) 英文名稱： 磷酸化B-Raf抗體 0.1ml

Anti-Phospho-RSK3 (Thr353/356) 磷酸化核糖體蛋白S6激酶家族RSK3抗體Multi-class antibodies規(guī)格： 0.1ml

Human Acylation Stimulating Protein,ASP ELISA Kit 人酰化刺激蛋白Multi-class antibodies規(guī)格： 48T

Anti-Substance P P物質(zhì)抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Goat Anti-Mouse IgG/PE-Cy5.5 PE-Cy5.5標(biāo)記的羊抗小鼠IgG 規(guī)格 0.1ml

MC Ab ELISA Kit 大鼠黑色素細(xì)胞抗體 96T

Phospho-NFKB1(Ser932) 英文名稱： 磷酸化細(xì)胞核因子p50/k基因結(jié)合核因子抗體 0.1ml

Bax beta 英文名稱： BCL2相關(guān)X蛋白抗體 0.2ml

Anti-Substance P P物質(zhì)抗體Multi-class antibodies規(guī)格： 0.1ml

CL-0074DLD-1(人腺癌細(xì)胞)5×106cells/瓶×2

FCGR3B Others Human 人 CD16b / FCGR3B CHO細(xì)胞裂解液 (陽性對照)

EndoFectagen? 內(nèi)皮細(xì)胞轉(zhuǎn)染試劑盒

牛陀螺狀細(xì)胞;BT 雞細(xì)胞，DT40細(xì)胞 Kert-3細(xì)胞，小鼠細(xì)胞

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞(傣族);KM9403

FABP4 Others Mouse 小鼠 FABP4 / ALBP 人細(xì)胞裂解液 (陽性對照)

CL-0072Daudi(人細(xì)胞)5×106cells/瓶×2

SDF2 Others Human 人 SDF2 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

細(xì)胞凋亡蛋白酶-3分析試劑盒CAS

HT-29細(xì)胞，人細(xì)胞 人胚腎細(xì)胞,293細(xì)胞 腎系膜細(xì)胞Many types of cells包裝：5 ×105次方(1ml)

家兔骨髓間充質(zhì)干細(xì)胞;2012083MSC

SFRP4 Others Human 人 sFRP4 人細(xì)胞裂解液 (陽性對照)

DNA修復(fù)蛋白NBS1抗體費(fèi)用 MBL2 Others Human 人 MBL2 / MBL / COLEC1 CHO細(xì)胞裂解液 (陽性對照)

CL-0090Hacat(人永生化角質(zhì)形成細(xì)胞)5×106cells/瓶×2

人神經(jīng)細(xì)胞裂解物HNL

牛腎細(xì)胞;MDBK 人咽鱗癌細(xì)胞，F(xiàn)aDu細(xì)胞 Nb2-11細(xì)胞，小鼠細(xì)胞

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KM932

THSD1 Others Mouse 小鼠 THSD1 / TMTSP 人細(xì)胞裂解液 (陽性對照)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. DNA修復(fù)蛋白NBS1抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）DNA修復(fù)蛋白NBS1抗體費(fèi)用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。