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磷酸化肽基脯氨酞順反異構(gòu)酶Pinl抗體費(fèi)用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

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磷酸化肽基脯氨酞順反異構(gòu)酶Pinl抗體費(fèi)用 英文名稱  Anti-Phospho-Pin1 (Ser16)

中文名稱  磷酸化肽基脯氨酞順反異構(gòu)酶Pinl抗體費(fèi)用 

別    名 DOD; NIMA interacting 1; Peptidyl prolyl cis trans isomerase NIMA interacting 1; Peptidyl prolyl cis/trans isomerase NIMA interacting; Pin 1; PPIase Pin1; Prolyl isomerase; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1; Protein NIMA interacting 1; Rotamase Pin1; UBL 5; UBL5; PIN1_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域     免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 18kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human Pin1 around the phosphorylation site of Ser16

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化肽基脯氨酞順反異構(gòu)酶Pinl抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Pin1 is a Peptidyl-prolyl isomerases (PPIase). Peptidyl-prolyl isomerases (PPIase) facilitate the cis-trans interconversion of the peptidyl-prolyl bond thereby affecting protein folding. Pin1 is a PPIase which specifically recognizes phosphorylated S/T-P bonds. Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration.

Function : Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation.

Subunit : Interacts with STIL (By similarity). Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1.

Subcellular Location : Nucleus. Nucleus speckle. Cytoplasm.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation.

Similarity : Contains 1 PpiC domain.

Contains 1 WW domain.

Database links : UniProtKB/Swiss-Prot: Q13526.1

肽基脯氨酞順反異構(gòu)酶Pinl，是調(diào)節(jié)細(xì)胞有絲分裂相關(guān)蛋白酶，對(duì)細(xì)胞周期運(yùn)行起著非常重要的調(diào)控作用。近年來研究表明，肽基脯氨酞順反異構(gòu)酶Pinl在多種中過表達(dá)，是多個(gè)致癌信號(hào)通路的關(guān)鍵效應(yīng)分子，可加強(qiáng)和催化多種致癌信號(hào)，使其無限放大，最終引起細(xì)胞轉(zhuǎn)化和增殖失控。因次，肽基脯氨酞順反異構(gòu)酶Pinl越來越引起人們關(guān)注，被稱為發(fā)展的催化分子。近來該蛋白用于研究的也日漸增多。

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技術(shù)外包服務(wù):

★磷酸化肽基脯氨酞順反異構(gòu)酶Pinl抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。