技術(shù)外包服務(wù):

★樁蛋白Paxillin抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。

實(shí)驗(yàn)流程：

樁蛋白Paxillin抗體費(fèi)用 英文名稱  Anti-Paxillin

中文名稱  樁蛋白Paxillin抗體費(fèi)用 

別    名 Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; FLJ16691; PAXI_HUMAN; Paxillin; PXN protein.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域     免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 68kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Paxillin

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

樁蛋白Paxillin抗體費(fèi)用 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Function : Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Subunit : Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6.

Subcellular Location : Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery.

Similarity : Belongs to the paxillin family.

Contains 4 LIM zinc-binding domains.

Database links : UniProtKB/Swiss-Prot: P49023.3

Paxillin是一種分子量為68KDa的局部粘附蛋白，參與細(xì)胞的粘附與轉(zhuǎn)移。主要用于各種的研究。 Paxillin 蛋白是胞外基質(zhì)細(xì)胞粘附（粘著斑）位點(diǎn)上肌動蛋白-膜附著的細(xì)胞骨架蛋白。 Paxillin 在整合蛋白的信號傳導(dǎo)中起著非常重要的作用，整合蛋白介導(dǎo)的細(xì)胞骨架的再組織需要，paxillin 酪氨酸殘基的磷酸化。 Paxillin 被粘著斑激酶 (FAK)在其118位的酪氨酸殘基處磷酸化。已經(jīng)證明，paxillin 118位和31位兩個(gè)酪氨酸的磷酸化對于MM1癌細(xì)胞的遷移是重要的。Paxillin在胚胎發(fā)育、和多種中發(fā)揮作用。

全、新、優(yōu)、品、好四大特點(diǎn)：

樁蛋白Paxillin抗體費(fèi)用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

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