產(chǎn)品訂購信息：
英文名稱  Anti-PSD93 

中文名稱   突觸后密度蛋白93抗體費用 

別    名  Channel associated protein of synapse 110; Channel associated protein of synapses 110kD; Channel-associated protein of synapse-110; Chapsyn 110; Chapsyn-110; Chapsyn110; discs large homolog 2; Discs, large homolog 2 (Drosophila); Disks large homolog 2; DKFZp781D1854; DKFZp781E0954; Dlg 2; dlg2; DLG2_HUMAN; Dlgh 2; Dlgh2; FLJ37266; Gm1197; MGC131811; Postsynaptic density protein PSD 93; Postsynaptic density protein PSD-93; Postsynaptic density protein PSD93; PSD 93; PSD93.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞類型標(biāo)志物

蛋白分子量  predicted molecular weight: 98kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PSD93 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

突觸后密度蛋白93抗體費用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Function : Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses (By similarity).

Subunit : Interacts through its PDZ domains with NETO1. Interacts with NOS1/nNOS through second PDZ domain (By similarity). Interacts with KCNJ2/Kir2.1 (via C-terminus) through one of its PDZ domains. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Interacts with FASLG.

Subcellular Location : Membrane; Lipid-anchor (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Concentrated in soma and postsynaptic density of a subset of neurons (By similarity).

Similarity : Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

Database links : UniProtKB/Swiss-Prot: Q15700.3

Anti-CD146(MCAN)/FITC 熒光素標(biāo)記抗細(xì)胞粘付分子CD146抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-CD122/IL-2RB/FITC 熒光素標(biāo)記CD122/白介素2受體β鏈抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CD336/NKp44/NCR2 細(xì)胞毒性受體NK-p44抗體 規(guī)格 0.2ml

Nephrin Protein 去氧 0.5mg

IFI78 英文名稱： 干擾素誘導(dǎo)蛋白P78抗體 0.1ml

Rhesus antibody Rh SULT2A1 膽鹽磺基轉(zhuǎn)移酶 規(guī)格 0.1ml

Anti-CD122/IL-2RB/FITC 熒光素標(biāo)記CD122/白介素2受體β鏈抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-IRAK1 (Thr209) /FITC 熒光素標(biāo)記磷酸化白介素-1受體相關(guān)激酶1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-SATB1 (Ser47)/FITC 熒光素標(biāo)記磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ANKRD12 錨蛋白重復(fù)結(jié)構(gòu)域蛋白12抗體 規(guī)格 0.2ml

Rabbit Anti-MK IgM/PE PE標(biāo)記的兔抗猴IgM 0.1ml

GABPB2 英文名稱： 核呼吸因子2抗體 0.1ml

Rhesus antibody Rh Rabbit Anti-human IgG F(ab')2/Alexa Fluor 350 Alexa Fluor 350標(biāo)記的兔抗人IgG F(ab')2 規(guī)格 0.1ml

Anti-Phospho-SATB1 (Ser47)/FITC 熒光素標(biāo)記磷酸化核基質(zhì)結(jié)合區(qū)結(jié)合蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

AIF ELISA Kit 大鼠凋亡誘導(dǎo)因子Multi-class antibodies規(guī)格： 48T

Anti-TEM8 內(nèi)皮標(biāo)志物8抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Goat Anti-Bovine IgG/Cy5.5 Cy5.5標(biāo)記的羊抗IgG 規(guī)格 0.1ml

ALP-B(Mouse Bone-specific Alkphase B) ELISA Kit 小鼠骨特異性堿性磷酸酶B 96T

Phospho-NMDAR1(Ser896) 英文名稱： 磷酸化谷酸受體1抗體 0.1ml

Beta-lactoglobulin 英文名稱： β-乳球蛋白抗體 1ml

Anti-TEM8 內(nèi)皮標(biāo)志物8抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0098HCT-8(人盲腸腺癌細(xì)胞)5×106cells/瓶×2

RSPO1 Others Human 人 R-Spondin 1 / RSPO1 CHO細(xì)胞裂解液 (陽性對照)

人小腦星形膠質(zhì)細(xì)胞裂解物HA-c L

新生牛眼Tenon's囊成纖維細(xì)胞;NBTF 腺癌細(xì)胞，RKO細(xì)胞 TA2細(xì)胞，小鼠自發(fā)高癌細(xì)胞

人原胚腎轉(zhuǎn)化細(xì)胞;293 Ad5

TIMP1 Others Mouse 小鼠 TIMP1 / TIMP / CLGI 人細(xì)胞裂解液 (陽性對照)

CL-0096HCT 116(人細(xì)胞)5×106cells/瓶×2

SPG21 Others Human 人 SPG21 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對照)

人神經(jīng)束膜細(xì)胞裂解物HPCL

TT細(xì)胞，人骨髓樣細(xì)胞 人腎小球系膜細(xì)胞,HMC細(xì)胞 神經(jīng)小膠質(zhì)細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

兔腎細(xì)胞;RK13

SLIK1 Others Human 人 SLIK1 人細(xì)胞裂解液 (陽性對照)

突觸后密度蛋白93抗體費用 CN2 Others Mouse 小鼠 Coactin 2 / CN2 人細(xì)胞裂解液 (陽性對照)

腦動脈平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

大鼠主動脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

PC-12(高分化)大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞(高分化) PC-12 (high differeiation) rat pheochromocytoma cells (high differeiation) RPMI-1640(GIBCO)+10%FBS

IFNA8 Protein Human 重組人 Ierferon alpha-B / IFNA8 蛋白 (His 標(biāo)簽)

HHCC(人細(xì)胞) 5×106cells/瓶×2 粘質(zhì)沙雷伯氏菌

抗體的生物素化標(biāo)記實驗要點：

1. 突觸后密度蛋白93抗體費用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）突觸后密度蛋白93抗體費用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。