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親環(huán)蛋白（親環(huán)素）PPIG抗體說明書 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

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親環(huán)蛋白（親環(huán)素）PPIG抗體說明書 英文名稱  Anti-PPIG

中文名稱  親環(huán)蛋白（親環(huán)素）PPIG抗體說明書 

別    名 CARS cyclophilin; CARS Cyp; CARS-cyclophilin; CARS-Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Clk-associating RS-cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G; Peptidyl-prolyl cis-trans isomerase G; Peptidyl-prolyl isomerase G; Peptidylprolyl isomerase G (cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Ppig; PPIG_HUMAN; Rotamase G; RotamaseG; SCAF10; SR cyclophilin; SR cyp; SR related CTD associated factor 10; SR-cyclophilin; SR-cyp; SRcyp.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 89kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PPIG (361-405aa)

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

親環(huán)蛋白（親環(huán)素）PPIG抗體說明書 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PPIG belongs to the the peptidyl-prolyl cis/trans isomerase protein family (PPIases). PPIases accelerate the folding of proteins. PPIG catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is distributed in nuclear speckles, a nuclear compartment rich in splicing factors, and colocalizes with the splicing factors SC35 and pinin, suggesting a role in the regulation of pre-mRNA splicing.

Function : PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.

Subunit : Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II.

Subcellular Location : Nucleus matrix. Nucleus speckle. Note=Colocalizes with RNA splicing factors at nuclear speckles.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Contains 1 PPIase cyclophilin-type domain.

Database links : UniProtKB/Swiss-Prot: Q13427.2

肽基脯氨?；樂串悩?gòu)酶，廣泛存在于各種組織和器官。根據(jù)對劑的敏感性，分屬三個獨立的家族：親環(huán)蛋白PPIG、FK506結(jié)合蛋白和細蛋白。能夠加速自身折疊和體內(nèi)新生肽鏈折疊及變性蛋白質(zhì)的體外折疊，主要分布于內(nèi)質(zhì)網(wǎng)。

實驗流程：

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★親環(huán)蛋白（親環(huán)素）PPIG抗體說明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

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