全、新、優(yōu)、品、好四大特點(diǎn)：

磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶抗體說(shuō)明書 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。

磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶抗體說(shuō)明書 英文名稱  Anti-Phospho-PBK/TOPK(Thr9)

中文名稱  磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶抗體說(shuō)明書 

別    名 Cancer/testis antigen 84; CT84; FLJ14385; Lymphokine activated killer T cell originated protein kinase; MAPKK like protein kinase; PDZ-binding-kinase/T-LAK cell-originated protein kinase； Nori 3; Nori3; PDZ binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; SPK; T LAK cell originated protein kinase; TOPK.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域     免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 36kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human PBK/TOPK around the phosphorylation site of Thr9

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶抗體說(shuō)明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.

PBK/TOPK是一種新近發(fā)現(xiàn)的絲-蘇氨酸激酶,屬于MAPKK分子家族成員，具有參與調(diào)控細(xì)胞的增殖和周期變化, 轉(zhuǎn)化, 并且通過(guò)MAPKK信號(hào)通路參與調(diào)控細(xì)胞DNA損傷修復(fù).

實(shí)驗(yàn)流程：

正在熱銷的相關(guān)產(chǎn)品：

Anti-Calpain 2/FITC 熒光素標(biāo)記鈣蛋白酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-GZMA/FITC 熒光素標(biāo)記顆粒酶A抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Chemerin/TIG2 視黃酸受體應(yīng)答蛋白2抗體 規(guī)格 0.1ml

UCN(Urocortin)mouse ret 新型活性因子UCN抗原(小鼠、大鼠) 0.5mg

IER5L 英文名稱： 即刻早期應(yīng)答基因5樣蛋白抗體 0.2ml

Rhesus antibody Rh TMPRSS4 膜型絲氨酸蛋白酶2抗體 規(guī)格 0.2ml

Anti-GZMA/FITC 熒光素標(biāo)記顆粒酶A抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Integrin- Beta3/ITG- Beta3/CD61/FITC 熒光素標(biāo)記整合素-β3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-phospho-SHC (Tyr239/240)/FITC 熒光素標(biāo)記磷酸化SH2結(jié)構(gòu)域轉(zhuǎn)化蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh AMPK beta 1 腺苷單磷酸活化蛋白激酶β1抗體 規(guī)格 0.1ml

Rabbit Anti-mouse IgG-Fc/Gold 膠體金標(biāo)記的兔抗小鼠IgG-Fc 0.5ml

Golgin 97 英文名稱： 高爾基體蛋白97抗體 0.1ml

Rhesus antibody Rh Rabbit Anti-horse IgG/PE PE標(biāo)記的兔抗馬IgG 規(guī)格 0.1ml

Anti-phospho-SHC (Tyr239/240)/FITC 熒光素標(biāo)記磷酸化SH2結(jié)構(gòu)域轉(zhuǎn)化蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Col IV ELISA KIT 大鼠Ⅳ型膠原Multi-class antibodies規(guī)格： 48T

Anti-Phospho-TrkA (Tyr785)/TrkB (Tyr816) 磷酸化酪氨酸激酶A抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GIMAP1 GTP酶IMAP家族成員1抗體 規(guī)格 0.2ml

ENK(Human enkephalin) ELISA Kit 人腦啡肽 96T

Phospho-Nucleophosmin (Ser4) 英文名稱： 磷酸化核仁磷酸蛋白抗體 0.1ml

Apaf1 Interacting Protein 英文名稱： 凋亡蛋白活性因子1相互作用蛋白抗體 0.2ml

Anti-Phospho-TrkA (Tyr785)/TrkB (Tyr816) 磷酸化酪氨酸激酶A抗體Multi-class antibodies規(guī)格： 0.1ml

CL-0132KG-1(人)5×106cells/瓶×2

TNFRSF14 Others Cynomolgus 食蟹猴 HVEM / TNFRSF14 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人氣管上皮細(xì)胞裂解物HTEpiCL

Sw626細(xì)胞，人細(xì)胞 鮭魚胚胎細(xì)胞,RTG細(xì)胞 破骨細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

BT-20(人癌細(xì)胞) 5×106cells/瓶×2

HBSMC Pellet 人平滑肌細(xì)胞團(tuán)塊 > 1 mio.cells 人間充質(zhì)干細(xì)胞-脂肪裂解物HMSC-ad L

CL-0130K-562(人細(xì)胞)5×106cells/瓶×2

ADAM15 Others Human 人 ADAM15 CHO細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人肺泡上皮細(xì)胞裂解物HPAEpiCL

鼬肺上皮細(xì)胞;MV-1-Lu *癌細(xì)胞，PANC-1細(xì)胞 3T3 clone A31細(xì)胞，小鼠胚胎成纖維細(xì)胞

人正?；|(zhì)永生化細(xì)胞;WPMY-1

IFNAR1 Others Mouse 小鼠 IFNAR1 / IFNAR 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶抗體說(shuō)明書 CD300A Others Mouse 小鼠 CD300A 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

615小鼠株;Ca759

M14 人細(xì)胞

MDA-MB-468人癌細(xì)胞 Human breast cancer cell line MDA-MB-468 L-15+10%FBS

FAM3B Protein Human 重組人 FAM3B 蛋白 (Fc 標(biāo)簽)

人胚皮膚成纖維細(xì)胞;CCC-ESF-1 人肺微內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

技術(shù)外包服務(wù):

★磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶抗體說(shuō)明書 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。